ID   NCAP_EBLV1              Reviewed;         451 AA.
AC   A4UHP8;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   23-FEB-2022, entry version 52.
DE   RecName: Full=Nucleoprotein;
DE            Short=NP;
DE   AltName: Full=Nucleocapsid protein;
DE            Short=Protein N;
GN   Name=N;
OS   European bat lyssavirus 1 (strain Bat/Germany/RV9/1968) (EBLV1).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae;
OC   Lyssavirus.
OX   NCBI_TaxID=453115;
OH   NCBI_TaxID=40674; Mammalia.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=17374776; DOI=10.1099/vir.0.82692-0;
RA   Marston D.A., McElhinney L.M., Johnson N., Muller T., Conzelmann K.K.,
RA   Tordo N., Fooks A.R.;
RT   "Comparative analysis of the full genome sequence of European bat
RT   lyssavirus type 1 and type 2 with other lyssaviruses and evidence for a
RT   conserved transcription termination and polyadenylation motif in the G-L 3'
RT   non-translated region.";
RL   J. Gen. Virol. 88:1302-1314(2007).
CC   -!- FUNCTION: Encapsidates the genome, protecting it from nucleases. If
CC       expressed without protein P it binds non-specifically RNA and therefore
CC       can bind it's own mRNA. Interaction with protein P abolishes any non-
CC       specific RNA binding, and prevents phosphorylation. The soluble N-P
CC       complex encapsidates specifically the genomic RNA, with protein N
CC       protecting the genome like a pearl necklace. The encapsidated genomic
CC       RNA is termed the nucleocapsid (NC) and serves as template for viral
CC       transcription and replication. Protein N binds protein P in the NC
CC       through a different interaction, and can be phosphorylated. Subsequent
CC       viral replication is dependent on intracellular concentration of newly
CC       synthesized protein N. During replication, encapsidation by protein N
CC       is coupled to RNA synthesis and all replicative products are resistant
CC       to nucleases (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC       genomic RNA (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm {ECO:0000250}.
CC   -!- PTM: Phosphorylated by host. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the lyssavirus nucleocapsid protein family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EF157976; ABO65243.1; -; Genomic_RNA.
DR   RefSeq; YP_001285388.1; NC_009527.1.
DR   SMR; A4UHP8; -.
DR   GeneID; 5219908; -.
DR   KEGG; vg:5219908; -.
DR   Proteomes; UP000008926; Genome.
DR   GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3570.10; -; 1.
DR   Gene3D; 1.10.3610.10; -; 1.
DR   InterPro; IPR000448; Rhabdo_ncapsid.
DR   InterPro; IPR023331; Rhabdovirus_ncapsid_C.
DR   InterPro; IPR023330; Rhabdovirus_ncapsid_N.
DR   InterPro; IPR035961; Rhabdovirus_nucleoprotein-like.
DR   Pfam; PF00945; Rhabdo_ncap; 1.
DR   SUPFAM; SSF140809; SSF140809; 1.
PE   3: Inferred from homology;
KW   Capsid protein; Helical capsid protein; Host cytoplasm; Phosphoprotein;
KW   Reference proteome; Ribonucleoprotein; RNA-binding; Superantigen;
KW   Viral nucleoprotein; Virion.
FT   CHAIN           1..451
FT                   /note="Nucleoprotein"
FT                   /id="PRO_0000299093"
FT   MOD_RES         389
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   451 AA;  50755 MW;  BDBE4B2FE41A2226 CRC64;
     MDVNKVVFKV HNQLVSVRPE VISDQYEYKY PAIKDKKKPS ITLGKDPDLK TAYKSILSGM
     NAAKLDPDDV CSYLAGAMVL FEGICPEDWT SYGINIAKKG DKITPATLVD IHRTNTEGNW
     AQTGGQDLTR DPTTPEHASL VGLLLCLYRL SKIVGQNTGN YKTNVADRME QIFETAPFVK
     IVEHHTLMTT HKMCANWSTI PNFRFLAGAY DMFFARIEHL YSAIRVGTVV TAYEDCSGLV
     SFTGFIKQIN LTAREAILYF FHKNFEEEIK RMFEPGQETA VPHSYFIHFR SLGLSGKSPY
     SSNAVGHVFN LIHFVGCYMG QIRSLNATVI QSCAPHEMSV LGGYLGEEFF GKGTFERRFF
     RDEKELQDYE AAESTKVDVA LADDGTVNSD DEDFFSGDTR SPEAVYTRIM MNGGRLKRSH
     IKRYVSVSAN HQARPNSFAE FLNKTYSSDP R