NCAP_EBLV2
ID NCAP_EBLV2 Reviewed; 451 AA.
AC A4UHQ3; Q49IM7; Q49IM8; Q49IN0; Q49IN1; Q49IN2; Q66535;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 15-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=Nucleoprotein;
DE Short=NP;
DE AltName: Full=Nucleocapsid protein;
DE Short=Protein N;
GN Name=N;
OS European bat lyssavirus 2 (strain Human/Scotland/RV1333/2002) (EBLV2).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae;
OC Lyssavirus.
OX NCBI_TaxID=453116;
OH NCBI_TaxID=40674; Mammalia.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Holland/9018/1987;
RX PubMed=7778285; DOI=10.1006/viro.1995.1285;
RA Kissi B., Tordo N., Bourhy H.;
RT "Genetic polymorphism in the rabies virus nucleoprotein gene.";
RL Virology 209:526-537(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Holland/9018/1987, Isolate Holland/9375/1993,
RC Isolate Holland/94112/1989, Isolate Switzerland/02053/2002, and
RC Isolate Switzerland/9337/1993;
RX PubMed=16051841; DOI=10.1128/jvi.79.16.10487-10497.2005;
RA Davis P.L., Holmes E.C., Larrous F., Van der Poel W.H., Tjornehoj K.,
RA Alonso W.J., Bourhy H.;
RT "Phylogeography, population dynamics, and molecular evolution of European
RT bat lyssaviruses.";
RL J. Virol. 79:10487-10497(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=17374776; DOI=10.1099/vir.0.82692-0;
RA Marston D.A., McElhinney L.M., Johnson N., Muller T., Conzelmann K.K.,
RA Tordo N., Fooks A.R.;
RT "Comparative analysis of the full genome sequence of European bat
RT lyssavirus type 1 and type 2 with other lyssaviruses and evidence for a
RT conserved transcription termination and polyadenylation motif in the G-L 3'
RT non-translated region.";
RL J. Gen. Virol. 88:1302-1314(2007).
CC -!- FUNCTION: Encapsidates the genome, protecting it from nucleases. If
CC expressed without protein P it binds non-specifically RNA and therefore
CC can bind it's own mRNA. Interaction with protein P abolishes any non-
CC specific RNA binding, and prevents phosphorylation. The soluble N-P
CC complex encapsidates specifically the genomic RNA, with protein N
CC protecting the genome like a pearl necklace. The encapsidated genomic
CC RNA is termed the nucleocapsid (NC) and serves as template for viral
CC transcription and replication. Protein N binds protein P in the NC
CC through a different interaction, and can be phosphorylated. Subsequent
CC viral replication is dependent on intracellular concentration of newly
CC synthesized protein N. During replication, encapsidation by protein N
CC is coupled to RNA synthesis and all replicative products are resistant
CC to nucleases (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC genomic RNA (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm {ECO:0000250}.
CC -!- PTM: Phosphorylated by host. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the lyssavirus nucleocapsid protein family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U22847; AAA80289.1; -; Genomic_RNA.
DR EMBL; AY863403; AAX62917.1; -; Genomic_RNA.
DR EMBL; AY863404; AAX62918.1; -; Genomic_RNA.
DR EMBL; AY863405; AAX62919.1; -; Genomic_RNA.
DR EMBL; AY863407; AAX62921.1; -; Genomic_RNA.
DR EMBL; AY863408; AAX62922.1; -; Genomic_RNA.
DR EMBL; EF157977; ABO65248.1; -; Genomic_RNA.
DR RefSeq; YP_001285393.1; NC_009528.2.
DR SMR; A4UHQ3; -.
DR GeneID; 5219913; -.
DR KEGG; vg:5219913; -.
DR Proteomes; UP000007206; Genome.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3570.10; -; 1.
DR Gene3D; 1.10.3610.10; -; 1.
DR InterPro; IPR000448; Rhabdo_ncapsid.
DR InterPro; IPR023331; Rhabdovirus_ncapsid_C.
DR InterPro; IPR023330; Rhabdovirus_ncapsid_N.
DR InterPro; IPR035961; Rhabdovirus_nucleoprotein-like.
DR Pfam; PF00945; Rhabdo_ncap; 1.
DR SUPFAM; SSF140809; SSF140809; 1.
PE 3: Inferred from homology;
KW Capsid protein; Helical capsid protein; Host cytoplasm; Phosphoprotein;
KW Reference proteome; Ribonucleoprotein; RNA-binding; Viral nucleoprotein;
KW Virion.
FT CHAIN 1..451
FT /note="Nucleoprotein"
FT /id="PRO_0000297609"
FT MOD_RES 389
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000250"
FT VARIANT 44
FT /note="G -> E (in strain: Isolate Holland/9018/1987,
FT Isolate Holland/9375/1993 and Isolate Holland/94112/1989)"
FT VARIANT 125
FT /note="G -> A (in strain: Isolate Holland/9375/1993)"
FT VARIANT 126
FT /note="L -> Q (in strain: Isolate Holland/9018/1987,
FT Isolate Holland/94112/1989, Isolate Holland/9375/1993,
FT Isolate Switzerland/02053/2002 and Isolate Switzerland/
FT 9337/1993)"
FT VARIANT 134
FT /note="T -> P (in strain: Isolate Switzerland/02053/2002)"
FT VARIANT 174
FT /note="E -> A (in strain: Isolate Holland/9018/1987)"
FT VARIANT 205
FT /note="F -> S (in strain: Isolate Holland/9018/1987)"
FT VARIANT 247
FT /note="R -> T (in strain: Isolate Holland/9375/1993)"
FT VARIANT 281
FT /note="V -> I (in strain: Isolate Switzerland/9337/1993)"
FT VARIANT 417
FT /note="K -> Q (in strain: Isolate Holland/9018/1987,
FT Isolate Holland/9375/1993 and Isolate Holland/94112/1989)"
SQ SEQUENCE 451 AA; 50531 MW; 5EC77E42AD8455B6 CRC64;
MDADRIVFKV HNQLVSVKPE VIVDQYEYKY PAIKDRKKPS ITLGKAPDLN RAYKSILSGI
NAARLDPDDV CSYLAAAMAL FEGICPDDWE SYGILIARKG DKITPANLVN IQRTDVEGNW
ALAGGLDVIK DPTTAEHASL VGLLLCLYRL SKVSGQNTGN YKTNVADRME QIFETAPFVK
IVEHHTLMTT HKMCANWSTI PNFRFLAGTY DMFFSRIEHL YSAIRVGTVV TAYEDCSGLV
SFTGFIRQIN LTAKEAILYF FHKNFEEEIK RMFEPGQETA VPHSYFIHFR SLGLSGKSPY
SSNAVGHVFN LIHFVGSYMG QVRSLNATVI ATCAPHEMSV LGGYLGEEFF GKGTFERRFF
RDERELAEHE AIESTKTDVA LADDGTVNSD DEELYSGGTR TPEAVYTRIM VNGGKLKKSH
IKRYVSVSSN HQARPNSFAE FLNKTYSSDP R
