NCAP_I56A3
ID NCAP_I56A3 Reviewed; 498 AA.
AC P16980; Q20P39;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 2.
DT 02-JUN-2021, entry version 87.
DE RecName: Full=Nucleoprotein {ECO:0000255|HAMAP-Rule:MF_04070};
DE AltName: Full=Nucleocapsid protein {ECO:0000255|HAMAP-Rule:MF_04070};
DE Short=Protein N {ECO:0000255|HAMAP-Rule:MF_04070};
GN Name=NP {ECO:0000255|HAMAP-Rule:MF_04070};
OS Influenza A virus (strain A/Equine/Prague/1/1956 H7N7).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
OX NCBI_TaxID=380337;
OH NCBI_TaxID=8782; Aves.
OH NCBI_TaxID=9796; Equus caballus (Horse).
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=9709; Phocidae (true seals).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2718389; DOI=10.1016/0042-6822(89)90353-x;
RA Gammelin M., Mandler J., Scholtissek C.;
RT "Two subtypes of nucleoproteins (NP) of influenza A viruses.";
RL Virology 170:71-80(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2041090; DOI=10.1128/jvi.65.7.3704-3714.1991;
RA Gorman O.T., Bean W.J., Kawaoka Y., Donatelli I., Guo Y., Webster R.G.;
RT "Evolution of influenza A virus nucleoprotein genes: implications for the
RT origins of H1N1 human and classical swine viruses.";
RL J. Virol. 65:3704-3714(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=16439620; DOI=10.1126/science.1121586;
RA Obenauer J.C., Denson J., Mehta P.K., Su X., Mukatira S., Finkelstein D.B.,
RA Xu X., Wang J., Ma J., Fan Y., Rakestraw K.M., Webster R.G., Hoffmann E.,
RA Krauss S., Zheng J., Zhang Z., Naeve C.W.;
RT "Large-scale sequence analysis of avian influenza isolates.";
RL Science 311:1576-1580(2006).
CC -!- FUNCTION: Encapsidates the negative strand viral RNA, protecting it
CC from nucleases. The encapsidated genomic RNA is termed the
CC ribonucleoprotein (RNP) and serves as template for transcription and
CC replication. The RNP needs to be localized in the host nucleus to start
CC an infectious cycle, but is too large to diffuse through the nuclear
CC pore complex. NP comprises at least 2 nuclear localization signals that
CC are responsible for the active RNP import into the nucleus through
CC cellular importin alpha/beta pathway. Later in the infection, nclear
CC export of RNPs are mediated through viral proteins NEP interacting with
CC M1 which binds nucleoproteins. It is possible that nucleoprotein binds
CC directly host exportin-1/XPO1 and plays an active role in RNPs nuclear
CC export. M1 interaction with RNP seems to hide nucleoprotein's nuclear
CC localization signals. Soon after a virion infects a new cell, M1
CC dissociates from the RNP under acidification of the virion driven by M2
CC protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear
CC localization signals, targeting the RNP to the nucleus.
CC {ECO:0000255|HAMAP-Rule:MF_04070}.
CC -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. May bind host
CC exportin-1/XPO1. Binds to viral genomic RNA. Protein-RNA contacts are
CC mediated by a combination of electrostatic interactions between
CC positively charged residues and the phosphate backbone and planar
CC interactions between aromatic side chains and bases.
CC {ECO:0000255|HAMAP-Rule:MF_04070}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04070}. Host
CC nucleus {ECO:0000255|HAMAP-Rule:MF_04070}.
CC -!- PTM: Late in virus-infected cells, may be cleaved from a 56-kDa protein
CC to a 53-kDa protein by a cellular caspase. This cleavage might be a
CC marker for the onset of apoptosis in infected cells or have a specific
CC function in virus host interaction. {ECO:0000255|HAMAP-Rule:MF_04070}.
CC -!- SIMILARITY: Belongs to the influenza viruses nucleoprotein family.
CC {ECO:0000255|HAMAP-Rule:MF_04070}.
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DR EMBL; M22572; AAA43108.1; -; Genomic_RNA.
DR EMBL; M63748; AAA52233.1; -; Genomic_RNA.
DR EMBL; CY005803; ABB20502.1; -; Other_RNA.
DR PIR; D31470; VHIVN4.
DR SMR; P16980; -.
DR PRIDE; P16980; -.
DR Proteomes; UP000121173; Genome.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-UniRule.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0005198; F:structural molecule activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0075732; P:viral penetration into host nucleus; IEA:UniProtKB-UniRule.
DR HAMAP; MF_04070; INFV_NCAP; 1.
DR InterPro; IPR002141; Flu_NP.
DR Pfam; PF00506; Flu_NP; 1.
PE 3: Inferred from homology;
KW Capsid protein; Helical capsid protein; Host nucleus;
KW Host-virus interaction; Ribonucleoprotein; RNA-binding;
KW Viral nucleoprotein; Viral penetration into host nucleus; Virion;
KW Virus entry into host cell.
FT CHAIN 1..498
FT /note="Nucleoprotein"
FT /id="PRO_0000079067"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 1..18
FT /note="Unconventional nuclear localization signal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04070"
FT MOTIF 198..216
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04070"
FT CONFLICT 85
FT /note="T -> A (in Ref. 1; AAA43108)"
FT CONFLICT 99
FT /note="K -> R (in Ref. 1; AAA43108)"
FT CONFLICT 194
FT /note="I -> N (in Ref. 1; AAA43108)"
FT CONFLICT 374
FT /note="I -> M (in Ref. 1; AAA43108)"
FT CONFLICT 397
FT /note="S -> N (in Ref. 1; AAA43108)"
FT CONFLICT 433
FT /note="N -> T (in Ref. 1; AAA43108)"
FT CONFLICT 450
FT /note="N -> S (in Ref. 1; AAA43108)"
FT CONFLICT 496
FT /note="F -> Y (in Ref. 1; AAA43108)"
SQ SEQUENCE 498 AA; 56242 MW; A0E6FC6205F79107 CRC64;
MASQGTKRPY EQMETGGERQ NATEIRASVG KMVGGIGRFY IQMCTELKLN DYEGRLIQNS
ITIEKMVLSA FDERRNKYLE EHPNTGKDPK KTGGPIYRKR EGKWIRELIL YDKEEIRRIW
RQANNGEDAT AGLTHLMIWH SNLNDATYQR TRALVRTGMD PRMCSLMQGS TLPRRSGAAG
AAVKGIGTMV MELIRMIKRG INDRNFWRGE NGRKTRIAYE RMCNILKGKF QTAAQRAMMD
QVRESRNPGN AEIEDLIFLA RSALILRGSV AHKSCLPACV YGLIVASGYD FEREGYSLVG
VDPFKLLQNS QIFSLIRPNE NPAHKSQLVW MACHSAAFED LRVSSFIRGT KVIPRGQLST
RGIQIASNEN METIDSNTLE LRSRYWAIRT KSGGNTSQQK ASAGQISVQP TFSVQRNLPF
ERTTIMAAFT GNNEGRTSDM RTEIIRMMEN AKPDDVSFQG RGVFELSDEK ATNPIVPSFD
MSKEGSYFFG DNAEEFDN
