ID   NCAP_IRKV               Reviewed;         451 AA.
AC   Q5VKP6;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Nucleoprotein;
DE            Short=NP;
DE   AltName: Full=Nucleocapsid protein;
DE            Short=Protein N;
GN   Name=N;
OS   Irkut virus (IRKV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae;
OC   Lyssavirus.
OX   NCBI_TaxID=249583;
OH   NCBI_TaxID=187017; Murina leucogaster (Greater tube-nosed bat).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=15896400; DOI=10.1016/j.virusres.2005.03.008;
RA   Kuzmin I.V., Hughes G.J., Botvinkin A.D., Orciari L.A., Rupprecht C.E.;
RT   "Phylogenetic relationships of Irkut and West Caucasian bat viruses within
RT   the Lyssavirus genus and suggested quantitative criteria based on the N
RT   gene sequence for lyssavirus genotype definition.";
RL   Virus Res. 111:28-43(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=18514350; DOI=10.1016/j.virusres.2008.04.021;
RA   Kuzmin I.V., Wu X., Tordo N., Rupprecht C.E.;
RT   "Complete genomes of Aravan, Khujand, Irkut and West Caucasian bat viruses,
RT   with special attention to the polymerase gene and non-coding regions.";
RL   Virus Res. 136:81-90(2008).
CC   -!- FUNCTION: Encapsidates the genome in a ratio of one protein N per nine
CC       ribonucleotides, protecting it from nucleases. If expressed without
CC       protein P it binds non-specifically RNA and therefore can bind it's own
CC       mRNA. Interaction with protein P abolishes any non-specific RNA
CC       binding, and prevents phosphorylation. The soluble N-P complex
CC       encapsidates specifically the genomic RNA, with protein N protecting
CC       the genome like a pearl necklace. The encapsidated genomic RNA is
CC       termed the nucleocapsid (NC) and serves as template for viral
CC       transcription and replication. Protein N binds protein P in the NC
CC       through a different interaction, and can be phosphorylated. Subsequent
CC       viral replication is dependent on intracellular concentration of newly
CC       synthesized protein N. During replication, encapsidation by protein N
CC       is coupled to RNA synthesis and all replicative products are resistant
CC       to nucleases (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC       genomic RNA. In nucleocapsid, binds protein P and thereby positions the
CC       polymerase on the template. Protein P acts as a chaperone on free
CC       protein N to prevent it from aggregation before encapsidating genomic
CC       RNA (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm {ECO:0000250}.
CC   -!- PTM: Phosphorylated by host CK2. Unphosphorylated protein N seems to
CC       have a better affinity for leader viral promoter encapsidation.
CC       Phosphorylation of protein N in ribonucleocapsid may stabilize the
CC       interaction with protein P, thereby playing an important role in viral
CC       transcription/replication (By similarity). {ECO:0000250}.
CC   -!- MISCELLANEOUS: Displays a superantigen activity in human and mouse,
CC       activating mostly V-beta-8 subtypes of T-cell receptor. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the lyssavirus nucleocapsid protein family.
CC       {ECO:0000305}.
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DR   EMBL; EF614260; AAR03477.1; -; mRNA.
DR   RefSeq; YP_007641397.1; NC_020809.1.
DR   SMR; Q5VKP6; -.
DR   GeneID; 14857936; -.
DR   KEGG; vg:14857936; -.
DR   Proteomes; UP000008381; Genome.
DR   GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3570.10; -; 1.
DR   Gene3D; 1.10.3610.10; -; 1.
DR   InterPro; IPR000448; Rhabdo_ncapsid.
DR   InterPro; IPR023331; Rhabdovirus_ncapsid_C.
DR   InterPro; IPR023330; Rhabdovirus_ncapsid_N.
DR   InterPro; IPR035961; Rhabdovirus_nucleoprotein-like.
DR   Pfam; PF00945; Rhabdo_ncap; 1.
DR   SUPFAM; SSF140809; SSF140809; 1.
PE   2: Evidence at transcript level;
KW   Capsid protein; Helical capsid protein; Host cytoplasm; Phosphoprotein;
KW   Reference proteome; Ribonucleoprotein; RNA-binding; Superantigen;
KW   Viral nucleoprotein; Virion.
FT   CHAIN           1..451
FT                   /note="Nucleoprotein"
FT                   /id="PRO_0000295203"
FT   MOD_RES         389
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   451 AA;  50873 MW;  C789EB65E1227888 CRC64;
     MDSDRIVFKV HNQLVSLKPE VISDQYEYKY PAIDDKKKPS ITLGKAPDLK TAYKSILSGM
     NAAKLDPDDV CSYLAAAMVF FEGICPEDWT SYGINIAKKG DKITPAVLVD IQRTNTEGNW
     AQAGGQDLTR DPTTPEHASL VGLLLCLYRL SKIVGQNTGN YKTNVAERME QIFETAPFVK
     IVEHHTLMTT HKMCANWSTI PNFRFLAGVY DMFFSRIEHL YSAIRVGTVV TAYEDCSGLV
     SFTTFIRQIN LTARDAVLYF FHKNFEEEIK RMFEPGQETA VPHSYFIHFR SLGLSGKSPY
     SSNAVGHTFN LIHFVGCYMG QVRSLNATVI QSCAPHEMSV LGGYLGEEFF GRGTFERRFF
     RDEKELQDYE AAEATKIDLA LEDDGTVNSD DEDFFSGETR SPEAVYSRIM MSGGRLKKSH
     IKRYISVSSN HQARPNSFAE FLNKTYASDT R