NCAP_ISAV8
ID NCAP_ISAV8 Reviewed; 616 AA.
AC Q8V3T7;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 02-JUN-2021, entry version 64.
DE RecName: Full=Nucleoprotein {ECO:0000303|PubMed:14990725};
DE Short=NP;
DE AltName: Full=Nucleocapsid protein;
DE Short=Protein N;
DE AltName: Full=vp66;
GN Name=Segment-3 {ECO:0000303|PubMed:20979983};
OS Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99)
OS (ISAV).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Isavirus.
OX NCBI_TaxID=652965;
OH NCBI_TaxID=8049; Gadus morhua (Atlantic cod).
OH NCBI_TaxID=8019; Oncorhynchus kisutch (Coho salmon) (Salmo kisutch).
OH NCBI_TaxID=8022; Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
OH NCBI_TaxID=8060; Pollachius virens (Saithe) (Gadus virens).
OH NCBI_TaxID=8030; Salmo salar (Atlantic salmon).
OH NCBI_TaxID=8032; Salmo trutta (Brown trout).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=11807235; DOI=10.1099/0022-1317-83-2-421;
RA Clouthier S.C., Rector T., Brown N.E., Anderson E.D.;
RT "Genomic organization of infectious salmon anaemia virus.";
RL J. Gen. Virol. 83:421-428(2002).
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=14990725; DOI=10.1128/jvi.78.6.3063-3071.2004;
RA Falk K., Aspehaug V., Vlasak R., Endresen C.;
RT "Identification and characterization of viral structural proteins of
RT infectious salmon anemia virus.";
RL J. Virol. 78:3063-3071(2004).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15522447; DOI=10.1016/j.virusres.2004.06.001;
RA Aspehaug V., Falk K., Krossoy B., Thevarajan J., Sanders L., Moore L.,
RA Endresen C., Biering E.;
RT "Infectious salmon anemia virus (ISAV) genomic segment 3 encodes the viral
RT nucleoprotein (NP), an RNA-binding protein with two monopartite nuclear
RT localization signals (NLS).";
RL Virus Res. 106:51-60(2004).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=18632128; DOI=10.1016/j.virol.2008.05.036;
RA Goic B., Bustamante J., Miquel A., Alvarez M., Vera M.I., Valenzuela P.D.,
RA Burzio L.O.;
RT "The nucleoprotein and the viral RNA of infectious salmon anemia virus
RT (ISAV) are localized in the nucleolus of infected cells.";
RL Virology 379:55-63(2008).
RN [5]
RP REVIEW.
RX PubMed=20979983; DOI=10.1016/j.virusres.2010.10.021;
RA Cottet L., Rivas-Aravena A., Cortez-San Martin M., Sandino A.M.,
RA Spencer E.;
RT "Infectious salmon anemia virus--genetics and pathogenesis.";
RL Virus Res. 155:10-19(2011).
CC -!- FUNCTION: Encapsidates the negative strand viral RNA, protecting it
CC from nucleases (Probable). The encapsidated genomic RNA is termed the
CC ribonucleoprotein (RNP) and serves as template for transcription and
CC replication (PubMed:15522447). {ECO:0000269|PubMed:15522447,
CC ECO:0000305}.
CC -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC genomic RNA. Protein-RNA contacts are mediated by a combination of
CC electrostatic interactions between positively charged residues and the
CC phosphate backbone and planar interactions between aromatic side chains
CC and bases (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:14990725}. Host
CC nucleus {ECO:0000269|PubMed:15522447}. Host nucleus, host nucleolus
CC {ECO:0000269|PubMed:18632128}.
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DR EMBL; AF404345; AAL67961.1; -; Viral_cRNA.
DR RefSeq; YP_145803.1; NC_006502.1.
DR PDB; 4EWC; X-ray; 2.70 A; A=2-600.
DR PDBsum; 4EWC; -.
DR SMR; Q8V3T7; -.
DR GeneID; 3170816; -.
DR KEGG; vg:3170816; -.
DR Proteomes; UP000008772; Genome.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0075732; P:viral penetration into host nucleus; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW 3D-structure; Capsid protein; Helical capsid protein; Host nucleus;
KW Reference proteome; Viral nucleoprotein;
KW Viral penetration into host nucleus; Virion; Virus entry into host cell.
FT CHAIN 1..616
FT /note="Nucleoprotein"
FT /id="PRO_0000403915"
FT MOTIF 230..233
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:15522447"
FT MOTIF 473..476
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:15522447"
FT STRAND 7..11
FT /evidence="ECO:0007829|PDB:4EWC"
FT STRAND 13..16
FT /evidence="ECO:0007829|PDB:4EWC"
FT STRAND 18..24
FT /evidence="ECO:0007829|PDB:4EWC"
FT STRAND 29..34
FT /evidence="ECO:0007829|PDB:4EWC"
FT TURN 42..44
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 65..73
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 89..107
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 109..111
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 135..155
FT /evidence="ECO:0007829|PDB:4EWC"
FT TURN 156..158
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 161..164
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 167..181
FT /evidence="ECO:0007829|PDB:4EWC"
FT STRAND 202..207
FT /evidence="ECO:0007829|PDB:4EWC"
FT STRAND 209..217
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 219..229
FT /evidence="ECO:0007829|PDB:4EWC"
FT TURN 233..235
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 246..262
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 265..268
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 272..277
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 282..288
FT /evidence="ECO:0007829|PDB:4EWC"
FT TURN 289..292
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 295..298
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 302..304
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 308..319
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 327..339
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 343..351
FT /evidence="ECO:0007829|PDB:4EWC"
FT TURN 359..361
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 362..374
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 387..395
FT /evidence="ECO:0007829|PDB:4EWC"
FT STRAND 405..407
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 410..418
FT /evidence="ECO:0007829|PDB:4EWC"
FT STRAND 422..425
FT /evidence="ECO:0007829|PDB:4EWC"
FT STRAND 427..429
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 431..442
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 450..456
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 464..466
FT /evidence="ECO:0007829|PDB:4EWC"
FT STRAND 481..484
FT /evidence="ECO:0007829|PDB:4EWC"
FT STRAND 510..512
FT /evidence="ECO:0007829|PDB:4EWC"
FT STRAND 519..521
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 523..529
FT /evidence="ECO:0007829|PDB:4EWC"
FT HELIX 543..557
FT /evidence="ECO:0007829|PDB:4EWC"
FT STRAND 561..567
FT /evidence="ECO:0007829|PDB:4EWC"
FT STRAND 570..576
FT /evidence="ECO:0007829|PDB:4EWC"
SQ SEQUENCE 616 AA; 68039 MW; 0A2908A1EA25063C CRC64;
MADKGMTYSF DVRDNTLVVR RSTATKSGIK ISYREDRGTS LLQKAFAGTE DEFWVELDQD
VYVDKKIREF LVEEKMKDMS TRVSGAVAAA IERSVEFDNF SKEAAANIEM AGVDDEEAGG
SGLVDNRRKN KGVSNMAYNL SLFIGMVFPA LTTFFSAILS EGEMSIWQNG QAIMRILALA
DEDGKRQTRT GGQRVDMADV TKLNVVTANG KVKQVEVNLN DLKAAFRQSR PKRSDYRKGQ
GSKATESSIS NQCMALIMKS VLSADQLFAP GVKMMRTNGF NASYTTLAEG ANIPSKYLRH
MRNCGGVALD LMGMKRIKNS PEGAKSKIFS IIQKKVRGRC RTEEQRLLTS ALKISDGENK
FQRIMDTLCT SFLIDPPRTT KCFIPPISSL MMYIQEGNSV LAMDFMKNGE DACKICREAK
LKVGVNSTFT MSVARTCVAV SMVATAFCSA DIIENAVPGS ERYRSNIKAN TTKPKKDSTY
TIQGLRLSNV RYEARPETSQ SNTDRSWQVN VTDSFGGLAV FNQGAIREML GDGTSETTSV
NVRALVKRIL KSASERSARA VKTFMVGEQG KSAIVISGVG LFSIDFEGVE EAERITDMTP
EIEFDEDDEE EEDIDI
