ID   NCAP_NDVAF              Reviewed;         489 AA.
AC   Q99FY3;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   02-JUN-2021, entry version 55.
DE   RecName: Full=Nucleoprotein;
DE   AltName: Full=Nucleocapsid protein;
DE            Short=NP;
DE            Short=Protein N;
GN   Name=N; Synonyms=NP;
OS   Newcastle disease virus (isolate AF2240) (NDV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Paramyxoviridae; Avulavirinae;
OC   Orthoavulavirus; Avian orthoavulavirus 1.
OX   NCBI_TaxID=351071;
OH   NCBI_TaxID=9031; Gallus gallus (Chicken).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA   Kho C.L., Tan W.S., Yusoff K.;
RT   "Sequence analysis of the nucleocapsid of a Newcastle disease virus heat
RT   resistant strain: comparison with other members of the paramyxoviridae.";
RL   J. Biochem. Mol. Biol. Biophys. 5:463-471(2001).
RN   [2]
RP   HOMOMULTIMERIZATION.
RX   PubMed=12867648; DOI=10.1099/vir.0.19107-0;
RA   Kho C.L., Tan W.S., Tey B.T., Yusoff K.;
RT   "Newcastle disease virus nucleocapsid protein: self-assembly and length-
RT   determination domains.";
RL   J. Gen. Virol. 84:2163-2168(2003).
RN   [3]
RP   INTERACTION WITH P PROTEIN.
RX   PubMed=15098113; DOI=10.1007/s00705-003-0273-8;
RA   Kho C.L., Tan W.S., Tey B.T., Yusoff K.;
RT   "Regions on nucleocapsid protein of Newcastle disease virus that interact
RT   with its phosphoprotein.";
RL   Arch. Virol. 149:997-1005(2004).
CC   -!- FUNCTION: Encapsidates the genome, protecting it from nucleases. The
CC       nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA
CC       is termed the NC and serves as template for transcription and
CC       replication. During replication, encapsidation by N is coupled to RNA
CC       synthesis and all replicative products are resistant to nucleases (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC       genomic RNA. In nucleocapsid, binds the P protein and thereby positions
CC       the polymerase on the template (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host cytoplasm.
CC   -!- DOMAIN: Ncore is globular and carries regions required for N self-
CC       assembly and RNA-binding. Ntail is an intrinsically disordered
CC       monomeric domain (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the paramyxoviruses nucleocapsid family.
CC       {ECO:0000305}.
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DR   EMBL; AF284646; AAG53089.1; -; Genomic_RNA.
DR   SMR; Q99FY3; -.
DR   GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   InterPro; IPR002021; Paramyx_ncap.
DR   Pfam; PF00973; Paramyxo_ncap; 1.
PE   1: Evidence at protein level;
KW   Capsid protein; Helical capsid protein; Host cytoplasm; Ribonucleoprotein;
KW   RNA-binding; Viral nucleoprotein; Virion.
FT   CHAIN           1..489
FT                   /note="Nucleoprotein"
FT                   /id="PRO_0000142663"
FT   REGION          1..400
FT                   /note="Ncore"
FT                   /evidence="ECO:0000250"
FT   REGION          1..375
FT                   /note="P protein-binding"
FT   REGION          400..489
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          401..489
FT                   /note="Ntail"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        403..417
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        424..441
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   489 AA;  53225 MW;  B6ABA228ADFDC5ED CRC64;
     MSSVFDEYEQ LLAAQTRPNG AHGGGERGST LRVEVPVFTL NSDDPEDRWN FAVFCLRIAV
     SEDANKPLRQ GALISLLCSH SQVMRNHVAL AGKQNEATLT VLEIDGFTSS VPQFNNRSGV
     SEERAQRFMV IAGSLPRACS NGTPFVTAGV EDDAPEDITD TLERILSIQA QVWVTVAKAM
     TAYETADESE TRRINKYMQQ GRVQKKYILH PVCRSAIQLT IRHSLAVRIF LVSELKRGRN
     TAGGSSTYYN LVGDVDSYIR NTGLTAFFLT LKYGINTKTS ALALSSLTGD IQKMKQLMRL
     YRMKGENAPY MTLLGDSDQM SFAPAEYAQL YSFAMGMASV LDKGTGKYQF ARDFMSTSFW
     RLGVEYAQAQ GSSINEDMAA ELKLTPAARR GLAAAAQRVS EETGSVDIPT QQAGVLTGLS
     DGGPRASQGG SNKSQGQPDA GDGETQFLDL MRAVANSMRE APNSAQSTTH PEPPPTPGPS
     QDNDTDWGY