NCAP_PEDV7
ID NCAP_PEDV7 Reviewed; 441 AA.
AC Q07499;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 02-JUN-2021, entry version 104.
DE RecName: Full=Nucleoprotein {ECO:0000255|HAMAP-Rule:MF_04095};
DE AltName: Full=Nucleocapsid protein {ECO:0000255|HAMAP-Rule:MF_04095};
DE Short=NC {ECO:0000255|HAMAP-Rule:MF_04095};
DE Short=Protein N {ECO:0000255|HAMAP-Rule:MF_04095};
GN Name=N {ECO:0000255|HAMAP-Rule:MF_04095}; ORFNames=6;
OS Porcine epidemic diarrhea virus (strain CV777) (PEDV).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC Alphacoronavirus; Pedacovirus.
OX NCBI_TaxID=229032;
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=8397280; DOI=10.1099/0022-1317-74-9-1795;
RA Bridgen A., Duarte M., Tobler K., Laude H., Ackermann M.;
RT "Sequence determination of the nucleocapsid protein gene of the porcine
RT epidemic diarrhea virus confirms that this virus is a coronavirus related
RT to human coronavirus 229E and porcine transmissible gastroenteritis
RT virus.";
RL J. Gen. Virol. 74:1795-1804(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=8291230; DOI=10.1006/viro.1994.1058;
RA Duarte M., Tobler K., Bridgen A., Rasschaert D., Ackermann M., Laude H.;
RT "Sequence analysis of the porcine epidemic diarrhea virus genome between
RT the nucleocapsid and spike protein genes reveals a polymorphic ORF.";
RL Virology 198:466-476(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=9782358; DOI=10.1007/978-1-4615-5331-1_101;
RA Bridgen A., Kocherhans R., Tobler K., Carvajal A., Ackermann M.;
RT "Further analysis of the genome of porcine epidemic diarrhea virus.";
RL Adv. Exp. Med. Biol. 440:781-786(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=11724265; DOI=10.1023/a:1011831902219;
RA Kocherhans R., Bridgen A., Ackermann M., Tobler K.;
RT "Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome
RT sequence.";
RL Virus Genes 23:137-144(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA Chen J.F., Feng L., Shi H.Y., Sun D.B., Tong Y.E.;
RT "Molecular characteristics of nucleocapsid protein gene of porcine epidemic
RT diarrhea virus strain CV777.";
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INTERACTION WITH HOST RSAD2, AND SUBCELLULAR LOCATION.
RX PubMed=32719955; DOI=10.1007/s00705-020-04747-8;
RA Wu J., Chi H., Fu Y., Cao A., Shi J., Zhu M., Zhang L., Hua D., Huang J.;
RT "The antiviral protein viperin interacts with the viral N protein to
RT inhibit proliferation of porcine epidemic diarrhea virus.";
RL Arch. Virol. 165:2279-2289(2020).
CC -!- FUNCTION: Packages the positive strand viral genome RNA into a helical
CC ribonucleocapsid (RNP) and plays a fundamental role during virion
CC assembly through its interactions with the viral genome and membrane
CC protein M. Plays an important role in enhancing the efficiency of
CC subgenomic viral RNA transcription as well as viral replication.
CC {ECO:0000255|HAMAP-Rule:MF_04095}.
CC -!- SUBUNIT: Homooligomer. Both monomeric and oligomeric forms interact
CC with RNA. Interacts with protein M. Interacts with NSP3; this
CC interaction serves to tether the genome to the newly translated
CC replicase-transcriptase complex at a very early stage of infection (By
CC similarity). Interacts with host RSAD2; this interaction inhibits viral
CC replication (PubMed:32719955). {ECO:0000255|HAMAP-Rule:MF_04095,
CC ECO:0000269|PubMed:32719955}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04095}. Host
CC endoplasmic reticulum-Golgi intermediate compartment
CC {ECO:0000255|HAMAP-Rule:MF_04095, ECO:0000269|PubMed:32719955}. Host
CC Golgi apparatus {ECO:0000255|HAMAP-Rule:MF_04095}. Note=Located inside
CC the virion, complexed with the viral RNA. Probably associates with ER-
CC derived membranes where it participates in viral RNA synthesis and
CC virus budding. {ECO:0000255|HAMAP-Rule:MF_04095}.
CC -!- PTM: ADP-ribosylated. The ADP-ribosylation is retained in the virion
CC during infection. {ECO:0000255|HAMAP-Rule:MF_04095}.
CC -!- PTM: Phosphorylated on serine and threonine residues.
CC {ECO:0000255|HAMAP-Rule:MF_04095}.
CC -!- SIMILARITY: Belongs to the alphacoronavirus nucleocapsid protein
CC family. {ECO:0000255|HAMAP-Rule:MF_04095}.
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DR EMBL; Z14976; CAA78697.1; -; Genomic_RNA.
DR EMBL; AF353511; AAK38660.1; -; Genomic_RNA.
DR EMBL; DQ355221; ABC84372.1; -; Genomic_RNA.
DR PIR; JQ2191; JQ2191.
DR RefSeq; NP_598314.1; NC_003436.1.
DR SMR; Q07499; -.
DR GeneID; 935179; -.
DR KEGG; vg:935179; -.
DR Proteomes; UP000008159; Genome.
DR GO; GO:0044172; C:host cell endoplasmic reticulum-Golgi intermediate compartment; IEA:UniProtKB-SubCell.
DR GO; GO:0044177; C:host cell Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR CDD; cd21595; CoV_N-CTD; 1.
DR CDD; cd21554; CoV_N-NTD; 1.
DR HAMAP; MF_04095; ALPHA_CORONA_NCAP; 1.
DR InterPro; IPR044344; N_prot_C_CoV.
DR InterPro; IPR044345; N_prot_N_CoV.
DR InterPro; IPR042548; NCAP_aCoV.
DR InterPro; IPR001218; Nucleocap_CoV.
DR InterPro; IPR037179; Nucleocapsid_C.
DR InterPro; IPR037195; Nucleocapsid_N.
DR Pfam; PF00937; CoV_nucleocap; 2.
DR PIRSF; PIRSF003888; Corona_nucleocap; 1.
DR SUPFAM; SSF103068; SSF103068; 1.
DR SUPFAM; SSF110304; SSF110304; 1.
DR PROSITE; PS51929; COV_N_CTD; 1.
DR PROSITE; PS51928; COV_N_NTD; 1.
PE 1: Evidence at protein level;
KW ADP-ribosylation; Host Golgi apparatus; Phosphoprotein; Ribonucleoprotein;
KW RNA-binding; Transcription; Transcription regulation; Viral nucleoprotein;
KW Virion.
FT CHAIN 1..441
FT /note="Nucleoprotein"
FT /id="PRO_0000283929"
FT DOMAIN 14..136
FT /note="CoV N NTD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01276"
FT DOMAIN 266..382
FT /note="CoV N CTD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01277"
FT REGION 16..146
FT /note="RNA-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04095"
FT REGION 131..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 231..278
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 277..379
FT /note="Dimerization"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04095"
FT COMPBIAS 140..216
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 234..278
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 5
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04095"
FT MOD_RES 143
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04095"
SQ SEQUENCE 441 AA; 48967 MW; 5D0692DDE78D2469 CRC64;
MASVSFQDRG RKRVPLSLYA PLRVTNDKPL SKVLANNAVP TNKGNKDQQI GYWNEQIRWR
MRRGERIEQP SNWHFYYLGT GPHGDLRYRT RTEGVFWVAK EGAKTEPTNL GVRKASEKPI
IPKFSQQLPS VVEIVEPNTP PASRANSRSR SRGNGNNRSR SPSNNRGNNQ SRGNSQNRGN
NQGRGASQNR GGNNNNNNKS RNQSNNRNQS NDRGGVTSRD DLVAAVKDAL KSLGIGENPD
RHKQQQKPKQ EKSDNSGKNT PKKNKSRATS KERDLKDIPE WRRIPKGENS VAACFGPRGG
FKNFGDAEFV EKGVDASGYA QIASLAPNVA ALLFGGNVAV RELADSYEIT YNYKMTVPKS
DPNVELLVSQ VDAFKTGNAK LQRKKEKKNK RETTLQQHEE AIYDDVGAPS DVTHANLEWD
TAVDGGDTAV EIINEIFDTG N
