ID   NCAP_PIRYV              Reviewed;         427 AA.
AC   P26037;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   23-FEB-2022, entry version 76.
DE   RecName: Full=Nucleoprotein;
DE            Short=NP;
DE   AltName: Full=Nucleocapsid protein;
DE            Short=Protein N;
GN   Name=N;
OS   Piry virus (PIRYV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae;
OC   Vesiculovirus.
OX   NCBI_TaxID=11274;
OH   NCBI_TaxID=126289; Gracilinanus microtarsus (Brazilian gracile mouse opossum).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 7-427.
RX   PubMed=1698928; DOI=10.1099/0022-1317-71-9-2191;
RA   Crysler J.G., Lee P., Reinders M., Prevec L.;
RT   "The sequence of the nucleocapsid protein (N) gene of Piry virus: possible
RT   domains in the N protein of vesiculoviruses.";
RL   J. Gen. Virol. 71:2191-2194(1990).
RN   [2]
RP   PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-60.
RX   PubMed=3023698; DOI=10.1128/jvi.61.1.200-205.1987;
RA   Nichol S.T., Holland J.J.;
RT   "Genome RNA terminus conservation and diversity among vesiculoviruses.";
RL   J. Virol. 61:200-205(1987).
CC   -!- FUNCTION: Encapsidates the genome in a ratio of one N per nine
CC       ribonucleotides, protecting it from nucleases. The encapsidated genomic
CC       RNA is termed the NC and serves as template for transcription and
CC       replication. Replication is dependent on intracellular concentration of
CC       newly synthesized N, termed N(0), which corresponds to the protein not
CC       associated with RNA. In contrast, when associated with RNA, it is
CC       termed N. During replication, encapsidation by N(0) is coupled to RNA
CC       synthesis and all replicative products are resistant to nucleases (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC       genomic RNA. N in nucleocapsid binds the P protein and thereby
CC       positions the polymerase on the template. Interaction of N(0) with the
CC       P protein prevents the uncontrolled aggregation of N(0) (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm. Note=The nucleocapsid is
CC       synthesized in the cytoplasm, and is subsequently transported via
CC       microtubules to the cell periphery. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the vesiculovirus nucleocapsid protein family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M14714; AAA48454.1; ALT_SEQ; Genomic_RNA.
DR   PIR; A36643; VHVNPV.
DR   SMR; P26037; -.
DR   GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3570.10; -; 1.
DR   Gene3D; 1.10.3610.10; -; 1.
DR   InterPro; IPR000448; Rhabdo_ncapsid.
DR   InterPro; IPR023331; Rhabdovirus_ncapsid_C.
DR   InterPro; IPR023330; Rhabdovirus_ncapsid_N.
DR   InterPro; IPR035961; Rhabdovirus_nucleoprotein-like.
DR   Pfam; PF00945; Rhabdo_ncap; 1.
DR   SUPFAM; SSF140809; SSF140809; 1.
PE   3: Inferred from homology;
KW   Capsid protein; Helical capsid protein; Host cytoplasm; Ribonucleoprotein;
KW   RNA-binding; Viral nucleoprotein; Virion.
FT   CHAIN           1..427
FT                   /note="Nucleoprotein"
FT                   /id="PRO_0000222812"
SQ   SEQUENCE   427 AA;  48435 MW;  CD6BC3AE0B6E32D1 CRC64;
     MSVSVKRIST GTQVVAALPA SEDPVEFPAE YFAKNPGKIP VFISTRLDLD KLRQYVYMGL
     TTGEVNVCHI NSYLYRVLQN IEDEARDNWV SFRVTIAAKD TTVRLFDMLE VKEYAGLVPD
     GKENANLTQT VDEWLPMYIL GLQRVGRATD ERYRGDLYDH LCAQCKLKSP SFESLGNPAD
     NNYASWINDS NFLKLVAAID MFFHHFKKHE EAVIRFGTIT SRFKDCAALS TMAHLVKVTG
     MPVEEVMTWV LNKPVEDEVC RMMTPGQEID KADSYMPYLI DFGLSTKSPY SSVKNPCFHF
     WGQLTAVLVN SARARNARVP DDIPYQELTQ AALLFAYATG RSSDLAQRFV LHNKEYIKAA
     PDASDNSERE ESKEAPTTRD VVEWLAWWDD IGQVRTRDME VFARRAVHGI LDPREKSIGA
     YARGYFA