NCAP_PRRSL
ID NCAP_PRRSL Reviewed; 128 AA.
AC Q04558;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 23-FEB-2022, entry version 66.
DE RecName: Full=Nucleoprotein;
DE AltName: Full=Nucleocapsid protein;
DE Short=Protein N;
GN Name=N; Synonyms=VP1; ORFNames=7;
OS Porcine reproductive and respiratory syndrome virus (strain Lelystad)
OS (PRRSV).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Nidovirales; Arnidovirineae; Arteriviridae; Variarterivirinae;
OC Betaarterivirus; Eurpobartevirus; Betaarterivirus suid 1.
OX NCBI_TaxID=11049;
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=8517032; DOI=10.1006/viro.1993.1008;
RA Meulenberg J.J.M., Hulst M.M., de Meijer E.J., Moonen P.L.J.M.,
RA den Besten A., de Kluyver E.P., Wensvoort G., Moormann R.J.M.;
RT "Lelystad virus, the causative agent of porcine epidemic abortion and
RT respiratory syndrome (PEARS), is related to LDV and EAV.";
RL Virology 192:62-72(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Boxmeer 10;
RX PubMed=8438574; DOI=10.1006/viro.1993.1129;
RA Conzelmann K.K., Visser N., van Woensel P., Thiel H.J.;
RT "Molecular characterization of porcine reproductive and respiratory
RT syndrome virus, a member of the arterivirus group.";
RL Virology 193:329-339(1993).
RN [3]
RP SUBUNIT, AND FUNCTION.
RC STRAIN=PA8;
RX PubMed=12663761; DOI=10.1128/jvi.77.8.4546-4557.2003;
RA Wootton S.K., Yoo D.;
RT "Homo-oligomerization of the porcine reproductive and respiratory syndrome
RT virus nucleocapsid protein and the role of disulfide linkages.";
RL J. Virol. 77:4546-4557(2003).
RN [4]
RP FUNCTION, AND INTERACTION WITH HOST HIC.
RX PubMed=19090724; DOI=10.1515/bc.2009.028;
RA Song C., Lu R., Bienzle D., Liu H.C., Yoo D.;
RT "Interaction of the porcine reproductive and respiratory syndrome virus
RT nucleocapsid protein with the inhibitor of MyoD family-a domain-containing
RT protein.";
RL Biol. Chem. 390:215-223(2009).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=20728481; DOI=10.1016/j.jbiotec.2010.08.009;
RA Zheng H., Liu C., Zhuang J., Yuan S.;
RT "Baculovirus expression of cloned porcine arterivirus generates infectious
RT particles in both insect and mammalian cells.";
RL J. Biotechnol. 150:251-258(2010).
RN [6]
RP FUNCTION.
RX PubMed=21947566; DOI=10.1007/s00705-011-1116-7;
RA Sagong M., Lee C.;
RT "Porcine reproductive and respiratory syndrome virus nucleocapsid protein
RT modulates interferon-beta production by inhibiting IRF3 activation in
RT immortalized porcine alveolar macrophages.";
RL Arch. Virol. 156:2187-2195(2011).
RN [7]
RP FUNCTION, INTERACTION WITH HOST PABPC1, AND SUBCELLULAR LOCATION.
RC STRAIN=SY0608;
RX PubMed=22985629; DOI=10.1016/j.antiviral.2012.09.004;
RA Wang X., Bai J., Zhang L., Wang X., Li Y., Jiang P.;
RT "Poly(A)-binding protein interacts with the nucleocapsid protein of porcine
RT reproductive and respiratory syndrome virus and participates in viral
RT replication.";
RL Antiviral Res. 96:315-323(2012).
CC -!- FUNCTION: Multifunctional protein which associates with itself through
CC both covalent and non-covalent interactions to provide the basis for
CC viral assembly. May play a role in the regulation of cellular gene
CC expression during infection by interacting with host HIC. Plays a role
CC in the inhibition of the dsRNA-mediated IFN induction pathway by
CC inhibiting host IRF3 phosphorylation and nuclear translocation.
CC {ECO:0000269|PubMed:12663761, ECO:0000269|PubMed:19090724,
CC ECO:0000269|PubMed:21947566, ECO:0000269|PubMed:22985629}.
CC -!- SUBUNIT: Homooligomer. Interacts with host PABPC1 (via C-terminus).
CC Interacts with host HIC; this interaction may regulate the expression
CC of different cellular genes. {ECO:0000269|PubMed:12663761,
CC ECO:0000269|PubMed:19090724, ECO:0000269|PubMed:22985629}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:20728481}. Host
CC cytoplasm {ECO:0000269|PubMed:22985629}. Host nucleus
CC {ECO:0000269|PubMed:22985629}.
CC -!- SIMILARITY: Belongs to the arteriviridae nucleocapsid family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M96262; AAA46280.1; -; Genomic_RNA.
DR EMBL; L04493; AAA47107.1; -; Genomic_RNA.
DR PIR; B44281; B44281.
DR PIR; G45392; G45392.
DR SMR; Q04558; -.
DR Proteomes; UP000006687; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0039548; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity; IEA:UniProtKB-KW.
DR InterPro; IPR002484; Arte_nucleocap.
DR InterPro; IPR037179; Nucleocapsid_C.
DR Pfam; PF01481; Arteri_nucleo; 1.
DR SUPFAM; SSF103068; SSF103068; 1.
PE 1: Evidence at protein level;
KW Host cytoplasm; Host nucleus; Host-virus interaction;
KW Inhibition of host innate immune response by virus;
KW Inhibition of host IRF3 by virus; Inhibition of host RLR pathway by virus;
KW Reference proteome; Ribonucleoprotein; RNA-binding; Viral immunoevasion;
KW Viral nucleoprotein; Virion.
FT CHAIN 1..128
FT /note="Nucleoprotein"
FT /id="PRO_0000080872"
FT REGION 1..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..41
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 45..60
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 128 AA; 13797 MW; 6B08CB696C9F53A4 CRC64;
MAGKNQSQKK KKSTAPMGNG QPVNQLCQLL GAMIKSQRQQ PRGGQAKKKK PEKPHFPLAA
EDDIRHHLTQ TERSLCLQSI QTAFNQGAGT ASLSSSGKVS FQVEFMLPVA HTVRLIRVTS
TSASQGAS
