NCAP_RABVN
ID NCAP_RABVN Reviewed; 450 AA.
AC O55611; Q75T12; Q85412;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 23-FEB-2022, entry version 79.
DE RecName: Full=Nucleoprotein;
DE Short=NP;
DE AltName: Full=Nucleocapsid protein;
DE Short=Protein N;
GN Name=N;
OS Rabies virus (strain Nishigahara RCEH) (RABV).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae;
OC Lyssavirus.
OX NCBI_TaxID=11298;
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=40674; Mammalia.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=RC-HL;
RX PubMed=8073640; DOI=10.1007/bf01703607;
RA Goto H., Minamoto N., Ito H., Sugiyama M., Kinjo T., Mannen K., Mifune K.,
RA Kawai A.;
RT "Nucleotide sequence of the nucleoprotein gene of the RC.HL strain of
RT rabies virus, a seed strain used for animal vaccine production in Japan.";
RL Virus Genes 8:91-97(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Minamoto N.;
RT "Rabies virus mRNA for nucleoprotein, strain:Nishigahara.";
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Nishigahara, and RC-HL;
RX PubMed=11270607; DOI=10.1111/j.1348-0421.2001.tb01274.x;
RA Ito N., Kakemizu M., Ito K.A., Yamamoto A., Yoshida Y., Sugiyama M.,
RA Minamoto N.;
RT "A comparison of complete genome sequences of the attenuated RC-HL strain
RT of rabies virus used for production of animal vaccine in Japan, and the
RT parental Nishigahara strain.";
RL Microbiol. Immunol. 45:51-58(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Ni-CE;
RX PubMed=17010466; DOI=10.1016/j.virusres.2006.08.011;
RA Shimizu K., Ito N., Mita T., Yamada K., Hosokawa-Muto J., Sugiyama M.,
RA Minamoto N.;
RT "Involvement of nucleoprotein, phosphoprotein, and matrix protein genes of
RT rabies virus in virulence for adult mice.";
RL Virus Res. 123:154-160(2007).
CC -!- FUNCTION: Encapsidates the genome in a ratio of one protein N per nine
CC ribonucleotides, protecting it from nucleases. If expressed without
CC protein P it binds non-specifically RNA and therefore can bind it's own
CC mRNA. Interaction with protein P abolishes any non-specific RNA
CC binding, and prevents phosphorylation. The soluble N-P complex
CC encapsidates specifically the genomic RNA, with protein N protecting
CC the genome like a pearl necklace. The encapsidated genomic RNA is
CC termed the nucleocapsid (NC) and serves as template for viral
CC transcription and replication. Protein N binds protein P in the NC
CC through a different interaction, and can be phosphorylated. Subsequent
CC viral replication is dependent on intracellular concentration of newly
CC synthesized protein N. During replication, encapsidation by protein N
CC is coupled to RNA synthesis and all replicative products are resistant
CC to nucleases (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC genomic RNA. In nucleocapsid, binds protein P and thereby positions the
CC polymerase on the template. Protein P acts as a chaperone on free
CC protein N to prevent it from aggregation before encapsidating genomic
CC RNA (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm {ECO:0000250}.
CC -!- PTM: Phosphorylated by host CK2. Unphosphorylated protein N seems to
CC have a better affinity for leader viral promoter encapsidation.
CC Phosphorylation of protein N in ribonucleocapsid may stabilize the
CC interaction with protein P, thereby playing an important role in viral
CC transcription/replication (By similarity). {ECO:0000250}.
CC -!- MISCELLANEOUS: Displays a superantigen activity in human and mouse,
CC activating mostly V-beta-8 subtypes of T-cell receptor. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the lyssavirus nucleocapsid protein family.
CC {ECO:0000305}.
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DR EMBL; D16331; BAA03838.1; -; Genomic_RNA.
DR EMBL; AB010494; BAA24618.1; -; mRNA.
DR EMBL; AB009663; BAA24083.1; -; Genomic_RNA.
DR EMBL; AB044824; BAA96802.1; -; Genomic_RNA.
DR EMBL; AB128149; BAD04910.1; -; Genomic_RNA.
DR PIR; A58460; A58460.
DR SMR; O55611; -.
DR Proteomes; UP000006366; Genome.
DR Proteomes; UP000007309; Genome.
DR Proteomes; UP000007310; Genome.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3570.10; -; 1.
DR Gene3D; 1.10.3610.10; -; 1.
DR InterPro; IPR000448; Rhabdo_ncapsid.
DR InterPro; IPR023331; Rhabdovirus_ncapsid_C.
DR InterPro; IPR023330; Rhabdovirus_ncapsid_N.
DR InterPro; IPR035961; Rhabdovirus_nucleoprotein-like.
DR Pfam; PF00945; Rhabdo_ncap; 1.
DR SUPFAM; SSF140809; SSF140809; 1.
PE 2: Evidence at transcript level;
KW Capsid protein; Helical capsid protein; Host cytoplasm; Phosphoprotein;
KW Ribonucleoprotein; RNA-binding; Superantigen; Viral nucleoprotein; Virion.
FT CHAIN 1..450
FT /note="Nucleoprotein"
FT /id="PRO_0000295212"
FT MOD_RES 389
FT /note="Phosphoserine; by host CK2"
FT /evidence="ECO:0000250"
FT VARIANT 10
FT /note="S -> A (in strain: RC-HL)"
FT VARIANT 128
FT /note="M -> L (in strain: RC-HL)"
FT VARIANT 257
FT /note="I -> T (in strain: RC-HL)"
FT VARIANT 273
FT /note="F -> L (in strain: Ni-CE)"
FT VARIANT 394..396
FT /note="YFS -> HFP (in strain: RC-HL)"
FT VARIANT 394..395
FT /note="YF -> HL (in strain: Ni-CE)"
SQ SEQUENCE 450 AA; 50720 MW; 7DAFB96AA7055A2C CRC64;
MDADRIVFRS NNQVVSLRPE IIADQYEYKY PAIKDLKKPC ITLGKAPDLN KAYKSVLSGM
NAAKLDPDDV CSYLAAAMQF FEGTCPEDWT SYGILIARKG DKITPNSLVE IKRNDVEGNW
ALTGGMEMTR DPTVSEHASL VGLLLSLYRL SKISGQNTGN YKTNIADRIE QIFETAPFVK
IVEHHTLMTT HKMCANWSTI PNFRFLAGTY DMFFSRIEHL YSAIRVGTVV TAYEDCSGLV
SFTGFIKQIN LTAREAILYF FHKNFEEEIR RMFEPGQETA VPHSYFIHFR SLGLSGKSPY
SSNAVGHVFN LIHFVGCYMG QIRSLNATVI AACAPHEMSV LGGYLGEEFF GRGTFERRFF
RDEKELQEYE AAELTKTDVA LADDGTVDSD DEDYFSGEAR GPEAVYARIM MNGGRLKRSH
IRRYVSVSSN HQARPNSFAE FLNKTYSSDS
