ID   NCAP_RYSV               Reviewed;         521 AA.
AC   Q86523;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   02-JUN-2021, entry version 65.
DE   RecName: Full=Nucleoprotein;
DE            Short=NP;
DE   AltName: Full=Nucleocapsid protein;
DE            Short=Protein N;
GN   Name=N;
OS   Rice yellow stunt virus (RYSV) (Rice transitory yellowing virus).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Rhabdoviridae; Nucleorhabdovirus.
OX   NCBI_TaxID=59380;
OH   NCBI_TaxID=4530; Oryza sativa (Rice).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8091667; DOI=10.1006/viro.1994.1541;
RA   Fang R.X., Wang Q., Xu B.Y., Pang Z., Zhu H.T., Mang K.Q., Gao D.M.,
RA   Qin W.S., Chua N.H.;
RT   "Structure of the nucleocapsid protein gene of rice yellow stunt
RT   rhabdovirus.";
RL   Virology 204:367-375(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=12867659; DOI=10.1099/vir.0.19195-0;
RA   Huang Y., Zhao H., Luo Z., Chen X., Fang R.X.;
RT   "Novel structure of the genome of Rice yellow stunt virus: identification
RT   of the gene 6-encoded virion protein.";
RL   J. Gen. Virol. 84:2259-2264(2003).
RN   [3]
RP   INTERACTION WITH MOVEMENT PROTEIN.
RX   PubMed=15681413; DOI=10.1128/jvi.79.4.2108-2114.2005;
RA   Huang Y.W., Geng Y.F., Ying X.B., Chen X.Y., Fang R.X.;
RT   "Identification of a movement protein of rice yellow stunt rhabdovirus.";
RL   J. Virol. 79:2108-2114(2005).
CC   -!- FUNCTION: Encapsidates the genome, protecting it from nucleases. If
CC       expressed without protein P it binds non-specifically RNA and therefore
CC       can bind it's own mRNA. Interaction with protein P abolishes any non-
CC       specific RNA binding, and prevents phosphorylation. The soluble N-P
CC       complex encapsidates specifically the genomic RNA, with protein N
CC       protecting the genome like a pearl necklace. The encapsidated genomic
CC       RNA is termed the nucleocapsid (NC) and serves as template for viral
CC       transcription and replication. Protein N binds protein P in the NC
CC       through a different interaction, and can be phosphorylated. Subsequent
CC       viral replication is dependent on intracellular concentration of newly
CC       synthesized protein N. During replication, encapsidation by protein N
CC       is coupled to RNA synthesis and all replicative products are resistant
CC       to nucleases (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC       genomic RNA. Interacts with movement protein 3 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the nucleorhabdovirus nucleocapsid protein
CC       family. {ECO:0000305}.
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DR   EMBL; X75534; CAA53224.1; -; mRNA.
DR   EMBL; AB011257; BAA25154.1; -; Genomic_RNA.
DR   RefSeq; NP_620496.1; NC_003746.1.
DR   GeneID; 944310; -.
DR   KEGG; vg:944310; -.
DR   Proteomes; UP000002325; Genome.
DR   GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR004902; Rhabdo_ncap_2.
DR   Pfam; PF03216; Rhabdo_ncap_2; 1.
PE   1: Evidence at protein level;
KW   Capsid protein; Helical capsid protein; Host cytoplasm; Reference proteome;
KW   Ribonucleoprotein; RNA-binding; Superantigen; Viral nucleoprotein; Virion.
FT   CHAIN           1..521
FT                   /note="Nucleoprotein"
FT                   /id="PRO_0000299202"
FT   REGION          406..505
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        413..443
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   521 AA;  58402 MW;  42517E2FFD107783 CRC64;
     MANDNVSDYA NAAPFARFAN LQNRETLNPI GNEAKEIPYN RDQYLTWLAE GKLFQIGALT
     DAEIVAAWTT IKTAMGNNTF SETHMRSIVK IACNLRGITP GSTPLLVTYN PPQSATWAPA
     PSTDAIYSGT PVAGVIIPQN TGAGGEDTET EASKARAIAF ICCYLLRFIV KTEEHLTNSL
     GNLKLQYSRL YSAQSATLSN WNPSNTWASR VKLGFDTYLT LRATVAYNIA SADALLVPEN
     VNYGLCRMLV FQHLELSGLQ LYKMAMTLIA HFKLIEPNKF LSWIYDPLSE ASIDQIYKIA
     VNYDNVNSKT HKHWKYAKLA RGQYWLNTTV KRNQFLAYIL ADLELKYGLA GKSDYSSPKR
     MKALSGMPVE RMTEAETISK AVEQMYTAIE SAKRVDAGAA YRLAKKLGPP RANAHSRRKE
     PNNSRQHRDK QPNSKQQGRD KRNKHQVLGR HSKPQAQGPP NKQQDLGPHN NKPKEASRPP
     QQDRQQLAQP WRLTRRQRGA RGHRTQTLSG MFCKGTCQCI Q