ID   NCAP_SENDE              Reviewed;         517 AA.
AC   P04857;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   02-DEC-2020, entry version 78.
DE   RecName: Full=Nucleoprotein;
DE   AltName: Full=Nucleocapsid protein;
DE            Short=NP;
DE            Short=Protein N;
GN   Name=N; Synonyms=NP;
OS   Sendai virus (strain Enders) (SeV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae;
OC   Respirovirus.
OX   NCBI_TaxID=11194;
OH   NCBI_TaxID=10144; Cavia cutleri (Guinea pig).
OH   NCBI_TaxID=36483; Cricetidae sp. (Hamster).
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
OH   NCBI_TaxID=10116; Rattus norvegicus (Rat).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=6203217; DOI=10.1016/0042-6822(84)90137-5;
RA   Morgan E.M., Re G.G., Kingsbury D.W.;
RT   "Complete sequence of the Sendai virus NP gene from a cloned insert.";
RL   Virology 135:279-287(1984).
RN   [2]
RP   STRUCTURE BY ELECTRON MICROSCOPY (24 ANGSTROMS) OF THE NUCLEOCAPSID.
RX   PubMed=2539515; DOI=10.1128/jvi.63.5.2233-2243.1989;
RA   Egelman E.H., Wu S.-S., Amrein M., Portner A., Murti G.;
RT   "The Sendai virus nucleocapsid exists in at least four different helical
RT   states.";
RL   J. Virol. 63:2233-2243(1989).
CC   -!- FUNCTION: Encapsidates the genome in a ratio of one N per six
CC       ribonucleotides, protecting it from nucleases. The nucleocapsid (NC)
CC       has a helical structure with 13.07 N per turn. The encapsidated genomic
CC       RNA is termed the NC and serves as template for transcription and
CC       replication. Replication is dependent on intracellular concentration of
CC       newly synthesized N, termed N(0), which corresponds to the protein not
CC       associated with RNA. In contrast, when associated with RNA, it is
CC       termed N. During replication, encapsidation by N(0) is coupled to RNA
CC       synthesis and all replicative products are resistant to nucleases.
CC   -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC       genomic RNA. N in nucleocapsid binds the P protein and thereby
CC       positions the polymerase on the template. Interaction of N(0) with the
CC       P protein prevents the uncontrolled aggregation of N(0) (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host cytoplasm.
CC   -!- DOMAIN: There are two distinct binding regions to the P protein, one
CC       used by N(0) and the other by N in nucleocapsid. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Most abundant protein in the virion. There are 2564
CC       molecules per encapsidated genome (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the paramyxoviruses nucleocapsid family.
CC       {ECO:0000305}.
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DR   PDB; 1FZK; X-ray; 1.70 A; P=324-332.
DR   PDB; 1FZO; X-ray; 1.80 A; P=324-332.
DR   PDB; 1KPV; X-ray; 1.71 A; P=324-332.
DR   PDB; 1QLF; X-ray; 2.65 A; C=324-332.
DR   PDB; 2VAB; X-ray; 2.50 A; P=324-332.
DR   PDBsum; 1FZK; -.
DR   PDBsum; 1FZO; -.
DR   PDBsum; 1KPV; -.
DR   PDBsum; 1QLF; -.
DR   PDBsum; 2VAB; -.
DR   BMRB; P04857; -.
DR   SMR; P04857; -.
DR   EvolutionaryTrace; P04857; -.
DR   GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   InterPro; IPR002021; Paramyx_ncap.
DR   Pfam; PF00973; Paramyxo_ncap; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Capsid protein; Helical capsid protein; Host cytoplasm;
KW   Ribonucleoprotein; RNA-binding; Viral nucleoprotein; Virion.
FT   CHAIN           1..517
FT                   /note="Nucleoprotein"
FT                   /id="PRO_0000142680"
FT   REGION          462..471
FT                   /note="Binding of N in nucleoprotein to P protein"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   517 AA;  56733 MW;  6DF90573BF27B808 CRC64;
     MAGLLSTFDT FSSRRSESIN KSGGGAVIPG QRSTVSVFVL GPSVTDDADK LFIATTFLAH
     SLDTDKQHSQ RGGFLVSLLA MAYSSPELYL TTNGVNADVK YVIYNIEKDP KRTKTDGFIV
     KTRDMEYERT TEWLFGPMVN KSPLFQGQRD AADPDTLLQT YGYPACLGAI IVQVWIVLVK
     AITSSAGLRK GFFNRLEAFR QDGTVKGALV FTGETVEGIG SVMRSQQSLV SLMVETLVTM
     NTARSDLTTL EKNIQIVGNY IRDAGLASFM NTIKYGVETK MAALTLSNLR PDINKLRSLI
     DTYLSKGPRA PFICILKDPV HGEFAPGNYP ALWSYAMGVA VVQNKAMQQY VTGRTYLDME
     MFLLGQAVAK DAESKISSAL EDELGVTDTA KERLRHHLAN LSGGDGAYHK PTGGGAIEVA
     LDNADIDLET EAHADQDARG WGGESGERWA RQVSGGHFVT LHGAERLEEE TNDEDVSDIE
     RRIAMRLAER RQGILQPMEM KAAITVWITT KMTMPQQ