ID   NCAP_SENDH              Reviewed;         169 AA.
AC   P14155;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   23-FEB-2022, entry version 67.
DE   RecName: Full=Nucleoprotein;
DE   AltName: Full=Nucleocapsid protein;
DE            Short=NP;
DE            Short=Protein N;
DE   Flags: Fragment;
GN   Name=N; Synonyms=NP;
OS   Sendai virus (strain Harris) (SeV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae;
OC   Respirovirus.
OX   NCBI_TaxID=11196;
OH   NCBI_TaxID=10144; Cavia cutleri (Guinea pig).
OH   NCBI_TaxID=36483; Cricetidae sp. (Hamster).
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
OH   NCBI_TaxID=10116; Rattus norvegicus (Rat).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=6323622; DOI=10.1099/0022-1317-65-4-769;
RA   Blumberg B.M., Giorgi C., Rose K., Kolakofsky D.;
RT   "Preparation and analysis of the nucleocapsid proteins of vesicular
RT   stomatitis virus and sendai virus, and analysis of the sendai virus leader-
RT   NP gene region.";
RL   J. Gen. Virol. 65:769-779(1984).
RN   [2]
RP   RNA-BINDING.
RX   PubMed=8392616; DOI=10.1128/jvi.67.8.4822-4830.1993;
RA   Calain P., Roux L.;
RT   "The rule of six, a basic feature for efficient replication of Sendai virus
RT   defective interfering RNA.";
RL   J. Virol. 67:4822-4830(1993).
RN   [3]
RP   INTERACTION OF N(0) WITH P PROTEIN.
RX   PubMed=7815552; DOI=10.1128/jvi.69.2.849-855.1995;
RA   Curran J., Marq J.-B., Kolakofsky D.;
RT   "An N-terminal domain of the Sendai paramyxovirus P protein acts as a
RT   chaperone for the NP protein during the nascent chain assembly step of
RT   genome replication.";
RL   J. Virol. 69:849-855(1995).
CC   -!- FUNCTION: Encapsidates the genome in a ratio of one N per six
CC       ribonucleotides, protecting it from nucleases. The nucleocapsid (NC)
CC       has a helical structure with 13.07 N per turn. The encapsidated genomic
CC       RNA is termed the NC and serves as template for transcription and
CC       replication. Replication is dependent on intracellular concentration of
CC       newly synthesized N, termed N(0), which corresponds to the protein not
CC       associated with RNA. In contrast, when associated with RNA, it is
CC       termed N. During replication, encapsidation by N(0) is coupled to RNA
CC       synthesis and all replicative products are resistant to nucleases.
CC   -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC       genomic RNA. N in nucleocapsid binds the P protein and thereby
CC       positions the polymerase on the template. Interaction of N(0) with the
CC       P protein prevents the uncontrolled aggregation of N(0).
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host cytoplasm.
CC   -!- DOMAIN: There are two distinct binding regions to the P protein, one
CC       used by N(0) and the other by N in nucleocapsid. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Most abundant protein in the virion. There are 2564
CC       molecules per encapsidated genome.
CC   -!- SIMILARITY: Belongs to the paramyxoviruses nucleocapsid family.
CC       {ECO:0000305}.
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DR   EMBL; M29343; AAA47812.1; -; Genomic_RNA.
DR   SMR; P14155; -.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0032991; C:protein-containing complex; IMP:CAFA.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR   GO; GO:0019904; F:protein domain specific binding; IPI:CAFA.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0039689; P:negative stranded viral RNA replication; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Host cytoplasm; Ribonucleoprotein; RNA-binding; Viral nucleoprotein;
KW   Virion.
FT   CHAIN           1..>169
FT                   /note="Nucleoprotein"
FT                   /id="PRO_0000142682"
FT   NON_TER         169
SQ   SEQUENCE   169 AA;  18589 MW;  D2CC4E1863E1A5D2 CRC64;
     MAGLLSTFDT FSSRRSESIN KSGGGAVIPG QKSTVSVFVL GPSVTDDADK LFIATTFLAH
     SLNTDKQHSQ RGGFLVSLLA MAYSSPELYL TTNGVNADVK YVIYNIEKDP KRTKTDRFIV
     KTRDMEYERT TEWLFGPMVN KSPLFQGQRD AADPDTLLQT YGYPACLGA