NCAP_SENDZ
ID NCAP_SENDZ Reviewed; 517 AA.
AC P04858; P27563; Q88268;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 07-OCT-2020, entry version 80.
DE RecName: Full=Nucleoprotein;
DE AltName: Full=Nucleocapsid protein;
DE Short=NP;
DE Short=Protein N;
GN Name=N; Synonyms=NP;
OS Sendai virus (strain Z) (SeV) (Sendai virus (strain HVJ)).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae;
OC Respirovirus.
OX NCBI_TaxID=11198;
OH NCBI_TaxID=10144; Cavia cutleri (Guinea pig).
OH NCBI_TaxID=36483; Cricetidae sp. (Hamster).
OH NCBI_TaxID=10090; Mus musculus (Mouse).
OH NCBI_TaxID=10116; Rattus norvegicus (Rat).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=6316257; DOI=10.1093/nar/11.21.7317;
RA Shioda T., Hidaka Y., Kanda T., Shibuta H., Nomoto A., Iwasaki K.;
RT "Sequence of 3,687 nucleotides from the 3' end of Sendai virus genome RNA
RT and the predicted amino acid sequences of viral NP, P and C proteins.";
RL Nucleic Acids Res. 11:7317-7330(1983).
RN [2]
RP SEQUENCE REVISION.
RA Shibuta H.;
RL Submitted (FEB-1985) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Mutant F1-R, and Mutant ts-f1;
RX PubMed=2161155; DOI=10.1016/0042-6822(90)90040-x;
RA Middleton Y., Tashiro M., Thai T., Oh J., Seymour J., Pritzer E.,
RA Klenk H.-D., Rott R., Seto J.T.;
RT "Nucleotide sequence analyses of the genes encoding the HN, M, NP, P, and L
RT proteins of two host range mutants of Sendai virus.";
RL Virology 176:656-657(1990).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Mutant F1-R / T-5 revertant, and Mutant F1-R / T-7 revertant;
RX PubMed=1651590; DOI=10.1016/0042-6822(91)90839-4;
RA Tashiro M., James I., Karri S., Wahn K., Tobita K., Klenk H.-D., Rott R.,
RA Seto J.T.;
RT "Pneumotropic revertants derived from a pantropic mutant, F1-R, of Sendai
RT virus.";
RL Virology 184:227-234(1991).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 324-332 COMPLEXED WITH MHC CLASS I
RP H-2DB.
RX PubMed=10023771; DOI=10.1016/s1074-7613(00)80007-2;
RA Glithero A., Tormo J., Haurum J.S., Arsequell G., Valencia G., Edwards J.,
RA Springer S., Townsend A., Pao Y.L., Wormald M., Dwek R.A., Jones E.Y.,
RA Elliott T.;
RT "Crystal structures of two H-2Db/glycopeptide complexes suggest a molecular
RT basis for CTL cross-reactivity.";
RL Immunity 10:63-74(1999).
CC -!- FUNCTION: Encapsidates the genome in a ratio of one N per six
CC ribonucleotides, protecting it from nucleases. The nucleocapsid (NC)
CC has a helical structure with 13.07 N per turn. The encapsidated genomic
CC RNA is termed the NC and serves as template for transcription and
CC replication. Replication is dependent on intracellular concentration of
CC newly synthesized N, termed N(0), which corresponds to the protein not
CC associated with RNA. In contrast, when associated with RNA, it is
CC termed N. During replication, encapsidation by N(0) is coupled to RNA
CC synthesis and all replicative products are resistant to nucleases.
CC -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC genomic RNA. N in nucleocapsid binds the P protein and thereby
CC positions the polymerase on the template. Interaction of N(0) with the
CC P protein prevents the uncontrolled aggregation of N(0) (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host cytoplasm.
CC -!- DOMAIN: There are two distinct binding regions to the P protein, one
CC used by N(0) and the other by N in nucleocapsid. {ECO:0000250}.
CC -!- MISCELLANEOUS: Most abundant protein in the virion. There are 2564
CC molecules per encapsidated genome (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the paramyxoviruses nucleocapsid family.
CC {ECO:0000305}.
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DR EMBL; X00087; CAA24945.1; -; Genomic_RNA.
DR EMBL; M30202; AAB06278.1; -; Genomic_RNA.
DR EMBL; M30203; AAB06284.1; -; Genomic_RNA.
DR EMBL; M30204; AAB06196.1; -; Genomic_RNA.
DR EMBL; M69046; AAB06290.1; -; Genomic_RNA.
DR EMBL; M55565; AAB06297.1; -; Genomic_RNA.
DR PIR; A04029; VHNZSV.
DR PDB; 1CE6; X-ray; 2.90 A; C=324-332.
DR PDBsum; 1CE6; -.
DR BMRB; P04858; -.
DR SMR; P04858; -.
DR EvolutionaryTrace; P04858; -.
DR Proteomes; UP000006560; Genome.
DR Proteomes; UP000110830; Genome.
DR Proteomes; UP000163956; Genome.
DR Proteomes; UP000169749; Genome.
DR Proteomes; UP000181310; Genome.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR InterPro; IPR002021; Paramyx_ncap.
DR Pfam; PF00973; Paramyxo_ncap; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Capsid protein; Helical capsid protein; Host cytoplasm;
KW Reference proteome; Ribonucleoprotein; RNA-binding; Viral nucleoprotein;
KW Virion.
FT CHAIN 1..517
FT /note="Nucleoprotein"
FT /id="PRO_0000142684"
FT REGION 462..471
FT /note="Binding of N in nucleoprotein to P protein"
FT /evidence="ECO:0000250"
FT VARIANT 24
FT /note="R -> G (in strain: Mutant F1-R, Mutant ts-f1, Mutant
FT F1-R / T-5 revertant and Mutant F1-R / T-7 revertant)"
FT VARIANT 42
FT /note="L -> P (in strain: Mutant F1-R / T-5 revertant and
FT Mutant F1-R / T-7 revertant)"
FT VARIANT 67
FT /note="R -> Q (in strain: Mutant F1-R, Mutant ts-f1, Mutant
FT F1-R / T-5 revertant and Mutant F1-R / T-7 revertant)"
FT VARIANT 377
FT /note="S -> T (in strain: Mutant F1-R, Mutant ts-f1, Mutant
FT F1-R / T-5 revertant and Mutant F1-R / T-7 revertant)"
FT VARIANT 388..389
FT /note="EA -> DT (in strain: Mutant F1-R, Mutant ts-f1,
FT Mutant F1-R / T-5 revertant and Mutant F1-R / T-7
FT revertant)"
FT VARIANT 400
FT /note="S -> N (in strain: Mutant F1-R, Mutant ts-f1, Mutant
FT F1-R / T-5 revertant and Mutant F1-R / T-7 revertant)"
FT VARIANT 405
FT /note="N -> D (in strain: Mutant F1-R, Mutant ts-f1, Mutant
FT F1-R / T-5 revertant and Mutant F1-R / T-7 revertant)"
FT VARIANT 489
FT /note="E -> G (in strain: Mutant F1-R and Mutant F1-R / T-7
FT revertant)"
FT VARIANT 494..517
FT /note="ILQPMEMKAAITVSIMTKMTIPQQ -> DSATHGDEGRNNGVDHDEDDDTAA
FT VAGVGGI (in strain: Mutant F1-R, Mutant ts-f1, Mutant F1-R
FT / T-5 revertant and Mutant F1-R / T-7 revertant)"
SQ SEQUENCE 517 AA; 56762 MW; AD25055F8A624973 CRC64;
MAGLLSTFDT FSSRRSESIN KSGRGAVIPG QRSTVSVFVL GLSVTDDADK LFIATTFLAH
SLDTDKRHSQ RGGFLVSLLA MAYSSPELYL TTNGVNADVK YVIYNIEKDP KRTKTDGFIV
KTRDMEYERT TEWLFGPMVN KSPLFQGQRD AADPDTLLQI YGYPACLGAI IVQVWIVLVK
AITSSAGLRK GFFNRLEAFR QDGTVKGALV FTGETVEGIG SVMRSQQSLV SLMVETLVTM
NTARSDLTTL EKNIQIVGNY IRDAGLASFM NTIKYGVETK MAALTLSNLR PDINKLRSLI
DTYLSKGPRA PFICILKDPV HGEFAPGNYP ALWSYAMGVA VVQNKAMQQY VTGRTYLDME
MFLLGQAVAK DAESKISSAL EDELGVTEAA KGRLRHHLAS LSGGNGAYRK PTGGGAIEVA
LDNADIDLET KAHADQDARG WGGDSGERWA RQVSGGHFVT LHGAERLEEE TNDEDVSDIE
RRIAMRLAER RQEILQPMEM KAAITVSIMT KMTIPQQ
