NCAP_SFTS
ID NCAP_SFTS Reviewed; 245 AA.
AC I6WJ72;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 1.
DT 03-AUG-2022, entry version 38.
DE RecName: Full=Nucleoprotein;
DE AltName: Full=Nucleocapsid protein;
DE Short=Protein N;
GN Name=NP;
OS Dabie bandavirus (Severe fever with thrombocytopenia virus) (Huaiyangshan
OS banyangvirus).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Ellioviricetes; Bunyavirales; Phenuiviridae; Bandavirus; Dabie bandavirus.
OX NCBI_TaxID=1003835;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Isolate JS2010-018;
RA Shan J., Qi X., Cui L., Zhao K., Guo X., Wu T., Peng H., Chen Y., Tang F.,
RA Zhou M., Wang H.;
RT "Genetic characteristics of novel Phlebovirus related to epidemic fever
RT with thrombocytopenia syndrome in Jiangsu, China.";
RL Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP SUBCELLULAR LOCATION.
RC STRAIN=YG1;
RX PubMed=29467349; DOI=10.2220/biomedres.39.27;
RA Lundu T., Tsuda Y., Ito R., Shimizu K., Kobayashi S., Yoshii K.,
RA Yoshimatsu K., Arikawa J., Kariwa H.;
RT "Targeting of severe fever with thrombocytopenia syndrome virus structural
RT proteins to the ERGIC (endoplasmic reticulum Golgi intermediate
RT compartment) and Golgi complex.";
RL Biomed. Res. 39:27-38(2018).
RN [3]
RP SUBCELLULAR LOCATION, AND RNA-BINDING.
RC STRAIN=YG1;
RX PubMed=34836987; DOI=10.1038/s41598-021-01985-x;
RA Lokupathirage S.M.W., Tsuda Y., Ikegame K., Noda K., Muthusinghe D.S.,
RA Kozawa F., Manzoor R., Shimizu K., Yoshimatsu K.;
RT "Subcellular localization of nucleocapsid protein of SFTSV and its assembly
RT into the ribonucleoprotein complex with L protein and viral RNA.";
RL Sci. Rep. 11:22977-22977(2021).
RN [4] {ECO:0007744|PDB:4J4R, ECO:0007744|PDB:4J4S}
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH SURAMIN, AND
RP SUBUNIT.
RX PubMed=23576501; DOI=10.1128/jvi.00672-13;
RA Jiao L., Ouyang S., Liang M., Niu F., Shaw N., Wu W., Ding W., Jin C.,
RA Peng Y., Zhu Y., Zhang F., Wang T., Li C., Zuo X., Luan C.H., Li D.,
RA Liu Z.J.;
RT "Structure of severe fever with thrombocytopenia syndrome virus
RT nucleocapsid protein in complex with suramin reveals therapeutic
RT potential.";
RL J. Virol. 87:6829-6839(2013).
CC -!- FUNCTION: Encapsidates the genomic RNA, protecting it from nucleases.
CC Displays high affinity for single-stranded nucleic acid. The
CC encapsidated genomic RNA is termed the nucleocapsid (NC) or
CC ribonucleoprotein (By similarity). The ribonucleoprotein has a non-
CC helical structure (By similarity). Serves as template for viral
CC transcription and replication. After replication, the nucleocapsid is
CC recruited to the host Golgi apparatus by glycoprotein Gn for packaging
CC into virus particles (By similarity). {ECO:0000250|UniProtKB:D3K5I7,
CC ECO:0000250|UniProtKB:F1BA48, ECO:0000250|UniProtKB:P21700}.
CC -!- SUBUNIT: Homodimer (By similarity). Homohexamer; ring-shaped, necessary
CC to form the nucleocapsid (PubMed:23576501). Homopentamers; opened
CC pentamers in solution (By similarity). Binds to viral genomic RNA (By
CC similarity). Interacts with glycoprotein Gn; this interaction allows
CC packaging of nucleocapsids into virions (By similarity).
CC {ECO:0000250|UniProtKB:D3K5I7, ECO:0000250|UniProtKB:P21700,
CC ECO:0000250|UniProtKB:P21701, ECO:0000269|PubMed:23576501}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000250|UniProtKB:D3K5I7}. Host
CC cytoplasm {ECO:0000250|UniProtKB:D3K5I7}. Host nucleus
CC {ECO:0000250|UniProtKB:D3K5I7}. Host endoplasmic reticulum-Golgi
CC intermediate compartment {ECO:0000269|PubMed:29467349,
CC ECO:0000269|PubMed:34836987}. Host Golgi apparatus
CC {ECO:0000269|PubMed:29467349, ECO:0000269|PubMed:34836987}.
CC -!- MISCELLANEOUS: Binds suramin, which inhibits the replication of SFTSV
CC in vitro. {ECO:0000269|PubMed:23576501}.
CC -!- SIMILARITY: Belongs to the phlebovirus nucleocapsid protein family.
CC {ECO:0000305}.
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DR EMBL; JQ317177; AFN26700.1; -; Genomic_RNA.
DR PDB; 4J4R; X-ray; 1.90 A; A=1-245.
DR PDB; 4J4S; X-ray; 2.44 A; A/B/C/D=1-245.
DR PDB; 4J4U; X-ray; 2.80 A; A/B/C/D/E=1-245.
DR PDB; 4J4V; X-ray; 2.30 A; A/B/C/D/E=1-245.
DR PDBsum; 4J4R; -.
DR PDBsum; 4J4S; -.
DR PDBsum; 4J4U; -.
DR PDBsum; 4J4V; -.
DR SMR; I6WJ72; -.
DR Proteomes; UP000144974; Genome.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-UniRule.
DR GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR InterPro; IPR009522; Capsid_Phlebovir/Tenuivir.
DR InterPro; IPR015971; Nucleocapsid_Phlebovirus.
DR Pfam; PF05733; Tenui_N; 1.
DR PIRSF; PIRSF003953; N_PhelboV; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Capsid protein; Host cytoplasm; Host Golgi apparatus;
KW Host nucleus; Ribonucleoprotein; RNA-binding; Viral nucleoprotein; Virion.
FT CHAIN 1..245
FT /note="Nucleoprotein"
FT /id="PRO_0000456177"
FT SITE 64
FT /note="RNA-binding"
FT /evidence="ECO:0000250|UniProtKB:F1BA48"
FT SITE 67
FT /note="RNA-binding"
FT /evidence="ECO:0000250|UniProtKB:F1BA48"
FT SITE 70
FT /note="RNA-binding"
FT /evidence="ECO:0000250|UniProtKB:F1BA48"
FT SITE 95
FT /note="RNA-binding"
FT /evidence="ECO:0000250|UniProtKB:F1BA48"
FT SITE 106
FT /note="RNA-binding"
FT /evidence="ECO:0000250|UniProtKB:F1BA48"
FT SITE 184
FT /note="RNA-binding"
FT /evidence="ECO:0000250|UniProtKB:F1BA48"
SQ SEQUENCE 245 AA; 26985 MW; 8CB3FD67684F2F42 CRC64;
MSEWSRIAVE FGEQQLNLTE LEDFARELAY EGLDPALIIK KLKETGGDDW VKDTKFIIVF
ALTRGNKIVK ASGKMSNSGS KRLMALQEKY GLVERAETRL SITPVRVAQS LPTWTCAAAA
ALKEYLPVGP AVMNLKVENY PPEMMCMAFG SLIPTAGVSE ATTKTLMEAY SLWQDAFTKT
INVKMRGASK TEVYNSFRDP LHAAVNSVFF PNDVRVKWLK AKGILGPDGV PSRAAEVAAA
AYRNL
