ID   NCAP_VHSV0              Reviewed;         404 AA.
AC   P24378;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   23-FEB-2022, entry version 68.
DE   RecName: Full=Nucleoprotein;
DE            Short=NP;
DE   AltName: Full=Nucleocapsid protein;
DE            Short=Protein N;
GN   Name=N;
OS   Viral hemorrhagic septicemia virus (strain 07-71) (VHSV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Rhabdoviridae; Gammarhabdovirinae;
OC   Novirhabdovirus.
OX   NCBI_TaxID=11288;
OH   NCBI_TaxID=59291; Coregonus lavaretus (Common whitefish) (Salmo lavaretus).
OH   NCBI_TaxID=8010; Esox lucius (Northern pike).
OH   NCBI_TaxID=8019; Oncorhynchus kisutch (Coho salmon) (Salmo kisutch).
OH   NCBI_TaxID=8022; Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
OH   NCBI_TaxID=74940; Oncorhynchus tshawytscha (Chinook salmon) (Salmo tshawytscha).
OH   NCBI_TaxID=8032; Salmo trutta (Brown trout).
OH   NCBI_TaxID=8040; Salvelinus namaycush (Lake trout) (Salmo namaycush).
OH   NCBI_TaxID=36185; Thymallus thymallus (Grayling) (Salmo thymallus).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2202782; DOI=10.1099/0022-1317-71-8-1669;
RA   Bernard J., Lecocq-Xhonneux F., Rossius M., Thiry M.E., de Kinkelin P.;
RT   "Cloning and sequencing the messenger RNA of the N gene of viral
RT   haemorrhagic septicaemia virus.";
RL   J. Gen. Virol. 71:1669-1674(1990).
CC   -!- FUNCTION: Encapsidates the genome, protecting it from nucleases. If
CC       expressed without protein P it binds non-specifically RNA and therefore
CC       can bind it's own mRNA. Interaction with protein P abolishes any non-
CC       specific RNA binding, and prevents phosphorylation. The soluble N-P
CC       complex encapsidates specifically the genomic RNA, with protein N
CC       protecting the genome like a pearl necklace. The encapsidated genomic
CC       RNA is termed the nucleocapsid (NC) and serves as template for viral
CC       transcription and replication. Protein N binds protein P in the NC
CC       through a different interaction, and can be phosphorylated. Subsequent
CC       viral replication is dependent on intracellular concentration of newly
CC       synthesized protein N. During replication, encapsidation by protein N
CC       is coupled to RNA synthesis and all replicative products are resistant
CC       to nucleases (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC       genomic RNA (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the novirhabdovirus nucleocapsid protein family.
CC       {ECO:0000305}.
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DR   EMBL; D00687; BAA00591.1; -; mRNA.
DR   PIR; A36651; VHVNHS.
DR   GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR004902; Rhabdo_ncap_2.
DR   Pfam; PF03216; Rhabdo_ncap_2; 1.
PE   2: Evidence at transcript level;
KW   Capsid protein; Helical capsid protein; Host cytoplasm; Phosphoprotein;
KW   Ribonucleoprotein; RNA-binding; Viral nucleoprotein; Virion.
FT   CHAIN           1..404
FT                   /note="Nucleoprotein"
FT                   /id="PRO_0000222813"
FT   REGION          358..404
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        358..392
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   404 AA;  44370 MW;  6C7200217ABA33A4 CRC64;
     MEGGIRAAFS GLNDVRIDPT GGEGRVLVPG EVELIVYVGE FGEEDRKVIV DALSALGGPQ
     TVQALSVLLS YVLQGNTQED LETKCKVLTD MGFKVTQAVR ATSIEAGIMM PMRELALTVN
     DDNLMEIVKG TLMTCSLLTK YSVDKMIKYI TKKLGELADT QGVGELQHFT ADKAAIRKLA
     GCVRPGQKIT KALYAFILTE IADPTTQSRV PSMGALRLNG TGMTMIGLFT QAANNLGIAP
     AKLLEDLCME SLVESARRII QLMRQVSEAK SIQERYAIMM SRMLGESYYK SYGLNDNSKI
     SYILSQISGK YAVDSLEGLE GIKVTEKFRE FAELVAEVLV DKYERIGEDS TEVSDVIREA
     ARQHARRTSA KPEPKARNFR SSTGRGREQE TGESDDDDYP EDSD