ID   NCAP_VHSVM              Reviewed;         404 AA.
AC   P27371;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   29-SEP-2021, entry version 66.
DE   RecName: Full=Nucleoprotein;
DE            Short=NP;
DE   AltName: Full=Nucleocapsid protein;
DE            Short=Protein N;
GN   Name=N;
OS   Viral hemorrhagic septicemia virus (strain Makah) (VHSV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Rhabdoviridae; Gammarhabdovirinae;
OC   Novirhabdovirus.
OX   NCBI_TaxID=11289;
OH   NCBI_TaxID=59291; Coregonus lavaretus (Common whitefish) (Salmo lavaretus).
OH   NCBI_TaxID=8010; Esox lucius (Northern pike).
OH   NCBI_TaxID=8019; Oncorhynchus kisutch (Coho salmon) (Salmo kisutch).
OH   NCBI_TaxID=8022; Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
OH   NCBI_TaxID=74940; Oncorhynchus tshawytscha (Chinook salmon) (Salmo tshawytscha).
OH   NCBI_TaxID=8032; Salmo trutta (Brown trout).
OH   NCBI_TaxID=8040; Salvelinus namaycush (Lake trout) (Salmo namaycush).
OH   NCBI_TaxID=36185; Thymallus thymallus (Grayling) (Salmo thymallus).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1634868; DOI=10.1099/0022-1317-73-4-1011;
RA   Bernard J., Bremont M., Winton J.;
RT   "Nucleocapsid gene sequence of a North American isolate of viral
RT   haemorrhagic septicaemia virus, a fish rhabdovirus.";
RL   J. Gen. Virol. 73:1011-1014(1992).
CC   -!- FUNCTION: Encapsidates the genome, protecting it from nucleases. If
CC       expressed without protein P it binds non-specifically RNA and therefore
CC       can bind it's own mRNA. Interaction with protein P abolishes any non-
CC       specific RNA binding, and prevents phosphorylation. The soluble N-P
CC       complex encapsidates specifically the genomic RNA, with protein N
CC       protecting the genome like a pearl necklace. The encapsidated genomic
CC       RNA is termed the nucleocapsid (NC) and serves as template for viral
CC       transcription and replication. Protein N binds protein P in the NC
CC       through a different interaction, and can be phosphorylated. Subsequent
CC       viral replication is dependent on intracellular concentration of newly
CC       synthesized protein N. During replication, encapsidation by protein N
CC       is coupled to RNA synthesis and all replicative products are resistant
CC       to nucleases (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC       genomic RNA (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the novirhabdovirus nucleocapsid protein family.
CC       {ECO:0000305}.
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DR   EMBL; X59241; CAA41930.1; -; mRNA.
DR   PIR; JQ1531; JQ1531.
DR   GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR004902; Rhabdo_ncap_2.
DR   Pfam; PF03216; Rhabdo_ncap_2; 1.
PE   2: Evidence at transcript level;
KW   Capsid protein; Helical capsid protein; Host cytoplasm; Phosphoprotein;
KW   Ribonucleoprotein; RNA-binding; Viral nucleoprotein; Virion.
FT   CHAIN           1..404
FT                   /note="Nucleoprotein"
FT                   /id="PRO_0000222814"
FT   REGION          357..404
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        357..392
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   404 AA;  44066 MW;  54505A345E646DA1 CRC64;
     MEGGIRAAFS GLNDVRIDPT GGEGRVLVPG EVELIVYAGP FGTDDGKVIV DALAALGGPQ
     TVQALSVLLS YVLQGSAQGD LEAKCKILTD MGFKVTQSPR ATGIEAGILM PMRELAQTVN
     NDNLMDIVKG ALMTCSLLDK YSVDKMIKYI TKKLGELGST QGVGELQHLS ADKAAIRKLA
     GCVRPGQKIT KALYAFILTE IADPTTQSRV QSMGALRLNG TGMTMIGLFT QAANNLGIPP
     AKLLEDLCME SLVESARRII QLMRQVSEAR SIQERYAIMM SRMLGESYYK SYRLNDNSKI
     SYILSQISGK YAVDSLEGLE GIKVTEKFRE FTELVAEVLV DKYERIGEDS TEVSDVIREA
     ARQHARKASD KPEPKARNFR SSTGRGKEQE KEESDDDDYP GDSD