ID   NCBP1_ANOGA             Reviewed;         807 AA.
AC   Q7PX35;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 4.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Nuclear cap-binding protein subunit 1;
DE   AltName: Full=80 kDa nuclear cap-binding protein;
DE            Short=CBP80;
DE            Short=NCBP 80 kDa subunit;
GN   Name=Cbp80; ORFNames=AGAP001195;
OS   Anopheles gambiae (African malaria mosquito).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Anophelinae; Anopheles.
OX   NCBI_TaxID=7165;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PEST;
RX   PubMed=12364791; DOI=10.1126/science.1076181;
RA   Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R.,
RA   Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R.,
RA   Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z.,
RA   Kraft C.L., Abril J.F., Anthouard V., Arensburger P., Atkinson P.W.,
RA   Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C.,
RA   Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K.,
RA   Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V.,
RA   Dana A., Delcher A., Dew I., Evans C.A., Flanigan M.,
RA   Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R.,
RA   Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J.,
RA   Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I.,
RA   Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A.,
RA   McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D.,
RA   O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H.,
RA   Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J.,
RA   Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B.,
RA   Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M.,
RA   Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I.,
RA   Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J.,
RA   Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M.,
RA   Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C.,
RA   Collins F.H., Hoffman S.L.;
RT   "The genome sequence of the malaria mosquito Anopheles gambiae.";
RL   Science 298:129-149(2002).
CC   -!- FUNCTION: Component of the cap-binding complex (CBC), which binds
CC       cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in
CC       various processes such as pre-mRNA splicing and RNA-mediated gene
CC       silencing (RNAi). The CBC complex is involved in miRNA-mediated RNA
CC       interference and is required for primary microRNAs (miRNAs) processing.
CC       Also involved in innate immunity via the short interfering RNAs
CC       (siRNAs) processing machinery by restricting the viral RNA production.
CC       In the CBC complex, Cbp80 does not bind directly capped RNAs (m7GpppG-
CC       capped RNA) but is required to stabilize the movement of the N-terminal
CC       loop of Cbp20 and lock the CBC into a high affinity cap-binding state
CC       with the cap structure (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the nuclear cap-binding complex (CBC), a
CC       heterodimer composed of Cbp80 and Cbp20 that interacts with m7GpppG-
CC       capped RNA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NCBP1 family. {ECO:0000305}.
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DR   EMBL; AAAB01008987; EAA01817.4; -; Genomic_DNA.
DR   RefSeq; XP_321964.4; XM_321964.4.
DR   AlphaFoldDB; Q7PX35; -.
DR   SMR; Q7PX35; -.
DR   STRING; 7165.AGAP001195-PA; -.
DR   PaxDb; Q7PX35; -.
DR   PRIDE; Q7PX35; -.
DR   GeneID; 1281974; -.
DR   KEGG; aga:AgaP_AGAP001195; -.
DR   CTD; 1281974; -.
DR   VEuPathDB; VectorBase:AGAP001195; -.
DR   eggNOG; KOG1104; Eukaryota.
DR   HOGENOM; CLU_013207_0_0_1; -.
DR   InParanoid; Q7PX35; -.
DR   OMA; QPFKIPF; -.
DR   OrthoDB; 270650at2759; -.
DR   PhylomeDB; Q7PX35; -.
DR   Proteomes; UP000007062; Chromosome 2R.
DR   GO; GO:0005845; C:mRNA cap binding complex; IBA:GO_Central.
DR   GO; GO:0005846; C:nuclear cap binding complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:0000339; F:RNA cap binding; IBA:GO_Central.
DR   GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR   GO; GO:0006406; P:mRNA export from nucleus; IEA:InterPro.
DR   GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IBA:GO_Central.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR027159; CBP80.
DR   InterPro; IPR015172; MIF4G-like_typ-1.
DR   InterPro; IPR015174; MIF4G-like_typ-2.
DR   InterPro; IPR003890; MIF4G-like_typ-3.
DR   PANTHER; PTHR12412; PTHR12412; 1.
DR   Pfam; PF02854; MIF4G; 1.
DR   Pfam; PF09088; MIF4G_like; 1.
DR   Pfam; PF09090; MIF4G_like_2; 1.
DR   SMART; SM00543; MIF4G; 1.
DR   SUPFAM; SSF48371; SSF48371; 3.
PE   3: Inferred from homology;
KW   mRNA capping; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   RNA-mediated gene silencing.
FT   CHAIN           1..807
FT                   /note="Nuclear cap-binding protein subunit 1"
FT                   /id="PRO_0000385231"
FT   DOMAIN          31..243
FT                   /note="MIF4G"
FT   REGION          669..710
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        686..710
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   807 AA;  94505 MW;  E61C4761130A0C33 CRC64;
     MNRRRAYEDD GDFYGERNKK RRRVSENQEM EERLETLILR VGENSTSSLE SNLEGLVSVL
     ESDLGNFRSK ILRILSDCPI KMPEKCTIYS TMVGLMNAKN YNFGGEFVEY MVKTFKDSLK
     QCQWDAARYA LRFLADLVNC HVISTNSLLQ LLDSMVDAAN EDNVPQVRRD WYVFAVLSTL
     PWVGRELYEK KESALENLLV RIEVFLNKRT KKHHNALRVW SVDAPHPQEE YLDCLWAQIR
     KLRQDNWTEK HIPRPYLAFD SVLCEALQHN IPVIHPPPHQ DSFEYPMPWV VYRMFDYTDC
     PPGPILPGAH SIERFLIEEH LHSIIEMHRW ERKDCAIHLL MLPYKDKIPL EYCIVEVIFA
     ELFHMPTPRY LEICYGSILI ELCKQQPSKM PQVLAQATEI LFMRIDSMNT SCFDRFVNWF
     SYHLSNFQFR WSWDDWDSCL LLENEHPRPK FIQEVLLKCL RFSYHDRFKE MMPEGYAKLI
     PKPPVPHYKY SMEGAASLPG TATAHKLVVA IRQKCNAEDV LNELNDLPNS RDASDTDMAE
     APFNPLKIDV FVQTLLNLGS KSFSHSFAAI SKFHAVFKAL AETEEAQICI LHNMFELWVD
     HQQMMVVIVD KLLKVQIVEC SAVATWVFSK EMVGEFTKMY LWEILHLTIK KMNQHVTKLS
     REMNEAKEKL ARTVESSSSE SEDEAASPNA QKRRKNTEGS GEKPTEEQVE RMEEKLEAAY
     VDQKRLFLII FQRFIMILSE HLVKCDTDGR DYDTDWYRWT IGRLQQVFMM HHEQVQKYSS
     TLESLLFTSD LDPHILDVFH QFTALRA