NCBP1_CHICK
ID NCBP1_CHICK Reviewed; 793 AA.
AC Q5ZJZ6;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Nuclear cap-binding protein subunit 1;
DE AltName: Full=80 kDa nuclear cap-binding protein;
DE Short=CBP80;
DE Short=NCBP 80 kDa subunit;
GN Name=NCBP1; Synonyms=CBP80; ORFNames=RCJMB04_14d7;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=CB; TISSUE=Bursa of Fabricius;
RX PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA Hayashizaki Y., Buerstedde J.-M.;
RT "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT function analysis.";
RL Genome Biol. 6:R6.1-R6.9(2005).
CC -!- FUNCTION: Component of the cap-binding complex (CBC), which binds
CC cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in
CC various processes such as pre-mRNA splicing, translation regulation,
CC nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by
CC microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA
CC export from the nucleus, leading to the recruitment of the mRNA export
CC machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3'
CC direction through the nuclear pore. The CBC complex is also involved in
CC mediating U snRNA and intronless mRNAs export from the nucleus. The CBC
CC complex is essential for a pioneer round of mRNA translation, before
CC steady state translation when the CBC complex is replaced by
CC cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA
CC translation mediated by the CBC complex plays a central role in
CC nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs
CC bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex
CC enhances NMD in mRNAs containing at least one exon-junction complex
CC (EJC), promoting the interaction between UPF1 and UPF2. The CBC complex
CC is also involved in 'failsafe' NMD, which is independent of the EJC
CC complex, while it does not participate in Staufen-mediated mRNA decay
CC (SMD). During cell proliferation, the CBC complex is also involved in
CC microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is
CC required for miRNA-mediated RNA interference. The CBC complex also acts
CC as a negative regulator of PARN, thereby acting as an inhibitor of mRNA
CC deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly
CC capped RNAs (m7GpppG-capped RNA) but is required to stabilize the
CC movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a
CC high affinity cap-binding state with the cap structure. Associates with
CC NCBP3 to form an alternative cap-binding complex (CBC) which plays a
CC key role in mRNA export. The conventional CBC with NCBP2 binds both
CC small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their
CC export from the nucleus whereas the alternative CBC with NCBP3 does not
CC bind snRNA and associates only with mRNA thereby playing a role only in
CC mRNA export (By similarity). {ECO:0000250|UniProtKB:Q09161}.
CC -!- SUBUNIT: Component of the nuclear cap-binding complex (CBC), a
CC heterodimer composed of NCBP1/CBP80 and NCBP2/CBP20 that interacts with
CC m7GpppG-capped RNA. Component of an alternative nuclear cap-binding
CC complex (CBC) composed of NCBP1/CBP80 and NCBP3 (By similarity).
CC {ECO:0000250|UniProtKB:Q09161}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q09161}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q09161}.
CC -!- SIMILARITY: Belongs to the NCBP1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ720288; CAG31947.1; -; mRNA.
DR RefSeq; NP_001026611.1; NM_001031440.1.
DR AlphaFoldDB; Q5ZJZ6; -.
DR SMR; Q5ZJZ6; -.
DR STRING; 9031.ENSGALP00000003250; -.
DR PaxDb; Q5ZJZ6; -.
DR PRIDE; Q5ZJZ6; -.
DR Ensembl; ENSGALT00000003255; ENSGALP00000003250; ENSGALG00000002087.
DR GeneID; 427379; -.
DR KEGG; gga:427379; -.
DR CTD; 4686; -.
DR VEuPathDB; HostDB:geneid_427379; -.
DR eggNOG; KOG1104; Eukaryota.
DR GeneTree; ENSGT00390000001733; -.
DR HOGENOM; CLU_013207_0_0_1; -.
DR InParanoid; Q5ZJZ6; -.
DR OMA; QPFKIPF; -.
DR OrthoDB; 270650at2759; -.
DR PhylomeDB; Q5ZJZ6; -.
DR Reactome; R-GGA-111367; SLBP independent Processing of Histone Pre-mRNAs.
DR Reactome; R-GGA-113418; Formation of the Early Elongation Complex.
DR Reactome; R-GGA-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-GGA-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-GGA-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-GGA-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-GGA-6803529; FGFR2 alternative splicing.
DR Reactome; R-GGA-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-GGA-72086; mRNA Capping.
DR Reactome; R-GGA-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-GGA-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-GGA-72187; mRNA 3'-end processing.
DR Reactome; R-GGA-72203; Processing of Capped Intron-Containing Pre-mRNA.
DR Reactome; R-GGA-73856; RNA Polymerase II Transcription Termination.
DR Reactome; R-GGA-77588; SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs.
DR Reactome; R-GGA-77595; Processing of Intronless Pre-mRNAs.
DR Reactome; R-GGA-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-GGA-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR PRO; PR:Q5ZJZ6; -.
DR Proteomes; UP000000539; Chromosome Z.
DR Bgee; ENSGALG00000002087; Expressed in spermatid and 14 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005845; C:mRNA cap binding complex; ISS:UniProtKB.
DR GO; GO:0005846; C:nuclear cap binding complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0000339; F:RNA cap binding; IBA:GO_Central.
DR GO; GO:0006370; P:7-methylguanosine mRNA capping; ISS:UniProtKB.
DR GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR GO; GO:0006406; P:mRNA export from nucleus; ISS:UniProtKB.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; ISS:UniProtKB.
DR GO; GO:0050684; P:regulation of mRNA processing; IBA:GO_Central.
DR GO; GO:0006446; P:regulation of translational initiation; ISS:UniProtKB.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR027159; CBP80.
DR InterPro; IPR015172; MIF4G-like_typ-1.
DR InterPro; IPR015174; MIF4G-like_typ-2.
DR InterPro; IPR003890; MIF4G-like_typ-3.
DR PANTHER; PTHR12412; PTHR12412; 1.
DR Pfam; PF02854; MIF4G; 1.
DR Pfam; PF09088; MIF4G_like; 1.
DR Pfam; PF09090; MIF4G_like_2; 1.
DR SMART; SM00543; MIF4G; 1.
DR SUPFAM; SSF48371; SSF48371; 3.
PE 2: Evidence at transcript level;
KW Coiled coil; Cytoplasm; mRNA capping; mRNA processing; mRNA splicing;
KW mRNA transport; Nonsense-mediated mRNA decay; Nucleus; Reference proteome;
KW RNA-mediated gene silencing; Translation regulation; Transport.
FT CHAIN 1..793
FT /note="Nuclear cap-binding protein subunit 1"
FT /id="PRO_0000239781"
FT DOMAIN 30..242
FT /note="MIF4G"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 668..692
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 686..716
FT /evidence="ECO:0000255"
SQ SEQUENCE 793 AA; 92564 MW; 174CB5601E92C0B3 CRC64;
MSRRRHSDDS DDSDGQPHKR RRTSEPSEIE ERLESLICRV GEKSNSSLES NLEGLAGVLE
ADLPNYKSKI LRILCTVARL LPEKLTVYTT LVGLLNARNY NFGGEFVEAM IRQLKECLKV
NMYNEAVHLV RFLSDLVNCH VIAAPSMVAM FENFVSVTQE EDVPQVRCDW YIFAFLSSLP
WVGKELYEKK DAEMDRLLSQ TESYLKRRQK IHVPMLQVWT ADKPHPQEEY LDCLWSQIQK
LKKDRWQERH ILRPYLAFDS ILCEALQHNL PPFTPPPHTE DSVYPMPRVI FRMFDYTDDP
EGPVMPGSHS VERFVIEENL HCIIKSHWKE RKTCAAQLLS YPGNNKIPLN YHIVEVIFAE
LFQLPSPPHI EVMYTTLLIE LCKLQPGSLP QVLAQATEML YMRLDTMNTT CVDRFINWFS
HHLSNFQFRW SWEDWSDCLT QDLEKPKPKF VREVLEKCMR LSYHQRIIDI VPASFSVLSP
ANPVCIYKYG DESNRSLPGY TVALCLTIAI KNKASNDEIF SILKDVPNPN QDDDDDEGFT
FNPLKIEVFV QTLLHLAAKS FSHSFSALAK FHEVFKTLAE SDEGKLHVLR VVYEVWKNHP
QMIAVLVDKM IRTQIVDCAA VANWIFSSEL AHDFTRFYIW EILHSTIRKM NKHVLKIHKE
LEDTKARLAR QHKRRDSDDD DRSSDREDGP LEEQIERLQE KVESAQSEQK NLFLVIFQRF
IMLLTEHLVR CETGGIDVFT PWYKSCIERL QQIFLQHHQI IQQYMVTFEN LLFTAELDHH
ILAVFQQFCA LQA
