NCBP1_DROPS
ID NCBP1_DROPS Reviewed; 800 AA.
AC Q29G82;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Nuclear cap-binding protein subunit 1;
DE AltName: Full=80 kDa nuclear cap-binding protein;
DE Short=CBP80;
DE Short=NCBP 80 kDa subunit;
GN Name=Cbp80; ORFNames=GA20048;
OS Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=46245;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MV2-25 / Tucson 14011-0121.94;
RX PubMed=15632085; DOI=10.1101/gr.3059305;
RA Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA Weinstock G.M., Gibbs R.A.;
RT "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT gene, and cis-element evolution.";
RL Genome Res. 15:1-18(2005).
CC -!- FUNCTION: Component of the cap-binding complex (CBC), which binds
CC cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in
CC various processes such as pre-mRNA splicing and RNA-mediated gene
CC silencing (RNAi). The CBC complex is involved in miRNA-mediated RNA
CC interference via its interaction with Ars2 and is required for primary
CC microRNAs (miRNAs) processing. Also involved in innate immunity via the
CC short interfering RNAs (siRNAs) processing machinery by restricting the
CC viral RNA production. In the CBC complex, Cbp80 does not bind directly
CC capped RNAs (m7GpppG-capped RNA) but is required to stabilize the
CC movement of the N-terminal loop of Cbp20 and lock the CBC into a high
CC affinity cap-binding state with the cap structure (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the nuclear cap-binding complex (CBC), a
CC heterodimer composed of Cbp80 and Cbp20 that interacts with m7GpppG-
CC capped RNA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NCBP1 family. {ECO:0000305}.
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DR EMBL; CH379064; EAL32228.1; -; Genomic_DNA.
DR RefSeq; XP_001355171.1; XM_001355135.3.
DR AlphaFoldDB; Q29G82; -.
DR SMR; Q29G82; -.
DR STRING; 7237.FBpp0283861; -.
DR EnsemblMetazoa; FBtr0285423; FBpp0283861; FBgn0080044.
DR GeneID; 4814640; -.
DR KEGG; dpo:Dpse_GA20048; -.
DR eggNOG; KOG1104; Eukaryota.
DR HOGENOM; CLU_013207_0_0_1; -.
DR InParanoid; Q29G82; -.
DR OMA; QPFKIPF; -.
DR PhylomeDB; Q29G82; -.
DR ChiTaRS; Cbp80; fly.
DR Proteomes; UP000001819; Chromosome X.
DR Bgee; FBgn0080044; Expressed in female reproductive system and 3 other tissues.
DR GO; GO:0005846; C:nuclear cap binding complex; IEA:InterPro.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000339; F:RNA cap binding; IEA:InterPro.
DR GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-KW.
DR GO; GO:0006406; P:mRNA export from nucleus; IEA:InterPro.
DR GO; GO:0045071; P:negative regulation of viral genome replication; IEA:EnsemblMetazoa.
DR GO; GO:0035194; P:post-transcriptional gene silencing by RNA; IEA:EnsemblMetazoa.
DR GO; GO:0031053; P:primary miRNA processing; IEA:EnsemblMetazoa.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR GO; GO:0030422; P:siRNA processing; IEA:EnsemblMetazoa.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR027159; CBP80.
DR InterPro; IPR015172; MIF4G-like_typ-1.
DR InterPro; IPR015174; MIF4G-like_typ-2.
DR InterPro; IPR003890; MIF4G-like_typ-3.
DR PANTHER; PTHR12412; PTHR12412; 1.
DR Pfam; PF02854; MIF4G; 1.
DR Pfam; PF09088; MIF4G_like; 1.
DR Pfam; PF09090; MIF4G_like_2; 1.
DR SMART; SM00543; MIF4G; 1.
DR SUPFAM; SSF48371; SSF48371; 3.
PE 3: Inferred from homology;
KW mRNA capping; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; RNA-mediated gene silencing.
FT CHAIN 1..800
FT /note="Nuclear cap-binding protein subunit 1"
FT /id="PRO_0000385238"
FT DOMAIN 31..243
FT /note="MIF4G"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 669..704
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 9
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 800 AA; 93069 MW; 48BC9F19C4DD50DA CRC64;
MSRRRAHDTE DEGYDHRRNK RRRVSENQEI EDRLESLILR VGERSTSSVE SNLEGLVSVL
EADLGTFRLK ILRILSDCAV RMPEKCTVYT TLVGLLNAKN YKFGGEFVDH MVKTFKESLK
MCRWDAARYS LRFLADLVNC HVISATSLLQ LLDTMIDVSN EDTVPQVRRD WFVFAVLSTL
PWVGRDLYEK KESALESLLL RIEVYLNKRS KKHHNALRVW SSDAPHPQEE YLDCLWAQIR
KLRQDNWAEK HIPRPYLTFD TILCEALQHN LPQIIPPPHN DAFVYPMPWV VYRMFDYTDC
PDGPNLPGAH SIERFLIEEH LHHIIETYHH ERKDCAAQLL SFPFKHKIPL EYCIVEVIFA
ELFHMPTPRY LDICYGSILI ELCKLQPATL PQVLAQATEI LFMRIDSMNT SCFDRFVNWF
SYHLSNFKFT WSWDEWDSCL LLDGEHPRPK FIQEVLQKCL RLSYHQRITE MMPTTYGKLI
PQVPVPNFKY ASEEAASLPG TAVAHQLVVA IRQKCSPEEV VNILKEIPNS GYSGEEMSDG
TFNALKIDVF VQTLLNLGSK SFSHSFAAIS KFHSVFRALA ETEEAQICVL HNIYELWSSH
QQMMVVLVDK LLKLQIVDCS AVATWIFSKE MTSEFTKMYL WEILHLTIKK MNKHVIKLNT
ELSVAKDKLS KADSSSSESD EDAPTKRKKP ITHADKPSEE AVERMEEKLE AANVNQKRLF
LIVFQRFIMI LSEHMLRSDT DGRDPDTDWY RWTIGRLQQV FLMHHEQVQK YSSTLETLLF
TSDLDTHILE VFQQFVALRA
