ID   NCBP2_BOMMO             Reviewed;         154 AA.
AC   Q1HE01;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2006, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Nuclear cap-binding protein subunit 2;
DE   AltName: Full=20 kDa nuclear cap-binding protein;
DE   AltName: Full=NCBP 20 kDa subunit;
DE            Short=CBP20;
OS   Bombyx mori (Silk moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC   Bombycidae; Bombycinae; Bombyx.
OX   NCBI_TaxID=7091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Wang L.-L., Chen K.-P., Yao Q., Hu Z.-G., Gao G.-T.;
RT   "RNA binding proteins in Bombyx mori.";
RL   Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the cap-binding complex (CBC), which binds co-
CC       transcriptionally to the 5' cap of pre-mRNAs and is involved in various
CC       processes such as pre-mRNA splicing and RNA-mediated gene silencing
CC       (RNAi). The CBC complex is involved in miRNA-mediated RNA interference
CC       and is required for primary microRNAs (miRNAs) processing. Also
CC       involved in innate immunity via the short interfering RNAs (siRNAs)
CC       processing machinery by restricting the viral RNA production. In the
CC       CBC complex, Cbp20 recognizes and binds capped RNAs (m7GpppG-capped
CC       RNA) but requires Cbp80 to stabilize the movement of its N-terminal
CC       loop and lock the CBC into a high affinity cap-binding state with the
CC       cap structure (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the nuclear cap-binding complex (CBC), a
CC       heterodimer composed of Cbp80 and Cbp20 that interacts with m7GpppG-
CC       capped RNA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RRM NCBP2 family. {ECO:0000305}.
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DR   EMBL; DQ497196; ABF55961.1; -; mRNA.
DR   RefSeq; NP_001037598.1; NM_001044133.1.
DR   AlphaFoldDB; Q1HE01; -.
DR   SMR; Q1HE01; -.
DR   STRING; 7091.BGIBMGA007585-TA; -.
DR   GeneID; 733050; -.
DR   KEGG; bmor:733050; -.
DR   CTD; 42166; -.
DR   eggNOG; KOG0121; Eukaryota.
DR   HOGENOM; CLU_070952_1_1_1; -.
DR   OrthoDB; 1421503at2759; -.
DR   Proteomes; UP000005204; Unassembled WGS sequence.
DR   GO; GO:0005846; C:nuclear cap binding complex; IEA:InterPro.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000339; F:RNA cap binding; IEA:InterPro.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR   GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IEA:InterPro.
DR   CDD; cd12240; RRM_NCBP2; 1.
DR   Gene3D; 3.30.70.330; -; 1.
DR   InterPro; IPR027157; NCBP2.
DR   InterPro; IPR034148; NCBP2_RRM.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   PANTHER; PTHR18847; PTHR18847; 1.
DR   Pfam; PF00076; RRM_1; 1.
DR   SMART; SM00360; RRM; 1.
DR   SUPFAM; SSF54928; SSF54928; 1.
DR   PROSITE; PS50102; RRM; 1.
PE   2: Evidence at transcript level;
KW   mRNA processing; mRNA splicing; Nucleus; Reference proteome; RNA-binding;
KW   RNA-mediated gene silencing.
FT   CHAIN           1..154
FT                   /note="Nuclear cap-binding protein subunit 2"
FT                   /id="PRO_0000385260"
FT   DOMAIN          30..108
FT                   /note="RRM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   BINDING         10
FT                   /ligand="mRNA"
FT                   /ligand_id="ChEBI:CHEBI:33699"
FT                   /ligand_part="mRNA cap"
FT                   /evidence="ECO:0000250"
FT   BINDING         33
FT                   /ligand="mRNA"
FT                   /ligand_id="ChEBI:CHEBI:33699"
FT                   /ligand_part="mRNA cap"
FT                   /evidence="ECO:0000250"
FT   BINDING         102..106
FT                   /ligand="mRNA"
FT                   /ligand_id="ChEBI:CHEBI:33699"
FT                   /ligand_part="mRNA cap"
FT                   /evidence="ECO:0000250"
FT   BINDING         113..117
FT                   /ligand="mRNA"
FT                   /ligand_id="ChEBI:CHEBI:33699"
FT                   /ligand_part="mRNA cap"
FT                   /evidence="ECO:0000250"
FT   BINDING         123..124
FT                   /ligand="mRNA"
FT                   /ligand_id="ChEBI:CHEBI:33699"
FT                   /ligand_part="mRNA cap"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   154 AA;  17920 MW;  79A1022AC0BCB7AF CRC64;
     MNSSIEISSY RDQHFKGSRS EQEKLLKASS TLYMGNLSFY TTEEQIYELY SRCGDIRRVI
     MGLDKYKKTP CGFCFVEYYA REDAENCMRY INGTRLDDRI IRCDWDAGFI EGRQYGRGKT
     GGQVRDEYRT DYDGGRGGYG KIIAQKINPN TLER