NCBP2_CAEEL
ID NCBP2_CAEEL Reviewed; 158 AA.
AC Q93594; G3MU41; G3MU42; G3MU43;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Nuclear cap-binding protein subunit 2;
DE AltName: Full=20 kDa nuclear cap-binding protein;
DE AltName: Full=NCBP 20 kDa subunit;
DE Short=CBP20;
GN Name=ncbp-2; Synonyms=cbp-20; ORFNames=F26A3.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP DISRUPTION PHENOTYPE.
RX PubMed=16207815; DOI=10.1091/mbc.e05-07-0622;
RA Lall S., Piano F., Davis R.E.;
RT "Caenorhabditis elegans decapping proteins: localization and functional
RT analysis of Dcp1, Dcp2, and DcpS during embryogenesis.";
RL Mol. Biol. Cell 16:5880-5890(2005).
CC -!- FUNCTION: Component of the cap-binding complex (CBC), which binds co-
CC transcriptionally to the 5' cap of pre-mRNAs and is involved in various
CC processes such as pre-mRNA splicing and RNA-mediated gene silencing
CC (RNAi). The CBC complex is involved in miRNA-mediated RNA interference
CC and is required for primary microRNAs (miRNAs) processing. In the CBC
CC complex, ncbp-2 recognizes and binds capped RNAs (m7GpppG-capped RNA)
CC but requires ncbp-1 to stabilize the movement of its N-terminal loop
CC and lock the CBC into a high affinity cap-binding state with the cap
CC structure (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the nuclear cap-binding complex (CBC), a
CC heterodimer composed of ncbp-1 and ncbp-2 that interacts with m7GpppG-
CC capped RNA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=a;
CC IsoId=Q93594-1; Sequence=Displayed;
CC Name=b;
CC IsoId=Q93594-2; Sequence=VSP_044210;
CC Name=c;
CC IsoId=Q93594-3; Sequence=VSP_044211;
CC -!- DISRUPTION PHENOTYPE: Lethality in 98.7% of embryos.
CC {ECO:0000269|PubMed:16207815}.
CC -!- SIMILARITY: Belongs to the RRM NCBP2 family. {ECO:0000305}.
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DR EMBL; Z78419; CCD31067.1; -; Genomic_DNA.
DR EMBL; Z78419; CCD31068.1; -; Genomic_DNA.
DR EMBL; Z78419; CCD31069.1; -; Genomic_DNA.
DR PIR; T21382; T21382.
DR RefSeq; NP_001250552.1; NM_001263623.1.
DR RefSeq; NP_001250553.1; NM_001263624.1. [Q93594-2]
DR RefSeq; NP_001250554.1; NM_001263625.1. [Q93594-3]
DR AlphaFoldDB; Q93594; -.
DR SMR; Q93594; -.
DR BioGRID; 49734; 3.
DR ComplexPortal; CPX-957; Nuclear cap-binding complex.
DR STRING; 6239.F26A3.2a; -.
DR EPD; Q93594; -.
DR PaxDb; Q93594; -.
DR PeptideAtlas; Q93594; -.
DR EnsemblMetazoa; F26A3.2a.1; F26A3.2a.1; WBGene00009141. [Q93594-1]
DR EnsemblMetazoa; F26A3.2a.2; F26A3.2a.2; WBGene00009141. [Q93594-1]
DR EnsemblMetazoa; F26A3.2b.1; F26A3.2b.1; WBGene00009141. [Q93594-2]
DR EnsemblMetazoa; F26A3.2c.1; F26A3.2c.1; WBGene00009141. [Q93594-3]
DR GeneID; 184953; -.
DR UCSC; F26A3.2; c. elegans. [Q93594-1]
DR CTD; 184953; -.
DR WormBase; F26A3.2a; CE46329; WBGene00009141; ncbp-2. [Q93594-1]
DR WormBase; F26A3.2b; CE46444; WBGene00009141; ncbp-2. [Q93594-2]
DR WormBase; F26A3.2c; CE46506; WBGene00009141; ncbp-2. [Q93594-3]
DR eggNOG; KOG0121; Eukaryota.
DR GeneTree; ENSGT00390000003197; -.
DR HOGENOM; CLU_070952_2_1_1; -.
DR InParanoid; Q93594; -.
DR OMA; AENCMRY; -.
DR OrthoDB; 1421503at2759; -.
DR PhylomeDB; Q93594; -.
DR Reactome; R-CEL-111367; SLBP independent Processing of Histone Pre-mRNAs.
DR Reactome; R-CEL-113418; Formation of the Early Elongation Complex.
DR Reactome; R-CEL-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-CEL-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-CEL-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR Reactome; R-CEL-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-CEL-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-CEL-6803529; FGFR2 alternative splicing.
DR Reactome; R-CEL-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-CEL-72086; mRNA Capping.
DR Reactome; R-CEL-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-CEL-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-CEL-72187; mRNA 3'-end processing.
DR Reactome; R-CEL-73856; RNA Polymerase II Transcription Termination.
DR Reactome; R-CEL-77588; SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs.
DR Reactome; R-CEL-77595; Processing of Intronless Pre-mRNAs.
DR Reactome; R-CEL-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-CEL-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR PRO; PR:Q93594; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00009141; Expressed in embryo and 4 other tissues.
DR GO; GO:0005845; C:mRNA cap binding complex; IC:ComplexPortal.
DR GO; GO:0005846; C:nuclear cap binding complex; IBA:GO_Central.
DR GO; GO:0005635; C:nuclear envelope; IDA:WormBase.
DR GO; GO:0034399; C:nuclear periphery; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0000339; F:RNA cap binding; IBA:GO_Central.
DR GO; GO:0035195; P:miRNA-mediated gene silencing; IC:ComplexPortal.
DR GO; GO:0031124; P:mRNA 3'-end processing; IC:ComplexPortal.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IEA:InterPro.
DR GO; GO:0006406; P:mRNA export from nucleus; IC:ComplexPortal.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0042789; P:mRNA transcription by RNA polymerase II; IC:ComplexPortal.
DR GO; GO:0000956; P:nuclear-transcribed mRNA catabolic process; IC:ComplexPortal.
DR GO; GO:0031053; P:primary miRNA processing; IDA:ComplexPortal.
DR CDD; cd12240; RRM_NCBP2; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR027157; NCBP2.
DR InterPro; IPR034148; NCBP2_RRM.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR PANTHER; PTHR18847; PTHR18847; 1.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 3: Inferred from homology;
KW Alternative splicing; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; RNA-binding; RNA-mediated gene silencing.
FT CHAIN 1..158
FT /note="Nuclear cap-binding protein subunit 2"
FT /id="PRO_0000385257"
FT DOMAIN 37..115
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 123..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 125..144
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 17
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="mRNA cap"
FT /evidence="ECO:0000250"
FT BINDING 40
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="mRNA cap"
FT /evidence="ECO:0000250"
FT BINDING 109..113
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="mRNA cap"
FT /evidence="ECO:0000250"
FT BINDING 120..124
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="mRNA cap"
FT /evidence="ECO:0000250"
FT BINDING 130..131
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="mRNA cap"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..102
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_044211"
FT VAR_SEQ 1..67
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_044210"
SQ SEQUENCE 158 AA; 18438 MW; 18AB5D6FF8131E50 CRC64;
MVFDPRTLDN PKEISAYRDQ RYQGTVRDQE TALRTSCTLY VGNLSYYTKE DQVYELFGRA
GDVRRVIMGL DRFKKTPCGF CFVEYYTRED AELALQNISN TRMDDRVIRA DWDAGFIEGR
QYGRGKHGGQ VRDEYRKDYD PERGGYNRAI AQKGGDRQ
