ID   NCBP2_IXOSC             Reviewed;         152 AA.
AC   B7P877;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Nuclear cap-binding protein subunit 2;
DE   AltName: Full=20 kDa nuclear cap-binding protein;
DE   AltName: Full=NCBP 20 kDa subunit;
DE            Short=CBP20;
GN   Name=Cbp20; ORFNames=ISCW017479;
OS   Ixodes scapularis (Black-legged tick) (Deer tick).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Ixodinae; Ixodes.
OX   NCBI_TaxID=6945;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Wikel;
RG   Ixodes scapularis Genome Project Consortium;
RA   Caler E., Hannick L.I., Bidwell S., Joardar V., Thiagarajan M., Amedeo P.,
RA   Galinsky K.J., Schobel S., Inman J., Hostetler J., Miller J., Hammond M.,
RA   Megy K., Lawson D., Kodira C., Sutton G., Meyer J., Hill C.A., Birren B.,
RA   Nene V., Collins F., Alarcon-Chaidez F., Wikel S., Strausberg R.;
RT   "Annotation of Ixodes scapularis.";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the cap-binding complex (CBC), which binds co-
CC       transcriptionally to the 5' cap of pre-mRNAs and is involved in various
CC       processes such as pre-mRNA splicing and RNA-mediated gene silencing
CC       (RNAi). The CBC complex is involved in miRNA-mediated RNA interference
CC       and is required for primary microRNAs (miRNAs) processing. Also
CC       involved in innate immunity via the short interfering RNAs (siRNAs)
CC       processing machinery by restricting the viral RNA production. In the
CC       CBC complex, Cbp20 recognizes and binds capped RNAs (m7GpppG-capped
CC       RNA) but requires Cbp80 to stabilize the movement of its N-terminal
CC       loop and lock the CBC into a high affinity cap-binding state with the
CC       cap structure (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the nuclear cap-binding complex (CBC), a
CC       heterodimer composed of Cbp80 and Cbp20 that interacts with m7GpppG-
CC       capped RNA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RRM NCBP2 family. {ECO:0000305}.
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DR   EMBL; DS656359; EEC02799.1; -; Genomic_DNA.
DR   RefSeq; XP_002401570.1; XM_002401526.1.
DR   AlphaFoldDB; B7P877; -.
DR   SMR; B7P877; -.
DR   STRING; 6945.B7P877; -.
DR   EnsemblMetazoa; ISCI017479-RA; ISCI017479-PA; ISCI017479.
DR   EnsemblMetazoa; XM_002401526.5; XP_002401570.1; LOC8026041.
DR   GeneID; 8026041; -.
DR   KEGG; isc:IscW_ISCW017479; -.
DR   VEuPathDB; VectorBase:ISCI017479; -.
DR   VEuPathDB; VectorBase:ISCW017479; -.
DR   HOGENOM; CLU_070952_2_0_1; -.
DR   InParanoid; B7P877; -.
DR   OMA; AENCMRY; -.
DR   OrthoDB; 1421503at2759; -.
DR   PhylomeDB; B7P877; -.
DR   Proteomes; UP000001555; Unassembled WGS sequence.
DR   GO; GO:0005846; C:nuclear cap binding complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000339; F:RNA cap binding; IBA:GO_Central.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR   GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IEA:InterPro.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   CDD; cd12240; RRM_NCBP2; 1.
DR   Gene3D; 3.30.70.330; -; 1.
DR   InterPro; IPR027157; NCBP2.
DR   InterPro; IPR034148; NCBP2_RRM.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   PANTHER; PTHR18847; PTHR18847; 1.
DR   Pfam; PF00076; RRM_1; 1.
DR   SMART; SM00360; RRM; 1.
DR   SUPFAM; SSF54928; SSF54928; 1.
DR   PROSITE; PS50102; RRM; 1.
PE   3: Inferred from homology;
KW   mRNA processing; mRNA splicing; Nucleus; Reference proteome; RNA-binding;
KW   RNA-mediated gene silencing.
FT   CHAIN           1..152
FT                   /note="Nuclear cap-binding protein subunit 2"
FT                   /id="PRO_0000385262"
FT   DOMAIN          28..106
FT                   /note="RRM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   BINDING         8
FT                   /ligand="mRNA"
FT                   /ligand_id="ChEBI:CHEBI:33699"
FT                   /ligand_part="mRNA cap"
FT                   /evidence="ECO:0000250"
FT   BINDING         31
FT                   /ligand="mRNA"
FT                   /ligand_id="ChEBI:CHEBI:33699"
FT                   /ligand_part="mRNA cap"
FT                   /evidence="ECO:0000250"
FT   BINDING         100..104
FT                   /ligand="mRNA"
FT                   /ligand_id="ChEBI:CHEBI:33699"
FT                   /ligand_part="mRNA cap"
FT                   /evidence="ECO:0000250"
FT   BINDING         111..115
FT                   /ligand="mRNA"
FT                   /ligand_id="ChEBI:CHEBI:33699"
FT                   /ligand_part="mRNA cap"
FT                   /evidence="ECO:0000250"
FT   BINDING         121..122
FT                   /ligand="mRNA"
FT                   /ligand_id="ChEBI:CHEBI:33699"
FT                   /ligand_part="mRNA cap"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   152 AA;  17485 MW;  6C8CF3B36A5C5918 CRC64;
     MSVDLSSYRD QHFKGSRSEQ ERLLRLSTTL YVGNMSFYTT EEQIYELFSK CGDVKKVIMG
     LDRFHKTPCG FCFVEYYTRE DAENAIRYVN GTKLDDRIIR TDWDAGFVEG RQFGRGKSGG
     QVRDEYRTDY DSGRGGYGKL MAQRVVPPPA VV