ID   NCCA_ALCXX              Reviewed;        1076 AA.
AC   Q44586;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Nickel-cobalt-cadmium resistance protein NccA;
GN   Name=nccA;
OS   Alcaligenes xylosoxydans xylosoxydans (Achromobacter xylosoxidans).
OG   Plasmid pTOM9.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Achromobacter.
OX   NCBI_TaxID=85698;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=31A;
RX   PubMed=7961470; DOI=10.1128/jb.176.22.7045-7054.1994;
RA   Schmidt T., Schlegel H.G.;
RT   "Combined nickel-cobalt-cadmium resistance encoded by the ncc locus of
RT   Alcaligenes xylosoxidans 31A.";
RL   J. Bacteriol. 176:7045-7054(1994).
CC   -!- FUNCTION: Component of the NCC cation-efflux system that confers
CC       resistance to nickel, cobalt and cadmium. May form a membrane tunnel,
CC       which allows ion transport across the membrane.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC       (TC 2.A.6) family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L31363; AAA65106.1; -; Genomic_DNA.
DR   PIR; I39580; I39580.
DR   AlphaFoldDB; Q44586; -.
DR   SMR; Q44586; -.
DR   TCDB; 2.A.6.1.12; the resistance-nodulation-cell division (rnd) superfamily.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008324; F:cation transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046686; P:response to cadmium ion; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.2090.10; -; 2.
DR   InterPro; IPR027463; AcrB_DN_DC_subdom.
DR   InterPro; IPR001036; Acrflvin-R.
DR   InterPro; IPR004763; CzcA/CusA/SilA/NccA/HelA/CnrA.
DR   PANTHER; PTHR32063; PTHR32063; 1.
DR   Pfam; PF00873; ACR_tran; 1.
DR   PRINTS; PR00702; ACRIFLAVINRP.
DR   SUPFAM; SSF82714; SSF82714; 2.
DR   TIGRFAMs; TIGR00914; 2A0601; 1.
PE   3: Inferred from homology;
KW   Cadmium; Cadmium resistance; Cell membrane; Cobalt; Membrane; Nickel;
KW   Plasmid; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1076
FT                   /note="Nickel-cobalt-cadmium resistance protein NccA"
FT                   /id="PRO_0000161845"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        367..387
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        391..411
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        419..439
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        476..496
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        503..523
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        562..582
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        904..924
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        929..949
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        960..980
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1004..1024
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1036..1056
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1076 AA;  115751 MW;  AB58E0333181DD55 CRC64;
     MIESILSGAV RFRWLVLFLT AVVGAIGAWQ LNLLPIDVTP DITNKQVQIN TVVPTLSPVE
     VEKRVTYPIE TAIAGLNGVE NMRSLSRNGF SQVTVIFKES SNLYFMRQQV TERLAQARPN
     LPAGVEPQMG PVSTGLGEVF HYSVEYEFPD GKGAKVKDGE PGWQSDGSFL TERGERLTDR
     VSKLAYLRTV QDWIIRPQLR TTAGVADVDS LGGYVKQFVV EPDAAKMAAY GISFEELAQA
     LEDANLSVGA NFIRRSGESY LVRADARIKS ADEIARAVIA QRQGVPITVG QVANINVGGE
     LRSGAASRNG YETVVGSALM LVGANSRTVA QAVGDKLEEI KKTLPPGVVI VPTLNRSQLV
     MATIKTVAKN LVEGAALVVV ILFALLGNWR AAVIAALVIP LSLLISAIGM NGLNISGNLM
     SLGALDFGLI IDGAVIIVEN SLRRLAERQH HEGRLLTLKE RLEEVILSSR EMVRPTVYGQ
     LVIFMVFLPC LTFQGVEGKM FSPMVITLML ALASAFVLSL TFVPAMVAVL LRKKVSEKEV
     RVIAVTKERY RPLLERAVAR PMPFLGAALV TLALAAMAFT FVGREFMPTL DEQNLNLSSV
     RIPSTSIDQS VAIDLPLERA VLSLPEVQTV YSKAGTASLA ADPMPPNASD NYIILKPKSE
     WPEGITTKEQ VIERIREKTA PMVSNNYDVT QPIQMRFNEL IGGVRSDVAV KIYGENLDDL
     ASTAQKIAAV LRKTPGATDT RVPLTGGFPT FDIVFDRAAI ARYGLTVKEV ADTVAAAMAG
     RPSGQIFDGD RRYDIVIRLP GQQRENLDVL GALPVMLPAV EGQPRASVPL RQLVQFRFTQ
     GLNEVSRDNG KRRVYVEANV GGRDLGSFVD DAAKRIAAEV KLPPGMYIEW GGQFQNLQAA
     TQRLAIIVPL CFILIAATLY MAIGSAALTA TVLTAVPLAL AGGVFALVLR DIPFSISASV
     GFIAVSGVAV LNGLVLISAI RKRLEDGAAP NEAVIEGAME RVRPVLMTAL VASLGFVPMA
     IATGTGAEVQ KPLATVVIGG LITATVLTLF VLPAVCGMVL RRQKKLEKPG GELLEA