ID   NCCH_ALCXX              Reviewed;         186 AA.
AC   Q44583;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=RNA polymerase sigma factor NccH;
GN   Name=nccH;
OS   Alcaligenes xylosoxydans xylosoxydans (Achromobacter xylosoxidans).
OG   Plasmid pTOM9.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Achromobacter.
OX   NCBI_TaxID=85698;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=31A;
RX   PubMed=7961470; DOI=10.1128/jb.176.22.7045-7054.1994;
RA   Schmidt T., Schlegel H.G.;
RT   "Combined nickel-cobalt-cadmium resistance encoded by the ncc locus of
RT   Alcaligenes xylosoxidans 31A.";
RL   J. Bacteriol. 176:7045-7054(1994).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor regulates the genes for a membrane-located
CC       efflux system that confers resistance to nickel, cobalt and cadmium.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L31363; AAA65103.1; -; Genomic_DNA.
DR   PIR; I39577; I39577.
DR   AlphaFoldDB; Q44583; -.
DR   SMR; Q44583; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0046686; P:response to cadmium ion; IEA:UniProtKB-KW.
DR   GO; GO:0006950; P:response to stress; IEA:UniProt.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR039425; RNA_pol_sigma-70-like.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000838; RNA_pol_sigma70_ECF_CS.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR43133; PTHR43133; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF08281; Sigma70_r4_2; 1.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS01063; SIGMA70_ECF; 1.
PE   3: Inferred from homology;
KW   Cadmium; Cadmium resistance; Cobalt; DNA-binding; Nickel; Plasmid;
KW   Sigma factor; Transcription; Transcription regulation.
FT   CHAIN           1..186
FT                   /note="RNA polymerase sigma factor NccH"
FT                   /id="PRO_0000094010"
FT   DNA_BIND        152..171
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           49..62
FT                   /note="Polymerase core binding"
SQ   SEQUENCE   186 AA;  20749 MW;  70D4CD5CC3E3C1FB CRC64;
     MTDPSVDEAL ARRAAQGDQY AFGQLVQRHG RALAQAARSF GVPESDVDDI VQDTFIAAWH
     ALDDFDSDKL FRPWLFRIGL NKMRDLRRFR QVRHFLFGAK DIEDAELTSD VPGPEREVSA
     RLELAKIMQV LNRLDLASRE IIILTSFVGM SHPEAAMALG TSAKAVESRV ARARAKLTAL
     LQSSQV