NCED1_SOLLC
ID NCED1_SOLLC Reviewed; 605 AA.
AC O24023;
DT 07-NOV-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 2.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic {ECO:0000303|Ref.2};
DE Short=LeNCED1 {ECO:0000303|Ref.2};
DE Short=SlNCED1 {ECO:0000303|PubMed:22345638};
DE EC=1.13.11.51 {ECO:0000250|UniProtKB:Q9LRR7};
DE AltName: Full=Nine-cis-epoxycarotenoid dioxygenase 1 {ECO:0000303|Ref.2};
DE Flags: Precursor;
GN Name=NCED1 {ECO:0000303|Ref.2};
GN Synonyms=NCE {ECO:0000303|Ref.1}, NOTABILIS {ECO:0000303|Ref.2};
GN OrderedLocusNames=Solyc07g056570.1.1 {ECO:0000305};
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION BY DROUGHT.
RX DOI=10.1093/jxb/48.12.2111;
RA Burbidge A., Grieve T.M., Jackson A., Thompson A., Taylor I.B.;
RT "Structure and expression of a cDNA encoding a putative neoxanthin cleavage
RT enzyme (NCE) isolated from a wilt-related tomato (Lycopersicon esculentum
RT Mill.) library.";
RL J. Exp. Bot. 48:2111-2112(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, PATHWAY,
RP AND INDUCTION BY DROUGHT.
RC STRAIN=cv. Moneymaker;
RX DOI=10.1111/j.1365-3040.2003.01164.x;
RA Thompson A.J., Thorne E.T., Burbridge A., Jackson A.C., Sharp R.E.,
RA Taylor I.B.;
RT "Complementation of notabilis, an abscisic acid-deficient mutant of tomato:
RT importance of sequence context and utility of partial complementation.";
RL Plant Cell Environ. 27:459-471(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Heinz 1706;
RX PubMed=22660326; DOI=10.1038/nature11119;
RG Tomato Genome Consortium;
RT "The tomato genome sequence provides insights into fleshy fruit
RT evolution.";
RL Nature 485:635-641(2012).
RN [4]
RP FUNCTION, DEVELOPMENTAL STAGE, AND INDUCTION BY DEHYDRATION.
RC STRAIN=cv. Ailsa Craig; TISSUE=Fruit;
RX PubMed=19246595; DOI=10.1093/jxb/erp026;
RA Zhang M., Yuan B., Leng P.;
RT "The role of ABA in triggering ethylene biosynthesis and ripening of tomato
RT fruit.";
RL J. Exp. Bot. 60:1579-1588(2009).
RN [5]
RP DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, REPRESSION BY POLLINATION; AUXIN
RP AND GIBBERELLIC ACID, AND INDUCTION BY ABSCISIC ACID.
RC STRAIN=cv. Moneymaker;
RX PubMed=19322584; DOI=10.1007/s00425-009-0913-7;
RA Nitsch L.M., Oplaat C., Feron R., Ma Q., Wolters-Arts M., Hedden P.,
RA Mariani C., Vriezen W.H.;
RT "Abscisic acid levels in tomato ovaries are regulated by LeNCED1 and
RT SlCYP707A1.";
RL Planta 229:1335-1346(2009).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Jia Bao;
RX PubMed=22345638; DOI=10.1093/jxb/ers026;
RA Sun L., Yuan B., Zhang M., Wang L., Cui M., Wang Q., Leng P.;
RT "Fruit-specific RNAi-mediated suppression of SlNCED1 increases both
RT lycopene and beta-carotene contents in tomato fruit.";
RL J. Exp. Bot. 63:3097-3108(2012).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, BIOTECHNOLOGY, DEVELOPMENTAL STAGE, AND
RP TISSUE SPECIFICITY.
RC STRAIN=cv. Jia Bao;
RX PubMed=22108525; DOI=10.1104/pp.111.186866;
RA Sun L., Sun Y., Zhang M., Wang L., Ren J., Cui M., Wang Y., Ji K., Li P.,
RA Li Q., Chen P., Dai S., Duan C., Wu Y., Leng P.;
RT "Suppression of 9-cis-epoxycarotenoid dioxygenase, which encodes a key
RT enzyme in abscisic acid biosynthesis, alters fruit texture in transgenic
RT tomato.";
RL Plant Physiol. 158:283-298(2012).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION BY ABSCISIC
RP ACID AND DEHYDRATION, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Jia Bao;
RX PubMed=25039074; DOI=10.1093/jxb/eru288;
RA Ji K., Kai W., Zhao B., Sun Y., Yuan B., Dai S., Li Q., Chen P., Wang Y.,
RA Pei Y., Wang H., Guo Y., Leng P.;
RT "SlNCED1 and SlCYP707A2: key genes involved in ABA metabolism during tomato
RT fruit ripening.";
RL J. Exp. Bot. 65:5243-5255(2014).
RN [9]
RP INDUCTION BY SALICYLIC ACID.
RC STRAIN=cv. Rio Fuego;
RX PubMed=26086888; DOI=10.1016/j.jplph.2015.05.010;
RA Horvath E., Csiszar J., Galle A., Poor P., Szepesi A., Tari I.;
RT "Hardening with salicylic acid induces concentration-dependent changes in
RT abscisic acid biosynthesis of tomato under salt stress.";
RL J. Plant Physiol. 183:54-63(2015).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Jia Bao;
RX PubMed=29632966; DOI=10.1007/s00018-018-2809-9;
RA Dai S., Kai W., Liang B., Wang J., Jiang L., Du Y., Sun Y., Leng P.;
RT "The functional analysis of SlNCED1 in tomato pollen development.";
RL Cell. Mol. Life Sci. 75:3457-3472(2018).
RN [11]
RP INDUCTION BY NAP2.
RX PubMed=29760199; DOI=10.1104/pp.18.00292;
RA Ma X., Zhang Y., Tureckova V., Xue G.-P., Fernie A.R., Mueller-Roeber B.,
RA Balazadeh S.;
RT "The NAC transcription factor SlNAP2 regulates leaf senescence and fruit
RT yield in tomato.";
RL Plant Physiol. 177:1286-1302(2018).
CC -!- FUNCTION: Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity.
CC Catalyzes the first step of abscisic-acid (ABA) biosynthesis from
CC carotenoids. Required for ABA accumulation upon drought (Ref.2).
CC Required for ABA-mediated regulation of anther/pollen development,
CC including metabolism, cell wall modification and transcription level
CC (PubMed:29632966). Positive regulator of fruit ripening involved in the
CC biosynthesis of abscisic acid (ABA); initiates ABA biosynthesis at the
CC onset of fruit ripening (PubMed:19246595, PubMed:22108525,
CC PubMed:25039074). Modulates the degree of pigmentation and carotenoid
CC composition as well as pectin catabolism during ripening and may
CC regulate the ethylene production and action in climacteric tomato fruit
CC (PubMed:22345638, PubMed:22108525). {ECO:0000269|PubMed:19246595,
CC ECO:0000269|PubMed:22108525, ECO:0000269|PubMed:22345638,
CC ECO:0000269|PubMed:25039074, ECO:0000269|PubMed:29632966,
CC ECO:0000269|Ref.2}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a
CC 12'-apo-carotenal; Xref=Rhea:RHEA:23328, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:51972, ChEBI:CHEBI:51973;
CC EC=1.13.11.51; Evidence={ECO:0000250|UniProtKB:Q9LRR7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-
CC dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-xanthoxin;
CC Xref=Rhea:RHEA:16541, ChEBI:CHEBI:15379, ChEBI:CHEBI:32304,
CC ChEBI:CHEBI:34597, ChEBI:CHEBI:35305; EC=1.13.11.51;
CC Evidence={ECO:0000250|UniProtKB:Q9LRR7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-
CC didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-
CC xanthoxin; Xref=Rhea:RHEA:19677, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:34596, ChEBI:CHEBI:35306;
CC EC=1.13.11.51; Evidence={ECO:0000250|UniProtKB:Q9LRR7};
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000250|UniProtKB:O24592};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000250|UniProtKB:O24592};
CC -!- PATHWAY: Plant hormone biosynthesis; abscisate biosynthesis.
CC {ECO:0000269|Ref.2}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC {ECO:0000250|UniProtKB:Q9LRR7}. Note=Partially bound to the thylakoid.
CC {ECO:0000250|UniProtKB:Q9LRR7}.
CC -!- TISSUE SPECIFICITY: Expressed in developing and ripening fruits
CC (PubMed:22345638, PubMed:22108525). Highly expressed in pulp
CC (PubMed:25039074). Observed in unpollinated ovaries (e.g. ovules,
CC placenta and pericarp) (PubMed:19322584). Expressed in flowers
CC (PubMed:29632966). {ECO:0000269|PubMed:19322584,
CC ECO:0000269|PubMed:22108525, ECO:0000269|PubMed:22345638,
CC ECO:0000269|PubMed:25039074, ECO:0000269|PubMed:29632966}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the meristem during gametogenesis and
CC mainly in ovule, stigma, anther/pollen and vascular tissues during
CC flower development. In flower buds, present in petal primordium and
CC apical meristem. Later expressed in stamen primordium and pistil
CC meristem after petal formation. Accumulates in stamen, pistil and
CC vascular tissues of the bud base. Highly expressed in sporogenous cells
CC in anther, pistil stigma, ovules, pollen grains, anther walls, and
CC basal vascular bundle. In anthers, first present at low levels, then
CC increases rapidly to reach a peak at stages 13-14 (when microspores
CC transform into vacuolated pollen grains after mitotic divisions) before
CC declines gradually (PubMed:29632966). In fruits, levels decline from
CC the immature stage to the mature green stage and then rapidly increase
CC to a peak at the turning stage (PubMed:22345638, PubMed:22108525,
CC PubMed:25039074). Highly expressed in fruits at the onset of ripening
CC when the abscisic-acid (ABA) content becomes high (PubMed:19246595,
CC PubMed:22345638, PubMed:22108525). Progressive level reduction in
CC ovaries (e.g. ovules, placenta and pericarp) after pollination
CC (PubMed:19322584). {ECO:0000269|PubMed:19246595,
CC ECO:0000269|PubMed:19322584, ECO:0000269|PubMed:22108525,
CC ECO:0000269|PubMed:22345638, ECO:0000269|PubMed:25039074,
CC ECO:0000269|PubMed:29632966}.
CC -!- INDUCTION: Induced by drought (Ref.1, Ref.2, PubMed:25039074).
CC Accumulates in dehydration-treated fruits (PubMed:19246595). Repressed
CC after pollination. Inhibited by gibberellic acid (GA(3)) and auxin (4-
CC Cl-IAA) treatments (PubMed:19322584). Induced by abscisic acid (ABA)
CC (PubMed:19322584, PubMed:25039074). Up-regulated by salicylic acid (SA)
CC in roots; this accumulation is repressed by salt stress (NaCl) with
CC high SA treatment but with low SA (PubMed:25039074). Regulated by the
CC NAC transcription factor NAP2 (PubMed:29760199).
CC {ECO:0000269|PubMed:19246595, ECO:0000269|PubMed:19322584,
CC ECO:0000269|PubMed:25039074, ECO:0000269|PubMed:29760199,
CC ECO:0000269|Ref.1, ECO:0000269|Ref.2}.
CC -!- DISRUPTION PHENOTYPE: The mutant notabilis (not) is abscisic acid
CC (ABA)-deficient (including in response to dehydration) and exhibits a
CC reduced growth and lower ethylene levels (Ref.2). Abnormal development
CC of anther/pollen leading to serious pollen deterioration
CC (PubMed:29632966). Fruit-specific suppression leads to reduced ABA
CC accumulation resulting in an increase in ethylene levels, by increasing
CC the transcription of genes related to the synthesis of ethylene during
CC ripening. Deep red fruits which accumulate higher levels of lycopene
CC and beta-carotene probably due to the metabolism of 'backlogged' carbon
CC as a result of disturbed ABA signaling (PubMed:22345638). When
CC disrupted in fruits, altered expression of genes involved in ABA
CC metabolism and signaling and disturbed expression of pectin catabolism
CC family genes are observed (PubMed:22108525, PubMed:25039074). In
CC addition, fruits are smaller, but in a slightly higher number, with a
CC longer shelf life; they are firmer and more flexible during the
CC ripening stages after the mature green stage with considerably fewer
CC seeds, greater viscosity of juices, higher ethylene release and a deep
CC red color (PubMed:22108525). Silenced plants fruits have a
CC slow/suppressed ripening with abnormal orange color and associated with
CC lower ABA levels. In fruits exposed to water deprivation, water loss of
CC the sepal is enhanced (PubMed:25039074). {ECO:0000269|PubMed:22108525,
CC ECO:0000269|PubMed:22345638, ECO:0000269|PubMed:25039074,
CC ECO:0000269|PubMed:29632966, ECO:0000269|Ref.2}.
CC -!- BIOTECHNOLOGY: Interfering with NCED1 expression in fruits increases
CC shelf life and reduces seed number, but increases viscosity of juices
CC and modifies fruit texture. {ECO:0000269|PubMed:22108525}.
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family. {ECO:0000305}.
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DR EMBL; Z97215; CAB10168.1; -; mRNA.
DR EMBL; AJ439079; CAD30202.1; -; Genomic_DNA.
DR EMBL; CM001070; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; T07123; T07123.
DR RefSeq; NP_001234455.1; NM_001247526.2.
DR AlphaFoldDB; O24023; -.
DR SMR; O24023; -.
DR STRING; 4081.Solyc07g056570.1.1; -.
DR PaxDb; O24023; -.
DR PRIDE; O24023; -.
DR EnsemblPlants; Solyc07g056570.1.1; Solyc07g056570.1.1.1; Solyc07g056570.1.
DR GeneID; 544163; -.
DR Gramene; Solyc07g056570.1.1; Solyc07g056570.1.1.1; Solyc07g056570.1.
DR KEGG; sly:544163; -.
DR eggNOG; KOG1285; Eukaryota.
DR HOGENOM; CLU_016472_0_0_1; -.
DR InParanoid; O24023; -.
DR OMA; SYRNRWV; -.
DR OrthoDB; 524712at2759; -.
DR PhylomeDB; O24023; -.
DR UniPathway; UPA00090; -.
DR Proteomes; UP000004994; Chromosome 7.
DR GO; GO:0009570; C:chloroplast stroma; IBA:GO_Central.
DR GO; GO:0045549; F:9-cis-epoxycarotenoid dioxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0010436; F:carotenoid dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009688; P:abscisic acid biosynthetic process; IMP:UniProtKB.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0048653; P:anther development; IMP:UniProtKB.
DR GO; GO:1901812; P:beta-carotene biosynthetic process; IMP:UniProtKB.
DR GO; GO:0016121; P:carotene catabolic process; IBA:GO_Central.
DR GO; GO:0009835; P:fruit ripening; IMP:UniProtKB.
DR GO; GO:1901177; P:lycopene biosynthetic process; IMP:UniProtKB.
DR GO; GO:0009555; P:pollen development; IMP:UniProtKB.
DR GO; GO:0009856; P:pollination; IEP:UniProtKB.
DR GO; GO:0009737; P:response to abscisic acid; IEP:UniProtKB.
DR GO; GO:0009733; P:response to auxin; IEP:UniProtKB.
DR GO; GO:0009739; P:response to gibberellin; IEP:UniProtKB.
DR GO; GO:0009751; P:response to salicylic acid; IEP:UniProtKB.
DR GO; GO:0009651; P:response to salt stress; IEP:UniProtKB.
DR GO; GO:0009414; P:response to water deprivation; IMP:UniProtKB.
DR GO; GO:0016123; P:xanthophyll biosynthetic process; IMP:UniProtKB.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543; PTHR10543; 1.
DR Pfam; PF03055; RPE65; 1.
PE 1: Evidence at protein level;
KW Abscisic acid biosynthesis; Abscisic acid signaling pathway; Chloroplast;
KW Developmental protein; Dioxygenase; Fruit ripening; Iron; Metal-binding;
KW Oxidoreductase; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..16
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 17..605
FT /note="9-cis-epoxycarotenoid dioxygenase NCED1,
FT chloroplastic"
FT /id="PRO_0000445687"
FT REGION 55..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 302
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 351
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 416
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 592
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
SQ SEQUENCE 605 AA; 67317 MW; 19BA67930346A872 CRC64;
MATTTSHATN TWIKTKLSMP SSKEFGFASN SISLLKNQHN RQSLNINSSL QAPPILHFPK
QSSNYQTPKN NTISHPKQEN NNSSSSSTSK WNLVQKAAAM ALDAVESALT KHELEHPLPK
TADPRVQISG NFAPVPENPV CQSLPVTGKI PKCVQGVYVR NGANPLFEPT AGHHFFDGDG
MVHAVQFKNG SASYACRFTE TERLVQEKAL GRPVFPKAIG ELHGHSGIAR LMLFYARGLF
GLVDHSKGTG VANAGLVYFN NRLLAMSEDD LPYHVKVTPT GDLKTEGRFD FDGQLKSTMI
AHPKLDPVSG ELFALSYDVI QKPYLKYFRF SKNGEKSNDV EIPVEDPTMM HDFAITENFV
VIPDQQVVFK MSEMIRGGSP VVYDKNKVSR FGILDKYAKD GSDLKWVEVP DCFCFHLWNA
WEEAETDEIV VIGSCMTPPD SIFNECDEGL KSVLSEIRLN LKTGKSTRKS IIENPDEQVN
LEAGMVNRNK LGRKTEYAYL AIAEPWPKVS GFAKVNLFTG EVEKFIYGDN KYGGEPLFLP
RDPNSKEEDD GYILAFVHDE KEWKSELQIV NAMSLKLEAT VKLPSRVPYG FHGTFINAND
LANQA
