NCED2_ORYSJ
ID NCED2_ORYSJ Reviewed; 576 AA.
AC Q5MBR6;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic {ECO:0000305};
DE Short=OsNCED2 {ECO:0000312|EMBL:AAW21318.1};
DE EC=1.13.11.51 {ECO:0000250|UniProtKB:O24592};
DE Flags: Precursor;
GN Name=NCED2 {ECO:0000312|EMBL:AAW21318.1};
GN OrderedLocusNames=Os12g0435200 {ECO:0000312|EMBL:BAF29705.1},
GN LOC_Os12g24800 {ECO:0000312|EMBL:ABA97976.1};
GN ORFNames=OsJ_35952 {ECO:0000312|EMBL:EAZ20344.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Dian W.M.;
RT "Oryza sativa japonica group 9-cis-epoxycarotenoid dioxygenase 2 (NCED2)
RT mRNA.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP INDUCTION.
RX PubMed=17205969; DOI=10.1093/pcp/pcm003;
RA Saika H., Okamoto M., Miyoshi K., Kushiro T., Shinoda S., Jikumaru Y.,
RA Fujimoto M., Arikawa T., Takahashi H., Ando M., Arimura S., Miyao A.,
RA Hirochika H., Kamiya Y., Tsutsumi N., Nambara E., Nakazono M.;
RT "Ethylene promotes submergence-induced expression of OsABA8ox1, a gene that
RT encodes ABA 8'-hydroxylase in rice.";
RL Plant Cell Physiol. 48:287-298(2007).
RN [8]
RP INDUCTION.
RX PubMed=22859682; DOI=10.1093/jxb/ers206;
RA Song S., Dai X., Zhang W.H.;
RT "A rice F-box gene, OsFbx352, is involved in glucose-delayed seed
RT germination in rice.";
RL J. Exp. Bot. 63:5559-5568(2012).
RN [9]
RP INDUCTION BY DROUGHT STRESS.
RX PubMed=25735958; DOI=10.1093/pcp/pcv022;
RA Shi L., Guo M., Ye N., Liu Y., Liu R., Xia Y., Cui S., Zhang J.;
RT "Reduced ABA accumulation in the root system is caused by ABA exudation in
RT upland rice (Oryza sativa L. var. Gaoshan1) and this enhanced drought
RT adaptation.";
RL Plant Cell Physiol. 56:951-964(2015).
CC -!- FUNCTION: Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity.
CC Catalyzes the first step of abscisic-acid biosynthesis from
CC carotenoids. {ECO:0000250|UniProtKB:O24592}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a
CC 12'-apo-carotenal; Xref=Rhea:RHEA:23328, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:51972, ChEBI:CHEBI:51973;
CC EC=1.13.11.51; Evidence={ECO:0000250|UniProtKB:O24592};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-
CC dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-xanthoxin;
CC Xref=Rhea:RHEA:16541, ChEBI:CHEBI:15379, ChEBI:CHEBI:32304,
CC ChEBI:CHEBI:34597, ChEBI:CHEBI:35305; EC=1.13.11.51;
CC Evidence={ECO:0000250|UniProtKB:O24592};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-
CC didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-
CC xanthoxin; Xref=Rhea:RHEA:19677, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:34596, ChEBI:CHEBI:35306;
CC EC=1.13.11.51; Evidence={ECO:0000250|UniProtKB:O24592};
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000250|UniProtKB:O24592};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000250|UniProtKB:O24592};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- INDUCTION: Down-regulated by submergence (PubMed:17205969). Induced by
CC drought stress (PubMed:25735958). Down-regulated by glucose
CC (PubMed:22859682). {ECO:0000269|PubMed:17205969,
CC ECO:0000269|PubMed:22859682, ECO:0000269|PubMed:25735958}.
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family. {ECO:0000305}.
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DR EMBL; AY838898; AAW21318.1; -; mRNA.
DR EMBL; DP000011; ABA97976.1; -; Genomic_DNA.
DR EMBL; AP008218; BAF29705.1; -; Genomic_DNA.
DR EMBL; AP014968; BAT16965.1; -; Genomic_DNA.
DR EMBL; CM000149; EAZ20344.1; -; Genomic_DNA.
DR RefSeq; XP_015619611.1; XM_015764125.1.
DR AlphaFoldDB; Q5MBR6; -.
DR SMR; Q5MBR6; -.
DR STRING; 4530.OS12T0435200-01; -.
DR PaxDb; Q5MBR6; -.
DR PRIDE; Q5MBR6; -.
DR EnsemblPlants; Os12t0435200-01; Os12t0435200-01; Os12g0435200.
DR GeneID; 4352125; -.
DR Gramene; Os12t0435200-01; Os12t0435200-01; Os12g0435200.
DR KEGG; osa:4352125; -.
DR eggNOG; KOG1285; Eukaryota.
DR HOGENOM; CLU_016472_0_0_1; -.
DR InParanoid; Q5MBR6; -.
DR OMA; RINLCTG; -.
DR OrthoDB; 524712at2759; -.
DR Proteomes; UP000000763; Chromosome 12.
DR Proteomes; UP000007752; Chromosome 12.
DR Proteomes; UP000059680; Chromosome 12.
DR GO; GO:0009570; C:chloroplast stroma; IBA:GO_Central.
DR GO; GO:0045549; F:9-cis-epoxycarotenoid dioxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0010436; F:carotenoid dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009688; P:abscisic acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0016121; P:carotene catabolic process; IBA:GO_Central.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543; PTHR10543; 1.
DR Pfam; PF03055; RPE65; 1.
PE 2: Evidence at transcript level;
KW Abscisic acid biosynthesis; Chloroplast; Dioxygenase; Iron; Metal-binding;
KW Oxidoreductase; Plastid; Reference proteome; Stress response;
KW Transit peptide.
FT TRANSIT 1..34
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 35..576
FT /note="9-cis-epoxycarotenoid dioxygenase NCED2,
FT chloroplastic"
FT /id="PRO_0000440938"
FT BINDING 270
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 319
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 385
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 563
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
SQ SEQUENCE 576 AA; 63868 MW; 155A61B3684BBA45 CRC64;
MEVPIAAMTF AHPANVMTLA SRQPKSKRSH ISPATTAHRN LQTRLAHHHH ATPASLPMAI
CNTVDKVINR FIDLPEQRPT VDPRRVLSGN FAPVDELPPT SCHVIRGSIP SCLAGGVYIR
NGPNPQHRLP QRTHHLFDGD GMLHSLLIPS ASSTLLSEPV LCSRYVHTYK YLLERETGGP
VLPNFFAGFH GVAGLARAVV MIARVLAGQI NLNKGFGLAN TSITLFADCL YALCESDLPY
SMHINPANGE VTTLGRCDFG GDLSFRMTAH PKKDPVTMEL FAFRYNVFQP FITYFWFDRA
GSKVADVPIL SLQKPSVMHD FAITERYAIF PESQLIVNPM DMVMRGSSLV GLDRTMVPRI
GVLPRYAKDE SDMRWFEVPR FNMLHTTNGW EEADGEEIVL VAPNILSIEH MLGNMELMRA
RVDMVRINLC TGDVSCTALS PESLEFGVIH QGYVGRKNRY GYFGVSGPLP KIKGIRKLDF
DLVGSGDCTV GRRDFGLGCF AGEPFFVPDN IDGYGNEDSG YVVCYTHEED TGESWFVVMD
AKSPELDIVA EVQLPSRIPY GFHGIFVKQA ELLAQQ
