NCED2_SOLLC
ID NCED2_SOLLC Reviewed; 581 AA.
AC K4CJJ1; C5H805;
DT 07-NOV-2018, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2012, sequence version 1.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic {ECO:0000303|PubMed:19246595};
DE Short=LeNCED2 {ECO:0000303|PubMed:19246595};
DE Short=SlNCED2 {ECO:0000303|PubMed:22345638};
DE EC=1.13.11.51 {ECO:0000250|UniProtKB:Q9LRR7};
DE AltName: Full=Nine-cis-epoxycarotenoid dioxygenase 2 {ECO:0000303|PubMed:19246595};
DE Flags: Precursor;
GN Name=NCED2 {ECO:0000303|PubMed:19246595};
GN OrderedLocusNames=Solyc08g016720.1.1 {ECO:0000305};
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Heinz 1706;
RX PubMed=22660326; DOI=10.1038/nature11119;
RG Tomato Genome Consortium;
RT "The tomato genome sequence provides insights into fleshy fruit
RT evolution.";
RL Nature 485:635-641(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE OF 153-581.
RC STRAIN=cv. Ailsa Craig; TISSUE=Fruit;
RX PubMed=19246595; DOI=10.1093/jxb/erp026;
RA Zhang M., Yuan B., Leng P.;
RT "The role of ABA in triggering ethylene biosynthesis and ripening of tomato
RT fruit.";
RL J. Exp. Bot. 60:1579-1588(2009).
RN [3]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Jia Bao;
RX PubMed=22345638; DOI=10.1093/jxb/ers026;
RA Sun L., Yuan B., Zhang M., Wang L., Cui M., Wang Q., Leng P.;
RT "Fruit-specific RNAi-mediated suppression of SlNCED1 increases both
RT lycopene and beta-carotene contents in tomato fruit.";
RL J. Exp. Bot. 63:3097-3108(2012).
RN [4]
RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Jia Bao;
RX PubMed=22108525; DOI=10.1104/pp.111.186866;
RA Sun L., Sun Y., Zhang M., Wang L., Ren J., Cui M., Wang Y., Ji K., Li P.,
RA Li Q., Chen P., Dai S., Duan C., Wu Y., Leng P.;
RT "Suppression of 9-cis-epoxycarotenoid dioxygenase, which encodes a key
RT enzyme in abscisic acid biosynthesis, alters fruit texture in transgenic
RT tomato.";
RL Plant Physiol. 158:283-298(2012).
RN [5]
RP INDUCTION BY ABSCISIC-ACID AND DEHYDRATION, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Jia Bao;
RX PubMed=25039074; DOI=10.1093/jxb/eru288;
RA Ji K., Kai W., Zhao B., Sun Y., Yuan B., Dai S., Li Q., Chen P., Wang Y.,
RA Pei Y., Wang H., Guo Y., Leng P.;
RT "SlNCED1 and SlCYP707A2: key genes involved in ABA metabolism during tomato
RT fruit ripening.";
RL J. Exp. Bot. 65:5243-5255(2014).
RN [6]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Jia Bao;
RX PubMed=29632966; DOI=10.1007/s00018-018-2809-9;
RA Dai S., Kai W., Liang B., Wang J., Jiang L., Du Y., Sun Y., Leng P.;
RT "The functional analysis of SlNCED1 in tomato pollen development.";
RL Cell. Mol. Life Sci. 75:3457-3472(2018).
RN [7]
RP XXX, FUNCTION, AND INDUCTION BY LEAF SENESCENCE.
RX PubMed=29760199; DOI=10.1104/pp.18.00292;
RA Ma X., Zhang Y., Tureckova V., Xue G.-P., Fernie A.R., Mueller-Roeber B.,
RA Balazadeh S.;
RT "The NAC transcription factor SlNAP2 regulates leaf senescence and fruit
RT yield in tomato.";
RL Plant Physiol. 177:1286-1302(2018).
CC -!- FUNCTION: Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity.
CC Catalyzes the first step of abscisic-acid (ABA) biosynthesis from
CC carotenoids. {ECO:0000250|UniProtKB:O24023}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a
CC 12'-apo-carotenal; Xref=Rhea:RHEA:23328, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:51972, ChEBI:CHEBI:51973;
CC EC=1.13.11.51; Evidence={ECO:0000250|UniProtKB:Q9LRR7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-
CC dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-xanthoxin;
CC Xref=Rhea:RHEA:16541, ChEBI:CHEBI:15379, ChEBI:CHEBI:32304,
CC ChEBI:CHEBI:34597, ChEBI:CHEBI:35305; EC=1.13.11.51;
CC Evidence={ECO:0000250|UniProtKB:Q9LRR7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-
CC didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-
CC xanthoxin; Xref=Rhea:RHEA:19677, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:34596, ChEBI:CHEBI:35306;
CC EC=1.13.11.51; Evidence={ECO:0000250|UniProtKB:Q9LRR7};
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000250|UniProtKB:O24592};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000250|UniProtKB:O24592};
CC -!- PATHWAY: Plant hormone biosynthesis; abscisate biosynthesis.
CC {ECO:0000250|UniProtKB:O24023}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC {ECO:0000250|UniProtKB:Q9LRR7}. Note=Partially bound to the thylakoid.
CC {ECO:0000250|UniProtKB:Q9LRR7}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers (PubMed:29632966). Expressed
CC in developing and ripening fruits (PubMed:22345638, PubMed:22108525).
CC {ECO:0000269|PubMed:22108525, ECO:0000269|PubMed:22345638,
CC ECO:0000269|PubMed:29632966}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the meristem during gametogenesis and
CC mainly in ovule, stigma, anther/pollen and vascular tissues during
CC flower development. In flower buds, present in petal primordium and
CC apical meristem. Later expressed in stamen primordium and pistil
CC meristem after petal formation. Accumulates in stamen, pistil and
CC vascular tissues of the bud base. Highly expressed in sporogenous cells
CC in anther, pistil stigma, ovules, pollen grains, anther walls, and
CC basal vascular bundle. In anthers, first present at high levels, then
CC declines rapidly from stage 13 (when microspores transform into
CC vacuolated pollen grains after mitotic divisions) to stage 16 (when the
CC sepals are better separated, and the faint yellow petals are clearer)
CC to a very low level (PubMed:29632966). In fruits, expressed at high
CC levels at the immature stage and then declined continually through all
CC stages of fruit development (PubMed:22345638, PubMed:22108525,
CC PubMed:25039074). {ECO:0000269|PubMed:22108525,
CC ECO:0000269|PubMed:22345638, ECO:0000269|PubMed:25039074,
CC ECO:0000269|PubMed:29632966}.
CC -!- INDUCTION: Induced by abscisic-acid (ABA) and dehydration.
CC {ECO:0000269|PubMed:25039074}.
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family. {ECO:0000305}.
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DR EMBL; CM001071; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; EU912387; ACL00683.2; -; mRNA.
DR RefSeq; XP_004244807.1; XM_004244759.3.
DR AlphaFoldDB; K4CJJ1; -.
DR SMR; K4CJJ1; -.
DR STRING; 4081.Solyc08g016720.1.1; -.
DR PaxDb; K4CJJ1; -.
DR PRIDE; K4CJJ1; -.
DR EnsemblPlants; Solyc08g016720.1.1; Solyc08g016720.1.1.1; Solyc08g016720.1.
DR GeneID; 100316877; -.
DR Gramene; Solyc08g016720.1.1; Solyc08g016720.1.1.1; Solyc08g016720.1.
DR KEGG; sly:100316877; -.
DR eggNOG; KOG1285; Eukaryota.
DR HOGENOM; CLU_016472_0_0_1; -.
DR InParanoid; K4CJJ1; -.
DR OMA; KMFPEAG; -.
DR OrthoDB; 524712at2759; -.
DR PhylomeDB; K4CJJ1; -.
DR BRENDA; 1.13.11.51; 3101.
DR UniPathway; UPA00090; -.
DR Proteomes; UP000004994; Chromosome 8.
DR ExpressionAtlas; K4CJJ1; baseline and differential.
DR GO; GO:0009570; C:chloroplast stroma; IBA:GO_Central.
DR GO; GO:0045549; F:9-cis-epoxycarotenoid dioxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0010436; F:carotenoid dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009688; P:abscisic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0016121; P:carotene catabolic process; IBA:GO_Central.
DR GO; GO:0009737; P:response to abscisic acid; IEP:UniProtKB.
DR GO; GO:0009414; P:response to water deprivation; IEP:UniProtKB.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543; PTHR10543; 1.
DR Pfam; PF03055; RPE65; 1.
PE 2: Evidence at transcript level;
KW Abscisic acid biosynthesis; Chloroplast; Dioxygenase; Iron; Metal-binding;
KW Oxidoreductase; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..82
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 83..581
FT /note="9-cis-epoxycarotenoid dioxygenase NCED2,
FT chloroplastic"
FT /id="PRO_0000445688"
FT BINDING 279
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 328
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 393
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 568
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT CONFLICT 335
FT /note="N -> S (in Ref. 2; ACL00683)"
FT /evidence="ECO:0000305"
FT CONFLICT 449
FT /note="L -> P (in Ref. 2; ACL00683)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 581 AA; 64834 MW; 8E9F510AC75D666C CRC64;
MTSTIANYRV SHSFSPSTSY SLDFTLPSKS ISMKNHTTTK TKIHSALLTL PKQNNTPKNQ
PQFQTSHWNF FQKAAAKALD IVESALVSRE LQNPLPKTAD PRVQIAGNFA PVPEQSVRHN
LPVTGTIPDC INGVYVRNGA NPLFEPVAGH HLFDGDGMVH AVTVENGSVS YSCRFTETER
LVQERELGHP VFPKAIGELH GHSGIARLLL FYARGVFGLV DHSHGTGVAN AGLVFFNNRL
LAMSEDDVPY HVQVLPSGDL QTVGRYNFDD QLKSTMIAHP KIDPVSGELF ALSYDVVQKP
YLKSFKFSPD GEKSPDVEIP LDVPTMMHDF AITENYVVIP DQQVVFKLQE MIKGGSPVIY
DKNKKSRFGI LPKNAENSEN IIWVESAETF CFHLWNAWEE PETDEVIVIG SCMTPPDSIF
NECNENLKSV LSEIRLNLKT GESTRRQLLS PSDQVNLEAG MVNRNKLGRK TQFAYLAIAE
PWPKVSGFAK VDLSTGEIKK HIYGDKRYGG EPLFLPRNVN SEKEDDGYIL AFCHDEKTWK
SELQIVNAMT LELEATVKLP SRVPYGFHGT FISSKDLQNQ V
