NCED3_ARATH
ID NCED3_ARATH Reviewed; 599 AA.
AC Q9LRR7; Q93ZU5;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic {ECO:0000303|PubMed:11532178};
DE Short=AtNCED3 {ECO:0000303|PubMed:11532178};
DE EC=1.13.11.51 {ECO:0000269|PubMed:11532178, ECO:0000269|PubMed:15466233};
DE AltName: Full=Protein SALT TOLERANT 1 {ECO:0000303|PubMed:15466233};
DE Flags: Precursor;
GN Name=NCED3 {ECO:0000303|PubMed:11532178};
GN Synonyms=STO1 {ECO:0000303|PubMed:15466233};
GN OrderedLocusNames=At3g14440 {ECO:0000312|Araport:AT3G14440};
GN ORFNames=MOA2.4 {ECO:0000312|EMBL:BAB01336.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION BY DROUGHT STRESS,
RP DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=11532178; DOI=10.1046/j.1365-313x.2001.01096.x;
RA Iuchi S., Kobayashi M., Taji T., Naramoto M., Seki M., Kato T., Tabata S.,
RA Kakubari Y., Yamaguchi-Shinozaki K., Shinozaki K.;
RT "Regulation of drought tolerance by gene manipulation of 9-cis-
RT epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in
RT Arabidopsis.";
RL Plant J. 27:325-333(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY DROUGHT STRESS.
RX PubMed=12834401; DOI=10.1046/j.1365-313x.2003.01786.x;
RA Tan B.-C., Joseph L.M., Deng W.-T., Liu L., Li Q.-B., Cline K.,
RA McCarty D.R.;
RT "Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid
RT dioxygenase gene family.";
RL Plant J. 35:44-56(2003).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION BY SALT STRESS, CATALYTIC ACTIVITY,
RP AND PATHWAY.
RX PubMed=15466233; DOI=10.1104/pp.104.046169;
RA Ruggiero B., Koiwa H., Manabe Y., Quist T.M., Inan G., Saccardo F.,
RA Joly R.J., Hasegawa P.M., Bressan R.A., Maggio A.;
RT "Uncoupling the effects of abscisic acid on plant growth and water
RT relations. Analysis of sto1/nced3, an abscisic acid-deficient but salt
RT stress-tolerant mutant in Arabidopsis.";
RL Plant Physiol. 136:3134-3147(2004).
RN [7]
RP ACTIVITY REGULATION.
RX PubMed=15247398; DOI=10.1104/pp.104.039511;
RA Han S.Y., Kitahata N., Sekimata K., Saito T., Kobayashi M., Nakashima K.,
RA Yamaguchi-Shinozaki K., Shinozaki K., Yoshida S., Asami T.;
RT "A novel inhibitor of 9-cis-epoxycarotenoid dioxygenase in abscisic acid
RT biosynthesis in higher plants.";
RL Plant Physiol. 135:1574-1582(2004).
RN [8]
RP ACTIVITY REGULATION.
RX PubMed=16682205; DOI=10.1016/j.bmc.2006.04.025;
RA Kitahata N., Han S.Y., Noji N., Saito T., Kobayashi M., Nakano T.,
RA Kuchitsu K., Shinozaki K., Yoshida S., Matsumoto S., Tsujimoto M.,
RA Asami T.;
RT "A 9-cis-epoxycarotenoid dioxygenase inhibitor for use in the elucidation
RT of abscisic acid action mechanisms.";
RL Bioorg. Med. Chem. 14:5555-5561(2006).
RN [9]
RP TISSUE SPECIFICITY.
RX PubMed=16412079; DOI=10.1111/j.1365-313x.2005.02622.x;
RA Lefebvre V., North H., Frey A., Sotta B., Seo M., Okamoto M., Nambara E.,
RA Marion-Poll A.;
RT "Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that
RT ABA synthesized in the endosperm is involved in the induction of seed
RT dormancy.";
RL Plant J. 45:309-319(2006).
RN [10]
RP INDUCTION BY PATHOGEN.
RX PubMed=17304219; DOI=10.1038/sj.emboj.7601575;
RA de Torres-Zabala M., Truman W., Bennett M.H., Lafforgue G., Mansfield J.W.,
RA Rodriguez Egea P., Boegre L., Grant M.;
RT "Pseudomonas syringae pv. tomato hijacks the Arabidopsis abscisic acid
RT signalling pathway to cause disease.";
RL EMBO J. 26:1434-1443(2007).
CC -!- FUNCTION: Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity.
CC Catalyzes the first step of abscisic-acid biosynthesis from
CC carotenoids, in response to water stress. {ECO:0000269|PubMed:11532178,
CC ECO:0000269|PubMed:15466233}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a
CC 12'-apo-carotenal; Xref=Rhea:RHEA:23328, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:51972, ChEBI:CHEBI:51973;
CC EC=1.13.11.51; Evidence={ECO:0000269|PubMed:11532178,
CC ECO:0000269|PubMed:15466233};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-
CC dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-xanthoxin;
CC Xref=Rhea:RHEA:16541, ChEBI:CHEBI:15379, ChEBI:CHEBI:32304,
CC ChEBI:CHEBI:34597, ChEBI:CHEBI:35305; EC=1.13.11.51;
CC Evidence={ECO:0000269|PubMed:11532178, ECO:0000269|PubMed:15466233};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-
CC didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-
CC xanthoxin; Xref=Rhea:RHEA:19677, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:34596, ChEBI:CHEBI:35306;
CC EC=1.13.11.51; Evidence={ECO:0000269|PubMed:11532178,
CC ECO:0000269|PubMed:15466233};
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000250|UniProtKB:O24592};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000250|UniProtKB:O24592};
CC -!- ACTIVITY REGULATION: Inhibited by abamine and abamineSG.
CC {ECO:0000269|PubMed:15247398, ECO:0000269|PubMed:16682205}.
CC -!- PATHWAY: Plant hormone biosynthesis; abscisate biosynthesis.
CC {ECO:0000269|PubMed:11532178, ECO:0000269|PubMed:15466233}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC {ECO:0000269|PubMed:12834401}. Note=Partially bound to the thylakoid.
CC -!- TISSUE SPECIFICITY: Localized in roots, leaves, stems, empty silique
CC envelopes and seeds. Expressed at the point of organ attachment and the
CC abscission zones in the plant. {ECO:0000269|PubMed:12834401,
CC ECO:0000269|PubMed:15466233, ECO:0000269|PubMed:16412079}.
CC -!- INDUCTION: By salt or drought stress and pathogen.
CC {ECO:0000269|PubMed:11532178, ECO:0000269|PubMed:12834401,
CC ECO:0000269|PubMed:15466233, ECO:0000269|PubMed:17304219}.
CC -!- DISRUPTION PHENOTYPE: Plants show enhanced germination on salt and
CC hypersensitivity to desiccation and LiCl.
CC {ECO:0000269|PubMed:11532178}.
CC -!- MISCELLANEOUS: Overexpression of NCED3 results in increased
CC accumulation of abscisic acid and resistance to water stress.
CC {ECO:0000269|PubMed:15466233}.
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB026549; BAB70609.1; -; Genomic_DNA.
DR EMBL; AB028617; BAB01336.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75526.1; -; Genomic_DNA.
DR EMBL; AY056255; AAL07104.1; -; mRNA.
DR RefSeq; NP_188062.1; NM_112304.3.
DR AlphaFoldDB; Q9LRR7; -.
DR SMR; Q9LRR7; -.
DR BioGRID; 6001; 1.
DR STRING; 3702.AT3G14440.1; -.
DR PaxDb; Q9LRR7; -.
DR PRIDE; Q9LRR7; -.
DR ProteomicsDB; 251317; -.
DR EnsemblPlants; AT3G14440.1; AT3G14440.1; AT3G14440.
DR GeneID; 820667; -.
DR Gramene; AT3G14440.1; AT3G14440.1; AT3G14440.
DR KEGG; ath:AT3G14440; -.
DR Araport; AT3G14440; -.
DR TAIR; locus:2091652; AT3G14440.
DR eggNOG; KOG1285; Eukaryota.
DR HOGENOM; CLU_016472_0_0_1; -.
DR InParanoid; Q9LRR7; -.
DR OMA; SYRNRWV; -.
DR OrthoDB; 524712at2759; -.
DR PhylomeDB; Q9LRR7; -.
DR BioCyc; ARA:AT3G14440-MON; -.
DR BioCyc; MetaCyc:AT3G14440-MON; -.
DR BRENDA; 1.13.11.51; 399.
DR UniPathway; UPA00090; -.
DR PRO; PR:Q9LRR7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LRR7; baseline and differential.
DR Genevisible; Q9LRR7; AT.
DR GO; GO:0009570; C:chloroplast stroma; IDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:TAIR.
DR GO; GO:0045549; F:9-cis-epoxycarotenoid dioxygenase activity; IDA:TAIR.
DR GO; GO:0010436; F:carotenoid dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009688; P:abscisic acid biosynthetic process; IMP:TAIR.
DR GO; GO:0016121; P:carotene catabolic process; IBA:GO_Central.
DR GO; GO:0042538; P:hyperosmotic salinity response; IMP:TAIR.
DR GO; GO:0006970; P:response to osmotic stress; IMP:TAIR.
DR GO; GO:0009414; P:response to water deprivation; IMP:TAIR.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543; PTHR10543; 1.
DR Pfam; PF03055; RPE65; 1.
PE 1: Evidence at protein level;
KW Abscisic acid biosynthesis; Chloroplast; Dioxygenase; Iron; Metal-binding;
KW Oxidoreductase; Plastid; Reference proteome; Stress response;
KW Transit peptide.
FT TRANSIT 1..40
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 41..599
FT /note="9-cis-epoxycarotenoid dioxygenase NCED3,
FT chloroplastic"
FT /id="PRO_0000285991"
FT BINDING 297
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 346
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 411
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 585
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT CONFLICT 371
FT /note="R -> L (in Ref. 4; AAL07104)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 599 AA; 65857 MW; 7D513F39945E0CF3 CRC64;
MASFTATAAV SGRWLGGNHT QPPLSSSQSS DLSYCSSLPM ASRVTRKLNV SSALHTPPAL
HFPKQSSNSP AIVVKPKAKE SNTKQMNLFQ RAAAAALDAA EGFLVSHEKL HPLPKTADPS
VQIAGNFAPV NEQPVRRNLP VVGKLPDSIK GVYVRNGANP LHEPVTGHHF FDGDGMVHAV
KFEHGSASYA CRFTQTNRFV QERQLGRPVF PKAIGELHGH TGIARLMLFY ARAAAGIVDP
AHGTGVANAG LVYFNGRLLA MSEDDLPYQV QITPNGDLKT VGRFDFDGQL ESTMIAHPKV
DPESGELFAL SYDVVSKPYL KYFRFSPDGT KSPDVEIQLD QPTMMHDFAI TENFVVVPDQ
QVVFKLPEMI RGGSPVVYDK NKVARFGILD KYAEDSSNIK WIDAPDCFCF HLWNAWEEPE
TDEVVVIGSC MTPPDSIFNE SDENLKSVLS EIRLNLKTGE STRRPIISNE DQQVNLEAGM
VNRNMLGRKT KFAYLALAEP WPKVSGFAKV DLTTGEVKKH LYGDNRYGGE PLFLPGEGGE
EDEGYILCFV HDEKTWKSEL QIVNAVSLEV EATVKLPSRV PYGFHGTFIG ADDLAKQVV
