NCED4_ORYSJ
ID NCED4_ORYSJ Reviewed; 582 AA.
AC Q69NX5; Q0D8J6;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED4, chloroplastic {ECO:0000305};
DE Short=OsNCED4 {ECO:0000312|EMBL:AAW21320.1};
DE EC=1.13.11.51 {ECO:0000250|UniProtKB:O24592};
DE Flags: Precursor;
GN Name=NCED4 {ECO:0000312|EMBL:AAW21320.1};
GN OrderedLocusNames=Os07g0154100 {ECO:0000312|EMBL:BAT00112.1},
GN LOC_Os07g05940 {ECO:0000305};
GN ORFNames=OsJ_23132 {ECO:0000312|EMBL:EAZ38731.1},
GN OSJNBb0050B07.21 {ECO:0000312|EMBL:BAD31592.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Dian W.M.;
RT "Oryza sativa japonica group 9-cis-epoxycarotenoid dioxygenase 4 (NCED4)
RT mRNA.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP INDUCTION.
RX PubMed=18326788; DOI=10.1104/pp.108.117028;
RA Welsch R., Wuest F., Baer C., Al-Babili S., Beyer P.;
RT "A third phytoene synthase is devoted to abiotic stress-induced abscisic
RT acid formation in rice and defines functional diversification of phytoene
RT synthase genes.";
RL Plant Physiol. 147:367-380(2008).
RN [8]
RP INDUCTION BY D-ALLOSE.
RX PubMed=23397192; DOI=10.1007/s00425-013-1853-9;
RA Fukumoto T., Kano A., Ohtani K., Inoue M., Yoshihara A., Izumori K.,
RA Tajima S., Shigematsu Y., Tanaka K., Ohkouchi T., Ishida Y., Nishizawa Y.,
RA Tada Y., Ichimura K., Gomi K., Yoo S.D., Sheen J., Akimitsu K.;
RT "Phosphorylation of D-allose by hexokinase involved in regulation of OsABF1
RT expression for growth inhibition in Oryza sativa L.";
RL Planta 237:1379-1391(2013).
RN [9]
RP INDUCTION BY INFECTION WITH XOO.
RX PubMed=23826294; DOI=10.1371/journal.pone.0067413;
RA Xu J., Audenaert K., Hofte M., De Vleesschauwer D.;
RT "Abscisic acid promotes susceptibility to the rice leaf blight pathogen
RT Xanthomonas oryzae pv oryzae by suppressing salicylic acid-mediated
RT defenses.";
RL PLoS ONE 8:E67413-E67413(2013).
RN [10]
RP INDUCTION BY DROUGHT STRESS.
RX PubMed=25418692; DOI=10.1111/jipb.12313;
RA Du H., Chang Y., Huang F., Xiong L.;
RT "GID1 modulates stomatal response and submergence tolerance involving
RT abscisic acid and gibberellic acid signaling in rice.";
RL J. Integr. Plant Biol. 57:954-968(2015).
RN [11]
RP INDUCTION BY DROUGHT STRESS.
RX PubMed=25735958; DOI=10.1093/pcp/pcv022;
RA Shi L., Guo M., Ye N., Liu Y., Liu R., Xia Y., Cui S., Zhang J.;
RT "Reduced ABA accumulation in the root system is caused by ABA exudation in
RT upland rice (Oryza sativa L. var. Gaoshan1) and this enhanced drought
RT adaptation.";
RL Plant Cell Physiol. 56:951-964(2015).
CC -!- FUNCTION: Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity.
CC Catalyzes the first step of abscisic-acid biosynthesis from
CC carotenoids. {ECO:0000250|UniProtKB:O24592}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a
CC 12'-apo-carotenal; Xref=Rhea:RHEA:23328, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:51972, ChEBI:CHEBI:51973;
CC EC=1.13.11.51; Evidence={ECO:0000250|UniProtKB:O24592};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-
CC dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-xanthoxin;
CC Xref=Rhea:RHEA:16541, ChEBI:CHEBI:15379, ChEBI:CHEBI:32304,
CC ChEBI:CHEBI:34597, ChEBI:CHEBI:35305; EC=1.13.11.51;
CC Evidence={ECO:0000250|UniProtKB:O24592};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-
CC didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-
CC xanthoxin; Xref=Rhea:RHEA:19677, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:34596, ChEBI:CHEBI:35306;
CC EC=1.13.11.51; Evidence={ECO:0000250|UniProtKB:O24592};
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000250|UniProtKB:O24592};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000250|UniProtKB:O24592};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- INDUCTION: Induced by abscisic acid (ABA) and salt (PubMed:18326788).
CC Induced by D-allose (PubMed:23397192). Induced by infection with the
CC bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo)
CC (PubMed:23826294). Induced by drought stress (PubMed:25418692,
CC PubMed:25735958). {ECO:0000269|PubMed:18326788,
CC ECO:0000269|PubMed:23397192, ECO:0000269|PubMed:23826294,
CC ECO:0000269|PubMed:25418692, ECO:0000269|PubMed:25735958}.
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF20827.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AY838900; AAW21320.1; -; mRNA.
DR EMBL; AP005632; BAD31592.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF20827.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014963; BAT00112.1; -; Genomic_DNA.
DR EMBL; CM000144; EAZ38731.1; -; Genomic_DNA.
DR EMBL; AK119780; BAG99785.1; -; mRNA.
DR AlphaFoldDB; Q69NX5; -.
DR SMR; Q69NX5; -.
DR STRING; 4530.OS07T0154100-01; -.
DR PaxDb; Q69NX5; -.
DR PRIDE; Q69NX5; -.
DR EnsemblPlants; Os07t0154100-01; Os07t0154100-01; Os07g0154100.
DR Gramene; Os07t0154100-01; Os07t0154100-01; Os07g0154100.
DR eggNOG; KOG1285; Eukaryota.
DR HOGENOM; CLU_016472_0_0_1; -.
DR InParanoid; Q69NX5; -.
DR OMA; HDEERGT; -.
DR PlantReactome; R-OSA-1119374; Abscisic acid biosynthesis.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000007752; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR GO; GO:0009570; C:chloroplast stroma; IBA:GO_Central.
DR GO; GO:0045549; F:9-cis-epoxycarotenoid dioxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0010436; F:carotenoid dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009688; P:abscisic acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0016121; P:carotene catabolic process; IBA:GO_Central.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543; PTHR10543; 1.
DR Pfam; PF03055; RPE65; 1.
PE 2: Evidence at transcript level;
KW Abscisic acid biosynthesis; Chloroplast; Dioxygenase; Iron; Metal-binding;
KW Oxidoreductase; Plastid; Reference proteome; Stress response;
KW Transit peptide.
FT TRANSIT 1..64
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 65..582
FT /note="9-cis-epoxycarotenoid dioxygenase NCED4,
FT chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000440940"
FT REGION 1..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..25
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 273
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 322
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 387
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 569
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
SQ SEQUENCE 582 AA; 62948 MW; A3A79ACD01615B91 CRC64;
MASSAPSAPG LAPVAKPPPP PSKVKVATAT VPTNGKIKQG ARPMRVSAPP VEPRRRMNPL
QRLAAAAIDA VEEGLVAGLL ERGHALPRTA DPAVQIAGNY APVGERPPVR GLPVSGRLPA
CLDGVYVRNG ANPLHAPRAG HHLFDGDGML HAVRLAGGRA ESYACRFTET ARLRQEREMG
RPVFPKAIGE LHGHSGVARL LLFGSRALCG VLDASRGIGV ANAGLVYHDG RLLAMSEDDL
PYHVRVTHDG DLETVGRYDF HGQLDADGTM IAHPKLDPVT GELFALSYNV VSKPYLKYFY
FTADGRKSRD VDIPVGAPTM IHDFAVTENY AVVPDQQIVF KLQEMVRGGS PVVYDREKAS
RFGVLPKRAA DASELRWVEV PGCFCFHLWN AWEDDATGEI VVIGSCMTPP DAVFNEPSQS
PEEESFRSVL SEIRLDPRTG VSRRRDVLRD AAEQVNLEAG MVNRQLLGRK TRYAYLAIAE
PWPRVSGFAK VDLESGTAEK FIYGEGRYGG EPCFVPRAGA AAEDDGHVLC FVHDEERGTS
ELVVVDAGSE AMEEVAAVKL PGRVPYGLHG TFIGANELQR QA
