NCED5_ARATH
ID NCED5_ARATH Reviewed; 589 AA.
AC Q9C6Z1;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic;
DE Short=AtNCED5;
DE EC=1.13.11.51;
DE Flags: Precursor;
GN Name=NCED5; OrderedLocusNames=At1g30100; ORFNames=T2H7.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP INDUCTION BY DROUGHT STRESS.
RX PubMed=11532178; DOI=10.1046/j.1365-313x.2001.01096.x;
RA Iuchi S., Kobayashi M., Taji T., Naramoto M., Seki M., Kato T., Tabata S.,
RA Kakubari Y., Yamaguchi-Shinozaki K., Shinozaki K.;
RT "Regulation of drought tolerance by gene manipulation of 9-cis-
RT epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in
RT Arabidopsis.";
RL Plant J. 27:325-333(2001).
RN [5]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY DROUGHT STRESS.
RX PubMed=12834401; DOI=10.1046/j.1365-313x.2003.01786.x;
RA Tan B.-C., Joseph L.M., Deng W.-T., Liu L., Li Q.-B., Cline K.,
RA McCarty D.R.;
RT "Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid
RT dioxygenase gene family.";
RL Plant J. 35:44-56(2003).
RN [6]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH VAR3.
RX PubMed=15340011; DOI=10.1242/jcs.01360;
RA Naested H., Holm A., Jenkins T., Nielsen H.B., Harris C.A., Beale M.H.,
RA Andersen M., Mant A., Scheller H., Camara B., Mattsson O., Mundy J.;
RT "Arabidopsis VARIEGATED 3 encodes a chloroplast-targeted, zinc-finger
RT protein required for chloroplast and palisade cell development.";
RL J. Cell Sci. 117:4807-4818(2004).
RN [7]
RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX PubMed=16412079; DOI=10.1111/j.1365-313x.2005.02622.x;
RA Lefebvre V., North H., Frey A., Sotta B., Seo M., Okamoto M., Nambara E.,
RA Marion-Poll A.;
RT "Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that
RT ABA synthesized in the endosperm is involved in the induction of seed
RT dormancy.";
RL Plant J. 45:309-319(2006).
CC -!- FUNCTION: Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity.
CC Catalyzes the first step of abscisic-acid biosynthesis from carotenoids
CC (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a
CC 12'-apo-carotenal; Xref=Rhea:RHEA:23328, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:51972, ChEBI:CHEBI:51973;
CC EC=1.13.11.51;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-
CC dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-xanthoxin;
CC Xref=Rhea:RHEA:16541, ChEBI:CHEBI:15379, ChEBI:CHEBI:32304,
CC ChEBI:CHEBI:34597, ChEBI:CHEBI:35305; EC=1.13.11.51;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-
CC didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-
CC xanthoxin; Xref=Rhea:RHEA:19677, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:34596, ChEBI:CHEBI:35306;
CC EC=1.13.11.51;
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000250|UniProtKB:O24592};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000250|UniProtKB:O24592};
CC -!- SUBUNIT: Interacts in vitro with VAR3. {ECO:0000269|PubMed:15340011}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:12834401, ECO:0000269|PubMed:15340011}.
CC -!- TISSUE SPECIFICITY: Detected only in seeds.
CC {ECO:0000269|PubMed:12834401, ECO:0000269|PubMed:16412079}.
CC -!- DEVELOPMENTAL STAGE: Expressed at later stages of seed maturation.
CC {ECO:0000269|PubMed:16412079}.
CC -!- INDUCTION: Low induction by drought stress.
CC {ECO:0000269|PubMed:11532178, ECO:0000269|PubMed:12834401}.
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family. {ECO:0000305}.
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DR EMBL; AC074176; AAG50855.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31178.1; -; Genomic_DNA.
DR EMBL; AK226774; BAE98872.1; -; mRNA.
DR PIR; A86425; A86425.
DR RefSeq; NP_174302.1; NM_102749.3.
DR AlphaFoldDB; Q9C6Z1; -.
DR SMR; Q9C6Z1; -.
DR IntAct; Q9C6Z1; 1.
DR STRING; 3702.AT1G30100.1; -.
DR PaxDb; Q9C6Z1; -.
DR PRIDE; Q9C6Z1; -.
DR EnsemblPlants; AT1G30100.1; AT1G30100.1; AT1G30100.
DR GeneID; 839889; -.
DR Gramene; AT1G30100.1; AT1G30100.1; AT1G30100.
DR KEGG; ath:AT1G30100; -.
DR Araport; AT1G30100; -.
DR TAIR; locus:2202492; AT1G30100.
DR eggNOG; KOG1285; Eukaryota.
DR HOGENOM; CLU_016472_0_0_1; -.
DR InParanoid; Q9C6Z1; -.
DR OMA; KDTSHWN; -.
DR OrthoDB; 524712at2759; -.
DR PhylomeDB; Q9C6Z1; -.
DR BioCyc; ARA:AT1G30100-MON; -.
DR BRENDA; 1.13.11.51; 399.
DR PRO; PR:Q9C6Z1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C6Z1; baseline and differential.
DR Genevisible; Q9C6Z1; AT.
DR GO; GO:0009570; C:chloroplast stroma; IBA:GO_Central.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009579; C:thylakoid; IDA:TAIR.
DR GO; GO:0045549; F:9-cis-epoxycarotenoid dioxygenase activity; TAS:TAIR.
DR GO; GO:0010436; F:carotenoid dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009688; P:abscisic acid biosynthetic process; IMP:TAIR.
DR GO; GO:0016121; P:carotene catabolic process; IBA:GO_Central.
DR GO; GO:0009414; P:response to water deprivation; IGI:TAIR.
DR GO; GO:0010162; P:seed dormancy process; IGI:TAIR.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543; PTHR10543; 1.
DR Pfam; PF03055; RPE65; 1.
PE 1: Evidence at protein level;
KW Abscisic acid biosynthesis; Chloroplast; Dioxygenase; Iron; Membrane;
KW Metal-binding; Oxidoreductase; Plastid; Reference proteome; Thylakoid;
KW Transit peptide.
FT TRANSIT 1..45
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 46..589
FT /note="Probable 9-cis-epoxycarotenoid dioxygenase NCED5,
FT chloroplastic"
FT /id="PRO_0000285994"
FT REGION 21..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 287
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 336
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 401
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 576
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
SQ SEQUENCE 589 AA; 65337 MW; 6A79B2BF7EAA8179 CRC64;
MACSYILTPN PTKLNLSFAP SDLDAPSPSS SVSFTNTKPR RRKLSANSVS DTPNLLNFPN
YPSPNPIIPE KDTSRWNPLQ RAASAALDFA ETALLRRERS KPLPKTVDPR HQISGNYAPV
PEQSVKSSLS VDGKIPDCID GVYLRNGANP LFEPVSGHHL FDGDGMVHAV KITNGDASYS
CRFTETERLV QEKQLGSPIF PKAIGELHGH SGIARLMLFY ARGLFGLLNH KNGTGVANAG
LVYFHDRLLA MSEDDLPYQV RVTDNGDLET IGRFDFDGQL SSAMIAHPKI DPVTKELFAL
SYDVVKKPYL KYFKFSPEGE KSPDVEIPLA SPTMMHDFAI TENFVVIPDQ QVVFKLSDMF
LGKSPVKYDG EKISRFGILP RNAKDASEMV WVESPETFCF HLWNAWESPE TDEVVVIGSC
MTPADSIFNE CDEQLNSVLS EIRLNLKTGK STRRTIIPGS VQMNLEAGMV NRNLLGRKTR
YAYLAIAEPW PKVSGFAKVD LSTGEVKNHF YGGKKYGGEP FFLPRGLESD GEDDGYIMSF
VHDEESWESE LHIVNAVTLE LEATVKLPSR VPYGFHGTFV NSADMLNQA
