NCED6_ARATH
ID NCED6_ARATH Reviewed; 577 AA.
AC Q9LRM7;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic;
DE Short=AtNCED6;
DE EC=1.13.11.51;
DE Flags: Precursor;
GN Name=NCED6; OrderedLocusNames=At3g24220; ORFNames=MUJ8.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, INDUCTION BY DROUGHT STRESS, AND DISRUPTION PHENOTYPE.
RX PubMed=11532178; DOI=10.1046/j.1365-313x.2001.01096.x;
RA Iuchi S., Kobayashi M., Taji T., Naramoto M., Seki M., Kato T., Tabata S.,
RA Kakubari Y., Yamaguchi-Shinozaki K., Shinozaki K.;
RT "Regulation of drought tolerance by gene manipulation of 9-cis-
RT epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in
RT Arabidopsis.";
RL Plant J. 27:325-333(2001).
RN [4]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY DROUGHT STRESS.
RX PubMed=12834401; DOI=10.1046/j.1365-313x.2003.01786.x;
RA Tan B.-C., Joseph L.M., Deng W.-T., Liu L., Li Q.-B., Cline K.,
RA McCarty D.R.;
RT "Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid
RT dioxygenase gene family.";
RL Plant J. 35:44-56(2003).
RN [5]
RP FUNCTION, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX PubMed=16412079; DOI=10.1111/j.1365-313x.2005.02622.x;
RA Lefebvre V., North H., Frey A., Sotta B., Seo M., Okamoto M., Nambara E.,
RA Marion-Poll A.;
RT "Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that
RT ABA synthesized in the endosperm is involved in the induction of seed
RT dormancy.";
RL Plant J. 45:309-319(2006).
CC -!- FUNCTION: Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity.
CC Catalyzes the first step of abscisic-acid biosynthesis from
CC carotenoids. Contributes probably to abscisic acid synthesis for the
CC induction of seed dormancy. {ECO:0000269|PubMed:11532178,
CC ECO:0000269|PubMed:16412079}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a
CC 12'-apo-carotenal; Xref=Rhea:RHEA:23328, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:51972, ChEBI:CHEBI:51973;
CC EC=1.13.11.51;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-
CC dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-xanthoxin;
CC Xref=Rhea:RHEA:16541, ChEBI:CHEBI:15379, ChEBI:CHEBI:32304,
CC ChEBI:CHEBI:34597, ChEBI:CHEBI:35305; EC=1.13.11.51;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-
CC didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-
CC xanthoxin; Xref=Rhea:RHEA:19677, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:34596, ChEBI:CHEBI:35306;
CC EC=1.13.11.51;
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000250|UniProtKB:O24592};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000250|UniProtKB:O24592};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC {ECO:0000269|PubMed:12834401}. Note=Partially bound to the thylakoid.
CC -!- TISSUE SPECIFICITY: Expressed before fertilization in male and female
CC gametophytes, and then immediately after pollination, restricted to
CC seed endosperm. {ECO:0000269|PubMed:12834401,
CC ECO:0000269|PubMed:16412079}.
CC -!- DEVELOPMENTAL STAGE: Expressed in seeds at early and mid-maturation
CC stages. {ECO:0000269|PubMed:16412079}.
CC -!- INDUCTION: Low induction by drought stress.
CC {ECO:0000269|PubMed:11532178, ECO:0000269|PubMed:12834401}.
CC -!- DISRUPTION PHENOTYPE: Plants exhibit abscisic-acid-deficient phenotypes
CC in seeds, but not in vegetative tissues. {ECO:0000269|PubMed:11532178}.
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family. {ECO:0000305}.
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DR EMBL; AB028621; BAB01363.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76875.1; -; Genomic_DNA.
DR RefSeq; NP_189064.1; NM_113327.3.
DR AlphaFoldDB; Q9LRM7; -.
DR SMR; Q9LRM7; -.
DR STRING; 3702.AT3G24220.1; -.
DR MetOSite; Q9LRM7; -.
DR PaxDb; Q9LRM7; -.
DR PRIDE; Q9LRM7; -.
DR ProteomicsDB; 236816; -.
DR EnsemblPlants; AT3G24220.1; AT3G24220.1; AT3G24220.
DR GeneID; 822008; -.
DR Gramene; AT3G24220.1; AT3G24220.1; AT3G24220.
DR KEGG; ath:AT3G24220; -.
DR Araport; AT3G24220; -.
DR TAIR; locus:2093751; AT3G24220.
DR eggNOG; KOG1285; Eukaryota.
DR HOGENOM; CLU_016472_0_0_1; -.
DR InParanoid; Q9LRM7; -.
DR OMA; HSFHGTW; -.
DR OrthoDB; 524712at2759; -.
DR PhylomeDB; Q9LRM7; -.
DR BioCyc; MetaCyc:AT3G24220-MON; -.
DR PRO; PR:Q9LRM7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LRM7; baseline and differential.
DR Genevisible; Q9LRM7; AT.
DR GO; GO:0009570; C:chloroplast stroma; IDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:TAIR.
DR GO; GO:0045549; F:9-cis-epoxycarotenoid dioxygenase activity; IDA:TAIR.
DR GO; GO:0010436; F:carotenoid dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009688; P:abscisic acid biosynthetic process; TAS:TAIR.
DR GO; GO:0016121; P:carotene catabolic process; IBA:GO_Central.
DR GO; GO:0010114; P:response to red light; IEP:TAIR.
DR GO; GO:0009639; P:response to red or far red light; IEP:TAIR.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543; PTHR10543; 1.
DR Pfam; PF03055; RPE65; 1.
PE 2: Evidence at transcript level;
KW Abscisic acid biosynthesis; Chloroplast; Dioxygenase; Iron; Metal-binding;
KW Oxidoreductase; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN ?..577
FT /note="9-cis-epoxycarotenoid dioxygenase NCED6,
FT chloroplastic"
FT /id="PRO_0000285995"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 276
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 325
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 390
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 563
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
SQ SEQUENCE 577 AA; 63821 MW; 515E0A1B321B4FE1 CRC64;
MQHSLRSDLL PTKTSPRSHL LPQPKNANIS RRILINPFKI PTLPDLTSPV PSPVKLKPTY
PNLNLLQKLA ATMLDKIESS IVIPMEQNRP LPKPTDPAVQ LSGNFAPVNE CPVQNGLEVV
GQIPSCLKGV YIRNGANPMF PPLAGHHLFD GDGMIHAVSI GFDNQVSYSC RYTKTNRLVQ
ETALGRSVFP KPIGELHGHS GLARLALFTA RAGIGLVDGT RGMGVANAGV VFFNGRLLAM
SEDDLPYQVK IDGQGDLETI GRFGFDDQID SSVIAHPKVD ATTGDLHTLS YNVLKKPHLR
YLKFNTCGKK TRDVEITLPE PTMIHDFAIT ENFVVIPDQQ MVFKLSEMIR GGSPVIYVKE
KMARFGVLSK QDLTGSDINW VDVPDCFCFH LWNAWEERTE EGDPVIVVIG SCMSPPDTIF
SESGEPTRVE LSEIRLNMRT KESNRKVIVT GVNLEAGHIN RSYVGRKSQF VYIAIADPWP
KCSGIAKVDI QNGTVSEFNY GPSRFGGEPC FVPEGEGEED KGYVMGFVRD EEKDESEFVV
VDATDMKQVA AVRLPERVPY GFHGTFVSEN QLKEQVF
