NCED_ONCHC
ID NCED_ONCHC Reviewed; 610 AA.
AC C3VEQ3; Q52QS6;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 1.
DT 03-AUG-2022, entry version 27.
DE RecName: Full=9-cis-epoxycarotenoid dioxygenase, chloroplastic;
DE Short=OgNCED;
DE EC=1.13.11.51;
DE Flags: Precursor;
GN Name=NCED; Synonyms=NCED51;
OS Oncidium hybrid cultivar (Orchid).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC Epidendroideae; Cymbidieae; Oncidiinae; Oncidium.
OX NCBI_TaxID=141207;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=20635095; DOI=10.1007/s00425-010-1222-x;
RA Chiou C.Y., Pan H.A., Chuang Y.N., Yeh K.W.;
RT "Differential expression of carotenoid-related genes determines diversified
RT carotenoid coloration in floral tissues of Oncidium cultivars.";
RL Planta 232:937-948(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 182-427, AND TISSUE SPECIFICITY.
RX PubMed=16151849; DOI=10.1007/s00425-005-0113-z;
RA Hieber A.D., Mudalige-Jayawickrama R.G., Kuehnle A.R.;
RT "Color genes in the orchid Oncidium Gower Ramsey: identification,
RT expression, and potential genetic instability in an interspecific cross.";
RL Planta 223:521-531(2006).
CC -!- FUNCTION: Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity.
CC Catalyzes the first step of abscisic-acid biosynthesis from
CC carotenoids. Not active on the all-trans isomers of violaxanthin and
CC neoxanthin. Contributes probably to abscisic acid synthesis for the
CC induction of seed dormancy (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a
CC 12'-apo-carotenal; Xref=Rhea:RHEA:23328, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:51972, ChEBI:CHEBI:51973;
CC EC=1.13.11.51;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-
CC dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-xanthoxin;
CC Xref=Rhea:RHEA:16541, ChEBI:CHEBI:15379, ChEBI:CHEBI:32304,
CC ChEBI:CHEBI:34597, ChEBI:CHEBI:35305; EC=1.13.11.51;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-
CC didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-
CC xanthoxin; Xref=Rhea:RHEA:19677, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:34596, ChEBI:CHEBI:35306;
CC EC=1.13.11.51;
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000250|UniProtKB:O24592};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000250|UniProtKB:O24592};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in flower buds. Detected in sepals,
CC petals, lips and lip crests. {ECO:0000269|PubMed:16151849,
CC ECO:0000269|PubMed:20635095}.
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family. {ECO:0000305}.
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DR EMBL; FJ859994; ACP27628.1; -; mRNA.
DR EMBL; AY974326; AAX85470.1; -; mRNA.
DR AlphaFoldDB; C3VEQ3; -.
DR SMR; C3VEQ3; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0045549; F:9-cis-epoxycarotenoid dioxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009688; P:abscisic acid biosynthetic process; IEA:UniProtKB-KW.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543; PTHR10543; 1.
DR Pfam; PF03055; RPE65; 1.
PE 2: Evidence at transcript level;
KW Abscisic acid biosynthesis; Chloroplast; Dioxygenase; Iron; Metal-binding;
KW Oxidoreductase; Plastid; Stress response; Transit peptide.
FT TRANSIT 1..53
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 54..610
FT /note="9-cis-epoxycarotenoid dioxygenase, chloroplastic"
FT /id="PRO_0000426714"
FT BINDING 308
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 357
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT BINDING 422
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:O24592"
FT CONFLICT 426
FT /note="S -> A (in Ref. 2; AAX85470)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 610 AA; 67825 MW; 409AE6F73031F244 CRC64;
MASSMSLFIS SSSNQTVNSD TLRLKQSSKP TNLIHPNPIK LVRSRQVIAG SIQCSTTPNS
FGLDTTSPSP FYPLPPTCPK EIHPEQSAKP SRPSWNLFQR AAAAVLEAVE DNLIQNLLES
GHPLPKTADP AVQIAGNFAP VGEQKPHHDL PVDGRIPPLI NGVYLRNGAN PLFEPVAGHH
FFDGDGMVHA VHLRNGRASY ACRFTETERL KQERAVGRAI FPKAIGELHG HSGIARLLLF
YARGLLGLID HSRGTGVANA GLIYFNNRLL AMSEDDLPYH VRIKPNGDLE TAGRYDFDGQ
LTTTMIAHPK LDPETREFFA LSYDVIKKPY LKYFRFSPCG EKSPDVEIPL PQPTMMHDFA
ITKNFVIIPD QQVVFKLQEM ICGGSPVVYD KEKIARFGVL PKYAIDASEM QWIDVPDCFC
FHLWNSWEEP ETEEVVVIGS CMTPPDSIFN ESEENLQSVL TEIRLNLRTG KSTRRPILRP
GNSQINLEAG MVNRNRLGRR TRFAYLAIAE PWPKVSGFAK VDLASGEIQR FEYGDGGYGG
EPYFVPREGC DREDGGYVLA FVHDEREGSS ELLIMNAADM RLEAAVRLPS RVPYGFYGTF
VSATELHSQA
