NCER2_ARATH
ID NCER2_ARATH Reviewed; 757 AA.
AC Q304B9; F4IRY2; Q3EBK8; Q7XJQ9;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Neutral ceramidase 2 {ECO:0000303|PubMed:26150824};
DE Short=AtNCER2 {ECO:0000303|PubMed:26150824};
DE Short=N-CDase 2 {ECO:0000303|PubMed:26150824};
DE Short=NCDase 2 {ECO:0000303|PubMed:26150824};
DE EC=3.5.1.23 {ECO:0000250|UniProtKB:O06769};
DE AltName: Full=Acylsphingosine deacylase 2;
DE AltName: Full=N-acylsphingosine amidohydrolase 2;
DE Flags: Precursor;
GN Name=NCER2 {ECO:0000303|PubMed:26150824};
GN OrderedLocusNames=At2g38010 {ECO:0000312|Araport:AT2G38010};
GN ORFNames=T8P21.8 {ECO:0000305};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=26150824; DOI=10.3389/fpls.2015.00460;
RA Li J., Bi F.-C., Yin J., Wu J.-X., Rong C., Wu J.-L., Yao N.;
RT "An Arabidopsis neutral ceramidase mutant ncer1 accumulates
RT hydroxyceramides and is sensitive to oxidative stress.";
RL Front. Plant Sci. 6:460-460(2015).
CC -!- FUNCTION: Hydrolyzes the sphingolipid ceramide into sphingosine and
CC free fatty acid. {ECO:0000250|UniProtKB:F4HQM3}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an N-acylsphing-4-enine + H2O = a fatty acid + sphing-4-enine;
CC Xref=Rhea:RHEA:20856, ChEBI:CHEBI:15377, ChEBI:CHEBI:28868,
CC ChEBI:CHEBI:52639, ChEBI:CHEBI:57756; EC=3.5.1.23;
CC Evidence={ECO:0000250|UniProtKB:O06769};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9VA70}.
CC Endoplasmic reticulum {ECO:0000250|UniProtKB:F4HQM3}. Golgi apparatus
CC {ECO:0000250|UniProtKB:Q0JL46}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q304B9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q304B9-2; Sequence=VSP_058912;
CC -!- SIMILARITY: Belongs to the neutral ceramidase family. {ECO:0000305}.
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DR EMBL; CP002685; AEC09477.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09478.1; -; Genomic_DNA.
DR PIR; H84799; H84799.
DR RefSeq; NP_181337.2; NM_129358.3. [Q304B9-1]
DR RefSeq; NP_973628.1; NM_201899.2. [Q304B9-2]
DR AlphaFoldDB; Q304B9; -.
DR SMR; Q304B9; -.
DR BioGRID; 3723; 1.
DR STRING; 3702.AT2G38010.2; -.
DR MetOSite; Q304B9; -.
DR PaxDb; Q304B9; -.
DR PRIDE; Q304B9; -.
DR ProteomicsDB; 251251; -. [Q304B9-1]
DR EnsemblPlants; AT2G38010.1; AT2G38010.1; AT2G38010. [Q304B9-1]
DR EnsemblPlants; AT2G38010.2; AT2G38010.2; AT2G38010. [Q304B9-2]
DR GeneID; 818379; -.
DR Gramene; AT2G38010.1; AT2G38010.1; AT2G38010. [Q304B9-1]
DR Gramene; AT2G38010.2; AT2G38010.2; AT2G38010. [Q304B9-2]
DR KEGG; ath:AT2G38010; -.
DR Araport; AT2G38010; -.
DR TAIR; locus:2065685; AT2G38010.
DR eggNOG; KOG2232; Eukaryota.
DR HOGENOM; CLU_011300_2_0_1; -.
DR InParanoid; Q304B9; -.
DR OrthoDB; 967085at2759; -.
DR PhylomeDB; Q304B9; -.
DR BioCyc; ARA:AT2G38010-MON; -.
DR PRO; PR:Q304B9; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q304B9; baseline and differential.
DR Genevisible; Q304B9; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0102121; F:ceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0017040; F:N-acylsphingosine amidohydrolase activity; IBA:GO_Central.
DR GO; GO:0046514; P:ceramide catabolic process; IBA:GO_Central.
DR GO; GO:0042759; P:long-chain fatty acid biosynthetic process; IBA:GO_Central.
DR GO; GO:0046512; P:sphingosine biosynthetic process; IBA:GO_Central.
DR Gene3D; 2.60.40.2300; -; 1.
DR InterPro; IPR006823; Ceramidase_alk.
DR InterPro; IPR038445; NCDase_C_sf.
DR InterPro; IPR031331; NEUT/ALK_ceramidase_C.
DR InterPro; IPR031329; NEUT/ALK_ceramidase_N.
DR PANTHER; PTHR12670; PTHR12670; 1.
DR Pfam; PF04734; Ceramidase_alk; 1.
DR Pfam; PF17048; Ceramidse_alk_C; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Endoplasmic reticulum; Glycoprotein; Golgi apparatus;
KW Hydrolase; Lipid metabolism; Reference proteome; Secreted; Signal;
KW Sphingolipid metabolism.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..757
FT /note="Neutral ceramidase 2"
FT /id="PRO_0000247110"
FT ACT_SITE 330
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q9NR71"
FT CARBOHYD 311
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 348
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 657
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT VAR_SEQ 282
FT /note="D -> ESYPFLELPNVAILYIAVAERLMWKVMVLFYFVSES (in
FT isoform 2)"
FT /id="VSP_058912"
SQ SEQUENCE 757 AA; 83264 MW; 6586793BC42B1E42 CRC64;
MAVSLPLFQF ILFLLLLLLS RTVYAYLIGV GSYDITGPAA DVNMMGYANS DQIASGIHFR
LRARAFIVAE PQGNRVVFVN LDACMASQIV TIKVLERLKA RYGELYTEKN VAISGIHTHA
GPGGYLQYVT YIVTSLGFVR QSFDVVVNGI EQSIVQAHES LRPGSAFVNK GDLLDAGVNR
SPSSYLNNPA AERSKYKYDV DKEMTLVKFV DSQLGPTGSF NWFATHGTSM SRTNSLISGD
NKGAAARFME DWFENGQKNS VSSRNIPRRV STIVSDFSRN RDRLLDIAAT YKSSRGHSVD
KSLDVKTRVR NGSKRKFVSA FCQSNCGDVS PNTLGTFCID TGLPCDFNHS TCNGQNELCY
GRGPGYPDEF ESTRIIGEKQ FKMAVELFNK ATEKLQGKIG YQHAYLDFSN LDVTVPKAGG
GSETVKTCPA AMGFGFAAGT TDGPGAFDFK QGDDQGNVFW RLVRNVLRTP GPEQVQCQKP
KPILLDTGEM KEPYDWAPSI LPIQILRIGQ LVILSVPGEF TTMAGRRLRD AIKSFLISSD
PKEFSNNMHV VIAGLTNTYS QYIATFEEYE VQRYEGASTL YGRHTLTAYI QEFKKLATAL
VNGLTLPRGP QPPDLLDKQI SLLSPVVVDS TPLGVKFGDV KADVPPKSTF RRGQQVNATF
WSGCPRNDLM TEGSFAVVET LREGGKWAPV YDDDDFSLKF KWSRPAKLSS ESQATIEWRV
PESAVAGVYR IRHYGASKSL FGSISSFSGS SSAFVVV
