NCFA_DICDI
ID NCFA_DICDI Reviewed; 604 AA.
AC Q867T7; Q54IE2;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=NADPH oxidase activator;
DE Short=NOX activator;
DE AltName: Full=p67-phox-like factor;
GN Name=ncfA; ORFNames=DDB_G0288773;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=15950752; DOI=10.1016/j.bbamcr.2005.02.004;
RA Lardy B., Bof M., Aubry L., Paclet M.H., Morel F., Satre M., Klein G.;
RT "NADPH oxidase homologs are required for normal cell differentiation and
RT morphogenesis in Dictyostelium discoideum.";
RL Biochim. Biophys. Acta 1744:199-212(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: May function as an activator of NOX1, a superoxide-producing
CC NADPH oxidase. {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed during vegetative stage.
CC Expression decreases during development and is very low from 12 hours
CC (late aggregation stage). {ECO:0000269|PubMed:15950752}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:15950752}.
CC -!- MISCELLANEOUS: Although it only shares low sequence similarity with
CC members of the NCF2/NOXA1 family, it may ensure the same function as
CC NCF2/p67-phox.
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DR EMBL; AY221172; AAO62420.1; -; Genomic_DNA.
DR EMBL; AY224389; AAO72634.1; -; mRNA.
DR EMBL; AAFI02000125; EAL63008.1; -; Genomic_DNA.
DR RefSeq; XP_636535.1; XM_631443.1.
DR AlphaFoldDB; Q867T7; -.
DR SMR; Q867T7; -.
DR STRING; 44689.DDB0191152; -.
DR PaxDb; Q867T7; -.
DR ABCD; Q867T7; 6 sequenced antibodies.
DR EnsemblProtists; EAL63008; EAL63008; DDB_G0288773.
DR GeneID; 8626820; -.
DR KEGG; ddi:DDB_G0288773; -.
DR dictyBase; DDB_G0288773; ncfA.
DR eggNOG; KOG4225; Eukaryota.
DR HOGENOM; CLU_452313_0_0_1; -.
DR InParanoid; Q867T7; -.
DR OMA; HEYIDYK; -.
DR Reactome; R-DDI-5668599; RHO GTPases Activate NADPH Oxidases.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR Reactome; R-DDI-9013423; RAC3 GTPase cycle.
DR PRO; PR:Q867T7; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005829; C:cytosol; ISS:dictyBase.
DR GO; GO:0016176; F:superoxide-generating NADPH oxidase activator activity; ISS:dictyBase.
DR GO; GO:0006801; P:superoxide metabolic process; ISS:dictyBase.
DR CDD; cd00201; WW; 1.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR000270; PB1_dom.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR InterPro; IPR001202; WW_dom.
DR InterPro; IPR036020; WW_dom_sf.
DR SMART; SM00028; TPR; 2.
DR SUPFAM; SSF48452; SSF48452; 1.
DR SUPFAM; SSF51045; SSF51045; 1.
DR PROSITE; PS51745; PB1; 1.
DR PROSITE; PS50005; TPR; 2.
DR PROSITE; PS50293; TPR_REGION; 1.
DR PROSITE; PS50020; WW_DOMAIN_2; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..604
FT /note="NADPH oxidase activator"
FT /id="PRO_0000361531"
FT REPEAT 36..69
FT /note="TPR 1"
FT REPEAT 71..103
FT /note="TPR 2"
FT DOMAIN 309..384
FT /note="PB1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT DOMAIN 573..604
FT /note="WW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00224"
FT REGION 180..298
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 383..581
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 189..241
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..256
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 257..281
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 282..296
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 396..488
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 498..570
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 604 AA; 65254 MW; 503A267BA74F399E CRC64;
MLKQTIKKWN QSIERYESGN VGEALTILTS IEQSTSKINY NIGVMYIKSN NFRNAIEYFN
RSVEQDKYLA SSYYMRAIAH HMNGELNHAI VDYDETISKL RGHEYIDYKQ LGLDHKLLLA
EVLFNKALAL GRAGSSVALQ ATQCFSQPSD SQEFKNQCKK IQDGSQLNFS TRPIPLSLLF
KPPKVSDAPQ KQRSATTSSI QSSSPSTPMS SSPPSYILKG PSSPPSSSSP SSSSPSLSSS
SSPKLPPTPK PSFGSSPPPS SSSSSSSSSS SSSSSISPLT NKTLPPKPPP LPSKKLPSRP
ISCVIQDVKI TLKVFYKDRR LIQIPVPCNL STFIQKIELK FEITISDKFS LSFQLDGEEN
EINSQVQLDK MICMEINEIN VKDIIPSPSP SPSPSPDKTN NSTSSYSSSS SSSSSSSSSS
SSSSYDNKPK SSFIPKTTTR PILPPTTTTT TSTSNNFNRN ATLPKKFGST PSSPSFSSPS
SSSSGGGGGP PIPTRGSPSI SLLKQQNQTQ SINIPPKVPT SSRPKMTQSH SPPSSSPLSS
YSTSFQSVSS PSLSSSYNGS TSSYGGFSSS RPPPTPYPYQ VLYTDSNEKY YLNTETNETF
WELP
