ID   NCKP1_XENLA             Reviewed;        1128 AA.
AC   Q640K3;
DT   03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Nck-associated protein 1;
DE            Short=NAP 1;
GN   Name=nckap1;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION.
RX   PubMed=26434722; DOI=10.1534/genetics.115.183137;
RA   Sullivan-Brown J.L., Tandon P., Bird K.E., Dickinson D.J., Tintori S.C.,
RA   Heppert J.K., Meserve J.H., Trogden K.P., Orlowski S.K., Conlon F.L.,
RA   Goldstein B.;
RT   "Identifying Regulators of Morphogenesis Common to Vertebrate Neural Tube
RT   Closure and Caenorhabditis elegans Gastrulation.";
RL   Genetics 202:123-139(2016).
CC   -!- FUNCTION: Part of the WAVE complex that regulates lamellipodia
CC       formation. The WAVE complex regulates actin filament reorganization via
CC       its interaction with the Arp2/3 complex. Actin remodeling activity is
CC       regulated by RAC1 (By similarity). Plays a role in neural tube closure.
CC       {ECO:0000250, ECO:0000269|PubMed:26434722}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Cell projection,
CC       lamellipodium membrane {ECO:0000250}; Single-pass membrane protein
CC       {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Note=At the interface
CC       between the lamellipodial actin meshwork and the membrane.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HEM-1/HEM-2 family. {ECO:0000305}.
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DR   EMBL; BC082621; AAH82621.1; -; mRNA.
DR   RefSeq; NP_001087969.1; NM_001094500.1.
DR   AlphaFoldDB; Q640K3; -.
DR   SMR; Q640K3; -.
DR   DNASU; 494653; -.
DR   GeneID; 494653; -.
DR   KEGG; xla:494653; -.
DR   CTD; 494653; -.
DR   Xenbase; XB-GENE-949170; nckap1.L.
DR   OrthoDB; 138196at2759; -.
DR   Proteomes; UP000186698; Chromosome 9_10L.
DR   Bgee; 494653; Expressed in zone of skin and 19 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031258; C:lamellipodium membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0001843; P:neural tube closure; IMP:UniProtKB.
DR   InterPro; IPR019137; Nck-associated_protein-1.
DR   PANTHER; PTHR12093; PTHR12093; 1.
DR   Pfam; PF09735; Nckap1; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Cell projection; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..1128
FT                   /note="Nck-associated protein 1"
FT                   /id="PRO_0000364440"
FT   TRANSMEM        995..1015
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          640..665
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        647..665
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1128 AA;  128954 MW;  DA6549F35474E505 CRC64;
     MSRSVLQPSQ QKLAEKLTIL NDRGVGMLTR LYNIKKACGD PKAKPSYLID KNLESAVKFI
     VRKFPAVETR NNNQQLAQLQ KEKSEILKNL ALYYFTFVDV MEFKDHVCEL LNTIDVCQVF
     LDITVNFDLT KNYLDLIVTY TTLMIMLSRI EERKAIIGLY NYSHEMTHGA SDREYPRLGQ
     MIVDYENPLK KMMEEFVPHS KSLSDALISL QMVYPRRNLS AEQWRNAQLL SLISAPSTML
     NPAQSDTMPC EYLSLDTMEK WIILGFILCH GILNTDATSL NLWKLALQSS SCICLYRDEV
     LHFHKAAEDL FVNIRGYNKR INDIRECKEN AVSHAGATHR ERRKFLRSAL KELATVLSDQ
     PGLLGPKALF VFMALSFARD EIIWLLRHAD NIPKKIADDF MDKHIAELIF YMEELRAHVR
     KYGPVMQRYY VQYLSGFDAV VLNELVQNLS VCPEDESIIM SSFVNTMTSL SVKQVEDGEV
     FDFRGMRLDW FRLQAYTSVS KASLSLTDHR ELGKMMNTII FHTKMVDSLV EMLVETSDLS
     IFCFYSRAFE KMFQQCLELP SQSRYSISFP LLCTHFMSCT HELCPEERHH IGDRSLSLCN
     MFLDEMAKQA RNLITDICTE QCTLSDQLLP KHCAKTISQA VNKKSKKQTG KKGEPEREKP
     GVESMRKNRL VVTNLDKLHT ALSELCFSIN YAPNMVVWEH TFTPREYLTS HLEIRFTKSI
     VGMTMYNQVT QEIAKPSELL TSVRAYMTVL QSIENYVQID ITRVFNNVLL QQTQHLDSHG
     EPTITSLYTN WYLETLLRQV SNGHIAYFPA MKAFVNLPTE NELTFNAEEY SDISEMRALS
     ELLGPYGMKF LSESLMWHIS SQVAELKKLV VENVEVLTQM RTSFDKPEQM AAFFKRLTSV
     DSVLKRMTII GVILSFRSLA QEALKDVLSY HIPFLVSSVE DFKDHIPRET DMKVAMNVYE
     LSSAAGLPCE IDPALVVALS SQKSETISPE EEYKIACLLM VFVAVSLPTL ASNVMSQYSP
     AIEGHCNNIH CLAKAINQIA AALFTIHKGS IEDRLKEFLA LASSSLLKIG QETDKTTTRN
     RESVYLLLDM IVQESPFLTM DLLESCFPYV LLRNAYHAVY KQSVTSSS