NCKX1_RAT
ID NCKX1_RAT Reviewed; 1181 AA.
AC Q9QZM6; Q62932;
DT 13-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Sodium/potassium/calcium exchanger 1;
DE AltName: Full=Na(+)/K(+)/Ca(2+)-exchange protein 1;
DE AltName: Full=Retinal rod Na-Ca+K exchanger;
DE AltName: Full=Solute carrier family 24 member 1;
GN Name=Slc24a1; Synonyms=Nckx1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING, AND TISSUE SPECIFICITY.
RC STRAIN=Sprague-Dawley; TISSUE=Eye;
RX PubMed=10751314; DOI=10.1152/ajpcell.2000.278.4.c651;
RA Poon S., Leach S., Li X.-F., Tucker J.E., Schnetkamp P.P.M., Lytton J.;
RT "Alternatively spliced isoforms of the rat eye sodium/calcium+potassium
RT exchanger NCKX1.";
RL Am. J. Physiol. 278:C651-C660(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1067-1155.
RA White K.E., Gesek F.A., Friedman P.A.;
RL Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Calcium, potassium:sodium antiporter that transports 1 Ca(2+)
CC and 1 K(+) in exchange for 4 Na(+). Critical component of the visual
CC transduction cascade, controlling the calcium concentration of outer
CC segments during light and darkness. Light causes a rapid lowering of
CC cytosolic free calcium in the outer segment of both retinal rod and
CC cone photoreceptors and the light-induced lowering of calcium is caused
CC by extrusion via this protein which plays a key role in the process of
CC light adaptation. {ECO:0000250|UniProtKB:O60721}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in)
CC + 4 Na(+)(out); Xref=Rhea:RHEA:69967, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:29103, ChEBI:CHEBI:29108;
CC Evidence={ECO:0000250|UniProtKB:Q28139};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q28139};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q9QZM6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9QZM6-2; Sequence=VSP_006161;
CC Name=3;
CC IsoId=Q9QZM6-3; Sequence=VSP_006162;
CC Name=4;
CC IsoId=Q9QZM6-4; Sequence=VSP_006163;
CC -!- TISSUE SPECIFICITY: Highly expressed in the eye.
CC {ECO:0000269|PubMed:10751314}.
CC -!- PTM: The uncleaved signal sequence is required for efficient membrane
CC targeting and proper membrane integration and topology.
CC {ECO:0000250|UniProtKB:Q28139}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. SLC24A subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF176688; AAD53121.1; -; mRNA.
DR EMBL; U49235; AAB37753.1; -; mRNA.
DR RefSeq; NP_064475.1; NM_020090.1. [Q9QZM6-1]
DR RefSeq; XP_017451362.1; XM_017595873.1. [Q9QZM6-1]
DR AlphaFoldDB; Q9QZM6; -.
DR STRING; 10116.ENSRNOP00000051962; -.
DR GlyGen; Q9QZM6; 1 site.
DR PaxDb; Q9QZM6; -.
DR Ensembl; ENSRNOT00000077152; ENSRNOP00000074571; ENSRNOG00000052051. [Q9QZM6-1]
DR Ensembl; ENSRNOT00000100509; ENSRNOP00000087860; ENSRNOG00000052051. [Q9QZM6-3]
DR Ensembl; ENSRNOT00000101835; ENSRNOP00000091100; ENSRNOG00000052051. [Q9QZM6-2]
DR Ensembl; ENSRNOT00000116039; ENSRNOP00000087726; ENSRNOG00000052051. [Q9QZM6-4]
DR GeneID; 56814; -.
DR KEGG; rno:56814; -.
DR UCSC; RGD:620080; rat. [Q9QZM6-1]
DR CTD; 9187; -.
DR RGD; 620080; Slc24a1.
DR eggNOG; KOG1307; Eukaryota.
DR GeneTree; ENSGT01030000234532; -.
DR InParanoid; Q9QZM6; -.
DR OrthoDB; 1168500at2759; -.
DR PhylomeDB; Q9QZM6; -.
DR Reactome; R-RNO-425561; Sodium/Calcium exchangers.
DR PRO; PR:Q9QZM6; -.
DR Proteomes; UP000002494; Chromosome 8.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0031226; C:intrinsic component of plasma membrane; IDA:RGD.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0005262; F:calcium channel activity; IBA:GO_Central.
DR GO; GO:0008273; F:calcium, potassium:sodium antiporter activity; IDA:RGD.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IDA:RGD.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; ISO:RGD.
DR GO; GO:0060292; P:long-term synaptic depression; IBA:GO_Central.
DR GO; GO:0060291; P:long-term synaptic potentiation; IBA:GO_Central.
DR GO; GO:0071805; P:potassium ion transmembrane transport; ISO:RGD.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0035725; P:sodium ion transmembrane transport; ISO:RGD.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1420.30; -; 2.
DR InterPro; IPR004481; K/Na/Ca-exchanger.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR InterPro; IPR004817; SLC24A1.
DR PANTHER; PTHR10846; PTHR10846; 2.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00927; 2A1904; 1.
DR TIGRFAMs; TIGR00367; TIGR00367; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Antiport; Calcium; Calcium transport; Cell membrane;
KW Glycoprotein; Ion transport; Membrane; Phosphoprotein; Reference proteome;
KW Repeat; Sensory transduction; Signal; Symport; Transmembrane;
KW Transmembrane helix; Transport; Vision.
FT CHAIN 1..1181
FT /note="Sodium/potassium/calcium exchanger 1"
FT /id="PRO_0000223304"
FT SIGNAL 1..?
FT /note="Not cleaved"
FT /evidence="ECO:0000250|UniProtKB:Q28139"
FT TOPO_DOM 1..419
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 420..440
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 441..464
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 465..485
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 486..491
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 492..512
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 513..519
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 520..544
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 545..552
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 553..569
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 570..989
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 990..1010
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1011..1017
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1018..1038
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1039..1053
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1054..1074
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1075..1092
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1093..1113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1114..1121
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1122..1142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1143..1150
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1151..1171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1172..1181
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 461..501
FT /note="Alpha-1"
FT REPEAT 730..741
FT /note="1"
FT REPEAT 742..754
FT /note="2"
FT REPEAT 755..766
FT /note="3"
FT REPEAT 767..778
FT /note="4"
FT REPEAT 779..791
FT /note="5"
FT REPEAT 792..804
FT /note="6"
FT REPEAT 805..817
FT /note="7"
FT REPEAT 818..830
FT /note="8"
FT REPEAT 831..843
FT /note="9"
FT REPEAT 844..856
FT /note="10"
FT REPEAT 857..869
FT /note="11"
FT REPEAT 870..881
FT /note="12"
FT REPEAT 882..893
FT /note="13"
FT REPEAT 894..905
FT /note="14"
FT REPEAT 1061..1092
FT /note="Alpha-2"
FT REGION 107..232
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 255..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 300..323
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 598..617
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 650..983
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 730..905
FT /note="14 X approximate tandem repeats"
FT COMPBIAS 111..164
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 196..218
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 712..744
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 752..770
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 778..805
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 806..829
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 830..924
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 947..979
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 625
FT /note="Phosphoserine"
FT /evidence="ECO:0000255"
FT MOD_RES 690
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O60721"
FT CARBOHYD 271
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 598..710
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_006161"
FT VAR_SEQ 616..710
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_006162"
FT VAR_SEQ 652..679
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_006163"
SQ SEQUENCE 1181 AA; 129981 MW; B063C1C1193696AE CRC64;
MGKLIRMGTQ ERRLLRPKRL HWSRLLFFLG MLIIGSTYQH LRRPQNPPSM WTKVSSQQPI
KLAIRDLPND EITVTDKDPP EASSEVEGEM LAPQDTVIIG EAAPSIAMED TPNPPITTKI
TPTSLKNNYS PTTAGTRRQK ENIPPTPSRA PSHFISTSGR QRVKSYTPKP RGGRKSSSPT
HTREEGRMHT PSPAGAPRTI SPTSTEKDSE TTATYRMLEP RSERTAGKTS LKRMVPNTRT
FLTREVETDL VTSPSLVGKN TLGSPRRGER NSSTTPLRAV LQPTPATSEE QVTASIRMGS
TPATTEGSTA AQRIGNPLSG TSTPAVRIAS VTDREKRPST AHGTLVTPQV KAVLTTQVHR
CVVVKPAPAV PMTPSPSMTA ILFPEAPTSG PSALPPGWPN LHPKAEYPPD LFSVEDRRQG
WVVLHIFGMT YVFVALAIVC DEYFVPALGV ITDKLQISED VAGATFMAAG GSAPELFTSL
IGVFISHSNV GIGTIVGSAV FNILFVIGTC ALFSREILNL TWWPLFRDVS FYILDLSMLI
VFFLDSLIAW WESLLLLLAY ALYVFTMKWN KQIERWVKEQ LSRRPVAKVM ALGDLSKPSD
GAIEENEQQD NKKLKLPSVL TRGSSSASLH NSIIRSTIYH LMLHSLDPLG EARPSKDKQE
SLNQEARVLP QTKAESSSDE EEPAELPAVT VTPAPAPEDK GDQEEDPGCQ EDVDEAEHRG
DMTGEEGERE TEAEGKKDEE GETEAERKED GQEEETETKG KEKQEGETES EGKDEQEGET
EAEGKEADHE GETEAEGKEV EHEGETEAEG TEDEQEGETE AEGKEVEQEG ETEAEGKEVE
HEVETEAERK ETNHEGETEA EGKEADHEGE TEAEGNVEHQ GETEAEGKVE HEGETEAGEK
DEHEGQSETQ ADDTEVKDGE GEAEANAEDQ CETAQGEKGA DGGGGSDGGD SEEEEDEEDE
EEEEEEEEEE EEEESEEPLS LEWPESRQKQ AIYLFLLPIV FPLWLTIPDV RRQEARKFFV
ITFLGSIIWI AMFSYLMVWW AHQVGETIGI SEEIMGLTIL AAGTSIPDLI TSVIVARKGL
GDMAVSSSVG SNIFDITVGL PVPWLLFSLI NALQPIPVSS NGLFCAIVLL FLMLLFVIFS
IASCKWRMNK ILGFTMFLLY FVFLVISVML EDRIISCPVS V
