NCKX2_CHICK
ID NCKX2_CHICK Reviewed; 651 AA.
AC Q9IAL7; Q9IAL6;
DT 13-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Sodium/potassium/calcium exchanger 2;
DE AltName: Full=Na(+)/K(+)/Ca(2+)-exchange protein 2;
DE AltName: Full=Retinal cone Na-Ca+K exchanger;
DE AltName: Full=Solute carrier family 24 member 2;
GN Name=SLC24A2; Synonyms=NCKX2;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS IIA AND IIB), FUNCTION, TRANSPORTER
RP ACTIVITY, AND TISSUE SPECIFICITY.
RC TISSUE=Retina;
RX PubMed=10662833; DOI=10.1523/jneurosci.20-04-01424.2000;
RA Prinsen C.F.M., Szerencsei R.T., Schnetkamp P.P.M.;
RT "Molecular cloning and functional expression of the potassium-dependent
RT sodium-calcium exchanger from human and chicken retinal cone
RT photoreceptors.";
RL J. Neurosci. 20:1424-1434(2000).
CC -!- FUNCTION: Calcium, potassium:sodium antiporter that transports 1 Ca(2+)
CC and 1 K(+) in exchange for 4 Na(+) (PubMed:10662833). Required for
CC learming and memory by regulating neuronal Ca(2+), which is essential
CC for the development of synaptic plasticity (By similarity).
CC {ECO:0000250|UniProtKB:Q8BUN9, ECO:0000269|PubMed:10662833}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in)
CC + 4 Na(+)(out); Xref=Rhea:RHEA:69967, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:29103, ChEBI:CHEBI:29108;
CC Evidence={ECO:0000305|PubMed:10662833};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9UI40};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=IIA;
CC IsoId=Q9IAL7-1; Sequence=Displayed;
CC Name=IIB;
CC IsoId=Q9IAL7-2; Sequence=VSP_006166;
CC -!- TISSUE SPECIFICITY: Retinal cones (PubMed:10662833). Found in the cone
CC inner segment layer and in a subpopulation of ganglion cells
CC (PubMed:10662833). {ECO:0000269|PubMed:10662833}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. SLC24A subfamily. {ECO:0000305}.
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DR EMBL; AF177985; AAF25809.1; -; mRNA.
DR EMBL; AF177986; AAF25810.1; -; mRNA.
DR RefSeq; NP_001001772.1; NM_001001772.1. [Q9IAL7-2]
DR AlphaFoldDB; Q9IAL7; -.
DR SMR; Q9IAL7; -.
DR STRING; 9031.ENSGALP00000037460; -.
DR PaxDb; Q9IAL7; -.
DR GeneID; 414891; -.
DR KEGG; gga:414891; -.
DR CTD; 25769; -.
DR VEuPathDB; HostDB:geneid_414891; -.
DR eggNOG; KOG1307; Eukaryota.
DR InParanoid; Q9IAL7; -.
DR PhylomeDB; Q9IAL7; -.
DR PRO; PR:Q9IAL7; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0001917; C:photoreceptor inner segment; IDA:ARUK-UCL.
DR GO; GO:0005262; F:calcium channel activity; IBA:GO_Central.
DR GO; GO:0008273; F:calcium, potassium:sodium antiporter activity; IGI:ARUK-UCL.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0098703; P:calcium ion import across plasma membrane; IDA:ARUK-UCL.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR GO; GO:0060292; P:long-term synaptic depression; IBA:GO_Central.
DR GO; GO:0060291; P:long-term synaptic potentiation; IBA:GO_Central.
DR GO; GO:0007601; P:visual perception; IEA:InterPro.
DR Gene3D; 1.20.1420.30; -; 2.
DR InterPro; IPR004481; K/Na/Ca-exchanger.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR InterPro; IPR030236; SLC24A2.
DR PANTHER; PTHR10846; PTHR10846; 1.
DR PANTHER; PTHR10846:SF41; PTHR10846:SF41; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00367; TIGR00367; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Antiport; Calcium; Calcium transport; Cell membrane;
KW Glycoprotein; Ion transport; Membrane; Potassium; Potassium transport;
KW Reference proteome; Repeat; Sodium; Sodium transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..651
FT /note="Sodium/potassium/calcium exchanger 2"
FT /id="PRO_0000019369"
FT TOPO_DOM 1..38
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 60..134
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 156..179
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..206
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 228..245
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 267..459
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 460..480
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 481..487
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 488..508
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 509..523
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 524..544
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 545..562
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 563..583
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 584..592
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 593..613
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 614..620
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 621..641
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 642..651
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REPEAT 176..216
FT /note="Alpha-1"
FT REPEAT 531..562
FT /note="Alpha-2"
FT REGION 92..123
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 304..338
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 103..123
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 304..326
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 112
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 362..378
FT /note="Missing (in isoform IIB)"
FT /evidence="ECO:0000303|PubMed:10662833"
FT /id="VSP_006166"
SQ SEQUENCE 651 AA; 72696 MW; AE2701AA0EA67472 CRC64;
MALCKKTVGS VLEEWCLNEP LFGCKRHQNV RKKLRLIRII GLLVSVVAIS TFSLSISAFF
KMETHSTVLA SSLESQKLVH GHQRTLLDFM EQNEGSTPDS PTSMKHEAEH DNATEEHSKG
EYPEDLFSLE ERRKGAVILH VIGMIYMFIA LAIVCDEFFV PSLTVITEKL SISDDVAGAT
FMAAGGSAPE LFTSLIGVFI SHSNVGIGTI VGSAVFNILF VIGMCALFSR EILNLTWWPL
FRDVSFYIVD LILLIIFFLD NLIMWWESLT LLTAYFCYVT FMKFNVQVEE WVKKVLNRNK
VEKATTGDAE GKSPTAGDKD DQTLTTKPRL QRGGSSASLH NSLMRNSIFQ LMIHTLDPLA
EELGSYGNLK YYDTMTEEGK FKERASILHK IAKKKCQVED SERQNGAANH EKGAKVEVAV
TPPSDSGPVQ NGIAHNVDEE NEEDEDQPLS LAWPDTPRKQ LTYLLVLPIV FPLWVSLPDV
RNPRSRKFFP ITFFGSISWI AFFSYLMVWW AHQVGETIGI SEEIMGLTIL AAGTSIPDLI
TSVIVARKGL GDMAVSSSVG SNIFDITVGL PLPWLLYAVI NNFSPVTVSS NGLFCAIVLL
FIMLLFVILS IAFCKWRMNK FLGFLMFGLY FVFLIVSVLL EDKVIQCPVS I
