NCKX3_RAT
ID NCKX3_RAT Reviewed; 624 AA.
AC Q9EPQ0;
DT 13-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Sodium/potassium/calcium exchanger 3;
DE AltName: Full=Na(+)/K(+)/Ca(2+)-exchange protein 3;
DE AltName: Full=Solute carrier family 24 member 3;
DE Flags: Precursor; Fragment;
GN Name=Slc24a3; Synonyms=Nckx3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RX PubMed=11294880; DOI=10.1074/jbc.m102314200;
RA Kraev A., Quednau B.D., Leach S., Li X.-F., Dong H., Winkfein R.,
RA Perizzolo M., Cai X., Yang R., Philipson K.D., Lytton J.;
RT "Molecular cloning of a third member of the potassium-dependent sodium-
RT calcium exchanger gene family, NCKX3.";
RL J. Biol. Chem. 276:23161-23172(2001).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Calcium, potassium:sodium antiporter that transports 1 Ca(2+)
CC and 1 K(+) in exchange for 4 Na(+). {ECO:0000269|PubMed:11294880}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in)
CC + 4 Na(+)(out); Xref=Rhea:RHEA:69967, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:29103, ChEBI:CHEBI:29108;
CC Evidence={ECO:0000250|UniProtKB:Q9HC58};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9HC58};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Abundant in the brain. Expressed at low levels in
CC the aorta, uterus and intestine. {ECO:0000269|PubMed:11294880}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. SLC24A subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY009158; AAG32680.1; -; mRNA.
DR RefSeq; NP_445957.2; NM_053505.2.
DR AlphaFoldDB; Q9EPQ0; -.
DR SMR; Q9EPQ0; -.
DR STRING; 10116.ENSRNOP00000012683; -.
DR GlyGen; Q9EPQ0; 2 sites.
DR iPTMnet; Q9EPQ0; -.
DR PhosphoSitePlus; Q9EPQ0; -.
DR PaxDb; Q9EPQ0; -.
DR GeneID; 85267; -.
DR KEGG; rno:85267; -.
DR UCSC; RGD:69256; rat.
DR CTD; 57419; -.
DR RGD; 69256; Slc24a3.
DR eggNOG; KOG1307; Eukaryota.
DR InParanoid; Q9EPQ0; -.
DR OrthoDB; 1168500at2759; -.
DR PhylomeDB; Q9EPQ0; -.
DR Reactome; R-RNO-425561; Sodium/Calcium exchangers.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0071944; C:cell periphery; IDA:ARUK-UCL.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:RGD.
DR GO; GO:0005262; F:calcium channel activity; IBA:GO_Central.
DR GO; GO:0008273; F:calcium, potassium:sodium antiporter activity; IDA:RGD.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0030282; P:bone mineralization; ISO:RGD.
DR GO; GO:0070588; P:calcium ion transmembrane transport; ISO:RGD.
DR GO; GO:0006812; P:cation transport; IDA:RGD.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; ISO:RGD.
DR GO; GO:0010629; P:negative regulation of gene expression; ISO:RGD.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:RGD.
DR GO; GO:0071805; P:potassium ion transmembrane transport; ISO:RGD.
DR GO; GO:0035725; P:sodium ion transmembrane transport; ISO:RGD.
DR Gene3D; 1.20.1420.30; -; 2.
DR InterPro; IPR004481; K/Na/Ca-exchanger.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR InterPro; IPR030248; SLC24A3.
DR PANTHER; PTHR10846; PTHR10846; 1.
DR PANTHER; PTHR10846:SF22; PTHR10846:SF22; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00367; TIGR00367; 1.
PE 1: Evidence at protein level;
KW Antiport; Calcium; Calcium transport; Cell membrane; Glycoprotein;
KW Ion transport; Membrane; Phosphoprotein; Potassium; Potassium transport;
KW Reference proteome; Repeat; Signal; Sodium; Sodium transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL <1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..624
FT /note="Sodium/potassium/calcium exchanger 3"
FT /id="PRO_0000019372"
FT TOPO_DOM 26..88
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 110..133
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..163
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..191
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..212
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 213..215
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 237..465
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 466..486
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 487..491
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 492..512
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 513..530
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 531..551
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 552..561
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 562..582
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 583..596
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 597..617
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 618..624
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REPEAT 130..170
FT /note="Alpha-1"
FT REPEAT 499..530
FT /note="Alpha-2"
FT REGION 386..421
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 388..416
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 289
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT CARBOHYD 52
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 67
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT NON_TER 1
SQ SEQUENCE 624 AA; 69389 MW; ADCA175AF1BF1141 CRC64;
RDLLLSQLCF LASVALLLWS LSSLREQKEL DLMDLIGEDR KWMVGRKLMQ VNDTLTSEDA
GLRSSKNCTE PALHEFPRDI FSNEDRRQGA VVLHVLCAMY MFYALAIVCD DFFVPSLEKI
CERLHLSEDV AGATFMAAGS SAPELFTSVI GVFITKGDVG VGTIVGSAVF NILCIIGVCG
LFAGQVVALS SWCLLRDSIY YTLSVVALIV FIYDEKVSWW ESLVLVLMYL IYIIIMKYNA
CIHQCFERRT KGAGNMVNGL ANNAEIDDSS NCDATVVLLK KANFHRKASV IMVDELLSAY
PHQLSFSEAG LRIMITSHFP PKTRLSMASR MLINERQRLI NSRAYTNGES EVAIKIPIKH
TVENGTGPSS APDRGVNGTR RDDIVAEADN ETENENEDEN NENDEEEDED DDEGPYTPFD
PPSGKLETVK WAFTWPLSFV LYFTVPNCNK PHWEKWFMVT FASSTLWIAA FSYMMVWMVT
IIGYTLGIPD VIMGITFLAA GTSVPDCMAS LIVARQGMGD MAVSNSIGSN VFDILIGLGL
PWALQTLAVD YGSYIRLNSR GLIYSVGLLL ASVFVTVFGV HLNKWQLDKK LGCGCLFLYG
VFLCFSIMTE FNVFTFVNLP MCGD
