NCKX5_MOUSE
ID NCKX5_MOUSE Reviewed; 501 AA.
AC Q8C261; Q6XE39; Q8CJF1;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Sodium/potassium/calcium exchanger 5;
DE AltName: Full=Na(+)/K(+)/Ca(2+)-exchange protein 5;
DE AltName: Full=Solute carrier family 24 member 5;
DE Flags: Precursor;
GN Name=Slc24a5; Synonyms=Nckx5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ;
RA Satoh J., Smith C., Romero M.F.;
RT "Cloning and characterization of a novel, mouse renal divalent cation
RT transporter (JSX).";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Ishibashi K.;
RT "Cloning of a new member of Na-Ca exchanger.";
RL Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=16357253; DOI=10.1126/science.1116238;
RA Lamason R.L., Mohideen M.-A.P.K., Mest J.R., Wong A.C., Norton H.L.,
RA Aros M.C., Jurynec M.J., Mao X., Humphreville V.R., Humbert J.E., Sinha S.,
RA Moore J.L., Jagadeeswaran P., Zhao W., Ning G., Makalowska I.,
RA McKeigue P.M., O'Donnell D., Kittles R., Parra E.J., Mangini N.J.,
RA Grunwald D.J., Shriver M.D., Canfield V.A., Cheng K.C.;
RT "SLC24A5, a putative cation exchanger, affects pigmentation in zebrafish
RT and humans.";
RL Science 310:1782-1786(2005).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=22677781; DOI=10.1159/000337493;
RA Hu P., Lacruz R.S., Smith C.E., Smith S.M., Kurtz I., Paine M.L.;
RT "Expression of the sodium/calcium/potassium exchanger, NCKX4, in
RT ameloblasts.";
RL Cells Tissues Organs 196:501-509(2012).
CC -!- FUNCTION: Calcium, potassium:sodium antiporter that transports 1 Ca(2+)
CC and 1 K(+) to the melanosome in exchange for 4 cytoplasmic Na(+).
CC Involved in pigmentation, possibly by participating in ion transport in
CC melanosomes. Predominant sodium-calcium exchanger in melanocytes.
CC {ECO:0000250|UniProtKB:Q71RS6}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in)
CC + 4 Na(+)(out); Xref=Rhea:RHEA:69967, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:29103, ChEBI:CHEBI:29108;
CC Evidence={ECO:0000250|UniProtKB:Q71RS6};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000250|UniProtKB:Q71RS6}; Multi-pass membrane protein
CC {ECO:0000255}. Melanosome {ECO:0000250|UniProtKB:Q71RS6}.
CC -!- TISSUE SPECIFICITY: Highly expressed in melanin-producing cells such as
CC skin and eye compared to other tissues. Strongly overexpressed in
CC melanoma cell lines. Expressed in dental tissues.
CC {ECO:0000269|PubMed:16357253, ECO:0000269|PubMed:22677781}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. SLC24A subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC24122.1; Type=Frameshift; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=Skin-deep - Issue 74 of
CC September 2006;
CC URL="https://web.expasy.org/spotlight/back_issues/074";
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DR EMBL; AY236527; AAP57526.1; -; mRNA.
DR EMBL; AB085629; BAC24122.1; ALT_FRAME; mRNA.
DR EMBL; AK089225; BAC40800.1; -; mRNA.
DR EMBL; BC094232; AAH94232.1; -; mRNA.
DR CCDS; CCDS38226.1; -.
DR RefSeq; NP_778199.2; NM_175034.3.
DR AlphaFoldDB; Q8C261; -.
DR STRING; 10090.ENSMUSP00000063887; -.
DR iPTMnet; Q8C261; -.
DR PhosphoSitePlus; Q8C261; -.
DR MaxQB; Q8C261; -.
DR PaxDb; Q8C261; -.
DR PRIDE; Q8C261; -.
DR ProteomicsDB; 252929; -.
DR Antibodypedia; 24489; 98 antibodies from 28 providers.
DR DNASU; 317750; -.
DR Ensembl; ENSMUST00000070353; ENSMUSP00000063887; ENSMUSG00000035183.
DR GeneID; 317750; -.
DR KEGG; mmu:317750; -.
DR UCSC; uc008mbv.1; mouse.
DR CTD; 283652; -.
DR MGI; MGI:2677271; Slc24a5.
DR VEuPathDB; HostDB:ENSMUSG00000035183; -.
DR eggNOG; KOG1307; Eukaryota.
DR GeneTree; ENSGT01030000234532; -.
DR HOGENOM; CLU_007948_5_0_1; -.
DR InParanoid; Q8C261; -.
DR OMA; RLWAWIT; -.
DR OrthoDB; 1168500at2759; -.
DR PhylomeDB; Q8C261; -.
DR TreeFam; TF318759; -.
DR Reactome; R-MMU-425561; Sodium/Calcium exchangers.
DR BioGRID-ORCS; 317750; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Slc24a5; mouse.
DR PRO; PR:Q8C261; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8C261; protein.
DR Bgee; ENSMUSG00000035183; Expressed in blastocyst and 73 other tissues.
DR ExpressionAtlas; Q8C261; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005802; C:trans-Golgi network; ISO:MGI.
DR GO; GO:0005262; F:calcium channel activity; IBA:GO_Central.
DR GO; GO:0015085; F:calcium ion transmembrane transporter activity; IDA:ARUK-UCL.
DR GO; GO:0008273; F:calcium, potassium:sodium antiporter activity; ISO:MGI.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0070509; P:calcium ion import; IDA:ARUK-UCL.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR GO; GO:0034220; P:ion transmembrane transport; ISO:MGI.
DR GO; GO:0042438; P:melanin biosynthetic process; IMP:MGI.
DR GO; GO:0030318; P:melanocyte differentiation; IBA:GO_Central.
DR GO; GO:0048022; P:negative regulation of melanin biosynthetic process; IMP:MGI.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1420.30; -; 2.
DR InterPro; IPR004481; K/Na/Ca-exchanger.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR InterPro; IPR030245; SLC24A5.
DR PANTHER; PTHR10846; PTHR10846; 1.
DR PANTHER; PTHR10846:SF61; PTHR10846:SF61; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00367; TIGR00367; 1.
PE 2: Evidence at transcript level;
KW Antiport; Calcium; Calcium transport; Golgi apparatus; Ion transport;
KW Membrane; Potassium; Potassium transport; Reference proteome;
KW Sensory transduction; Signal; Sodium; Sodium transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..501
FT /note="Sodium/potassium/calcium exchanger 5"
FT /id="PRO_0000045754"
FT TOPO_DOM 30..66
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 88..111
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 133..136
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 158..170
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192..194
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 216..305
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..326
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 327..334
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..355
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 356..362
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 363..383
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 384..400
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 401..421
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 422..438
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 439..459
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 460..470
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 471..491
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 492..501
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 40
FT /note="T -> K (in Ref. 2; BAC24122)"
FT /evidence="ECO:0000305"
FT CONFLICT 97
FT /note="I -> V (in Ref. 1; AAP57526)"
FT /evidence="ECO:0000305"
FT CONFLICT 120
FT /note="A -> G (in Ref. 2; BAC24122)"
FT /evidence="ECO:0000305"
FT CONFLICT 342
FT /note="S -> C (in Ref. 2; BAC24122)"
FT /evidence="ECO:0000305"
FT CONFLICT 474
FT /note="V -> L (in Ref. 2; BAC24122)"
FT /evidence="ECO:0000305"
FT CONFLICT 488
FT /note="I -> V (in Ref. 2; BAC24122)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 501 AA; 54984 MW; B0AFD2BE7FC7AB34 CRC64;
MRTKWGPTWT RRVLLLGIFW VSAYLPVRGV SLPPRLPRAT GNSTQCAVSP ASEFPEGFFT
KQESTDGGIV IYFLIILYMC MAISIVCDKY FLPSLEIISD SLGLSQDVAG ATFMAAGSSA
PELVTAFLGV FITKGDIGIS TILGSAIYNL LGICAACGLL SNMVSTLSCW PLFRDCAVYA
VSVGAVFGII FDNRIYWYEG AGLLLIYGLY VLLLCFDTTI SRHVMKTCSP CCPCLARAME
ERIEQQTLLG WEDESQLFIR RQSRTDSGIF QEDSGYSQLS LSLHGLSQVS EDPPSVFSMP
EADLRRIFWV LSLPIITLLA LTTPDCRRKF WKNYFVITFF MSALWISAFT YILVWMVTVT
GETLGIPDTV MGLTLLAAGT SIPDTVTSVL VARKGKGDMA ISNIVGSNVF DMLCLGLPWF
IKTAFTNASA PIEVNSKGLT YITISLNISI LFLFLAVHFN GWKLDRKLGV VCLVLYLGLA
TLSVLYEIGI IGNNRIRGCG V
