NCKXH_DROME
ID NCKXH_DROME Reviewed; 633 AA.
AC Q9VN12; Q8IPM4; Q8SXA2;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 16-MAR-2016, sequence version 4.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Probable sodium/potassium/calcium exchanger CG1090;
DE AltName: Full=Na(+)/K(+)/Ca(2+)-exchange protein CG1090;
DE Flags: Precursor;
GN ORFNames=CG1090;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAN13306.4}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAN13306.4}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAM11079.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 159-568, AND RNA EDITING OF
RP POSITION 516.
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM11079.1};
RC TISSUE=Head {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP RNA EDITING OF POSITION 516.
RX PubMed=17018572; DOI=10.1261/rna.254306;
RA Stapleton M., Carlson J.W., Celniker S.E.;
RT "RNA editing in Drosophila melanogaster: new targets and functional
RT consequences.";
RL RNA 12:1922-1932(2006).
CC -!- FUNCTION: May function in the removal and maintenance of calcium
CC homeostasis. Transports one Ca(2+) and 1 K(+) in exchange for 4 Na(+)
CC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- RNA EDITING: Modified_positions=516 {ECO:0000269|PubMed:12537569,
CC ECO:0000269|PubMed:17018572}; Note=Partially edited. Target of Adar.
CC {ECO:0000269|PubMed:17018572};
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. SLC24A subfamily. {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM11079.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAF52139.3; -; Genomic_DNA.
DR EMBL; AE014297; AAN13306.4; -; Genomic_DNA.
DR EMBL; AY094726; AAM11079.1; ALT_SEQ; mRNA.
DR RefSeq; NP_001262252.1; NM_001275323.1.
DR RefSeq; NP_649459.4; NM_141202.4.
DR RefSeq; NP_730819.2; NM_164343.2.
DR AlphaFoldDB; Q9VN12; -.
DR BioGRID; 65771; 1.
DR STRING; 7227.FBpp0304720; -.
DR PaxDb; Q9VN12; -.
DR PRIDE; Q9VN12; -.
DR EnsemblMetazoa; FBtr0335201; FBpp0307188; FBgn0037238.
DR EnsemblMetazoa; FBtr0335202; FBpp0307189; FBgn0037238.
DR EnsemblMetazoa; FBtr0335203; FBpp0307190; FBgn0037238.
DR GeneID; 40551; -.
DR KEGG; dme:Dmel_CG1090; -.
DR UCSC; CG1090-RA; d. melanogaster.
DR FlyBase; FBgn0037238; CG1090.
DR VEuPathDB; VectorBase:FBgn0037238; -.
DR eggNOG; KOG1307; Eukaryota.
DR GeneTree; ENSGT01030000234532; -.
DR HOGENOM; CLU_007948_5_2_1; -.
DR InParanoid; Q9VN12; -.
DR OMA; ENMGYHA; -.
DR Reactome; R-DME-425561; Sodium/Calcium exchangers.
DR BioGRID-ORCS; 40551; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 40551; -.
DR PRO; PR:Q9VN12; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0037238; Expressed in brain and 6 other tissues.
DR ExpressionAtlas; Q9VN12; baseline and differential.
DR Genevisible; Q9VN12; DM.
DR GO; GO:0016021; C:integral component of membrane; ISS:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005262; F:calcium channel activity; IBA:GO_Central.
DR GO; GO:0008273; F:calcium, potassium:sodium antiporter activity; ISS:UniProtKB.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006816; P:calcium ion transport; ISS:UniProtKB.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR GO; GO:0006814; P:sodium ion transport; ISS:UniProtKB.
DR Gene3D; 1.20.1420.30; -; 2.
DR InterPro; IPR004481; K/Na/Ca-exchanger.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR PANTHER; PTHR10846; PTHR10846; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00367; TIGR00367; 1.
PE 2: Evidence at transcript level;
KW Antiport; Calcium; Calcium transport; Ion transport; Membrane; Potassium;
KW Potassium transport; Reference proteome; Repeat; RNA editing; Signal;
KW Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..633
FT /note="Probable sodium/potassium/calcium exchanger CG1090"
FT /evidence="ECO:0000255"
FT /id="PRO_0000280226"
FT TOPO_DOM 22..111
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 133..157
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 179..181
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 203..220
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 263..427
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 428..448
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 449..468
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 469..489
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 490..500
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 501..521
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 522..535
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 536..556
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 557..568
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 569..589
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 590..597
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 598..618
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 619..633
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT REPEAT 153..193
FT /note="Alpha-1"
FT /evidence="ECO:0000255"
FT REPEAT 506..537
FT /note="Alpha-2"
FT /evidence="ECO:0000255"
FT REGION 298..422
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 298..313
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 393..410
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 516
FT /note="S -> G (in RNA edited version)"
FT /evidence="ECO:0000269|PubMed:17018572"
SQ SEQUENCE 633 AA; 69570 MW; 0EF985C621304379 CRC64;
MWNMGLLFLI YYCVSIYSAK GDTKDGQVLP LDVGALAEDA GDAEANGEDT PERNSESSVL
DTTLAFEPQA STQSTPIHGA VPTWRPKRDN CTPPAIEQFP QPLMNKWARQ HGGLILHILV
AVFTFFGLAI VCDEYFVASL DRLCEELKLS PDVAGATFMA AGSSAPELAT VVIGVFFAKD
DIGISGVIGS AVFNIMFVIS VCALCSGTVC QLNWWPLVRD CFFYCVSILV MLIIIFNDVI
SCFESVVMLL CYVGYCVALH FNTELERWAL GLNLPFKLPS KEEQSALVTY KNVPEGSYTQ
ESVGQTQGQK ATDDSETRSA KPQSDYQDYS DPNPTWDPNA AWGDESQPNP VANPPPVDDW
GMGHSGQENM GYHADQPESV VTGDGPPAAV KSGGQVVSTQ ATSAGGNDYY KSTDKQREPR
RDPLLRPMEG GLPALVSWYV VYPIHFLCKK TMPDCRQEQY RNWYPFTFLM SMVWISFYSY
FMVWMITVIG STLAIPDTVM GLTFVAAGVS VPDALSSIAV IKEGFGDMAV SNAIGSNVFD
ILVCLGLPWF IQTAIIKPGS HVNVISKGLA YSTLSLFSTV VFLILSTHLN GWKLDKRLGI
ILMVWYLFFI TLASLYELNV FGYMNPPECP STY
