NCKX_DROME
ID NCKX_DROME Reviewed; 881 AA.
AC Q9U6A0; A4V0I2; C7LAF4; Q8IPE3; Q8SXD2; Q9VL95;
DT 13-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 4.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Sodium/potassium/calcium exchanger Nckx30C;
DE AltName: Full=Na(+)/K(+)/Ca(2+)-exchange protein Nckx30C;
GN Name=Nckx30C; ORFNames=CG18660;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND RNA EDITING OF POSITION 365.
RX PubMed=10545508; DOI=10.1083/jcb.147.3.659;
RA Haug-Collet K., Pearson B., Webel R., Szerencsei R.T., Winkfein R.J.,
RA Schnetkamp P.P.M., Colley N.J.;
RT "Cloning and characterization of a potassium-dependent sodium/calcium
RT exchanger in Drosophila.";
RL J. Cell Biol. 147:659-670(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-509 (ISOFORMS A/D), AND RNA
RP EDITING OF POSITION 365.
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 26-881 (ISOFORM F), AND RNA
RP EDITING OF POSITION 365.
RC STRAIN=Berkeley;
RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP RNA EDITING OF POSITION 365.
RX PubMed=17018572; DOI=10.1261/rna.254306;
RA Stapleton M., Carlson J.W., Celniker S.E.;
RT "RNA editing in Drosophila melanogaster: new targets and functional
RT consequences.";
RL RNA 12:1922-1932(2006).
CC -!- FUNCTION: May function in the removal and maintenance of calcium
CC homeostasis during signaling in the adult and in signaling events
CC during embryogenesis and patterning of imaginal disks. Transports one
CC Ca(2+) and 1 K(+) in exchange for 4 Na(+).
CC {ECO:0000269|PubMed:10545508}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=D;
CC IsoId=Q9U6A0-1; Sequence=Displayed;
CC Name=A; Synonyms=B;
CC IsoId=Q9U6A0-2; Sequence=VSP_023345;
CC Name=E;
CC IsoId=Q9U6A0-3; Sequence=VSP_043929, VSP_043930;
CC Name=F;
CC IsoId=Q9U6A0-4; Sequence=VSP_043929, VSP_043930, VSP_023345;
CC -!- TISSUE SPECIFICITY: Expressed in the adult nervous system. Expressed in
CC the photoreceptor cells as well as in the lamina, medulla, and optic
CC lobes of the brain. {ECO:0000269|PubMed:10545508}.
CC -!- DEVELOPMENTAL STAGE: Expressed during ventral nerve cord development in
CC the embryo and in larval imaginal disks. Expressed in a dorsal-ventral
CC pattern in the eye-antennal disk. {ECO:0000269|PubMed:10545508}.
CC -!- RNA EDITING: Modified_positions=365 {ECO:0000269|PubMed:10545508,
CC ECO:0000269|PubMed:12537569, ECO:0000269|PubMed:17018572,
CC ECO:0000269|Ref.5}; Note=Partially edited. Target of Adar.;
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. SLC24A subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM11027.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
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DR EMBL; AF190455; AAF07938.1; -; mRNA.
DR EMBL; AE014134; AAF52801.1; -; Genomic_DNA.
DR EMBL; AE014134; AAN10706.1; -; Genomic_DNA.
DR EMBL; AY094674; AAM11027.1; ALT_SEQ; mRNA.
DR EMBL; BT099704; ACV53068.1; -; mRNA.
DR RefSeq; NP_652011.3; NM_143754.5. [Q9U6A0-1]
DR RefSeq; NP_723482.1; NM_164869.3. [Q9U6A0-2]
DR RefSeq; NP_723483.1; NM_164870.3. [Q9U6A0-2]
DR AlphaFoldDB; Q9U6A0; -.
DR STRING; 7227.FBpp0292911; -.
DR GlyGen; Q9U6A0; 3 sites.
DR PaxDb; Q9U6A0; -.
DR PRIDE; Q9U6A0; -.
DR EnsemblMetazoa; FBtr0079926; FBpp0079516; FBgn0028704. [Q9U6A0-2]
DR EnsemblMetazoa; FBtr0079928; FBpp0079518; FBgn0028704. [Q9U6A0-2]
DR EnsemblMetazoa; FBtr0303908; FBpp0292911; FBgn0028704. [Q9U6A0-1]
DR EnsemblMetazoa; FBtr0303909; FBpp0292912; FBgn0028704. [Q9U6A0-3]
DR EnsemblMetazoa; FBtr0335506; FBpp0307474; FBgn0028704. [Q9U6A0-4]
DR GeneID; 45248; -.
DR KEGG; dme:Dmel_CG18660; -.
DR UCSC; CG18660-RB; d. melanogaster.
DR CTD; 45248; -.
DR FlyBase; FBgn0028704; Nckx30C.
DR VEuPathDB; VectorBase:FBgn0028704; -.
DR eggNOG; KOG1307; Eukaryota.
DR GeneTree; ENSGT01030000234532; -.
DR InParanoid; Q9U6A0; -.
DR OMA; TIPDCRY; -.
DR PhylomeDB; Q9U6A0; -.
DR Reactome; R-DME-425561; Sodium/Calcium exchangers.
DR BioGRID-ORCS; 45248; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Nckx30C; fly.
DR GenomeRNAi; 45248; -.
DR PRO; PR:Q9U6A0; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0028704; Expressed in brain and 18 other tissues.
DR ExpressionAtlas; Q9U6A0; baseline and differential.
DR Genevisible; Q9U6A0; DM.
DR GO; GO:0016021; C:integral component of membrane; IDA:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005262; F:calcium channel activity; IBA:GO_Central.
DR GO; GO:0008273; F:calcium, potassium:sodium antiporter activity; IDA:UniProtKB.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IDA:FlyBase.
DR GO; GO:0006816; P:calcium ion transport; IDA:FlyBase.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR GO; GO:0048749; P:compound eye development; IEP:FlyBase.
DR GO; GO:0006814; P:sodium ion transport; IMP:UniProtKB.
DR Gene3D; 1.20.1420.30; -; 2.
DR InterPro; IPR004481; K/Na/Ca-exchanger.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR030243; NCKX30C-like.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR PANTHER; PTHR10846; PTHR10846; 1.
DR PANTHER; PTHR10846:SF44; PTHR10846:SF44; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00367; TIGR00367; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Antiport; Calcium; Calcium transport; Glycoprotein;
KW Ion transport; Membrane; Potassium; Potassium transport;
KW Reference proteome; Repeat; RNA editing; Sodium; Sodium transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..881
FT /note="Sodium/potassium/calcium exchanger Nckx30C"
FT /id="PRO_0000209496"
FT TOPO_DOM 1..194
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 216..330
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 331..351
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 352..375
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 397..402
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 403..423
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 424..433
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 434..454
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 455..458
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 459..479
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 480..720
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 721..741
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 742..756
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 757..777
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 778..795
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 796..816
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 817..822
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 823..843
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 844..852
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 853..873
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 874..881
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 372..412
FT /note="Alpha-1"
FT REPEAT 764..795
FT /note="Alpha-2"
FT REGION 79..111
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 149..181
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 215..255
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 272..315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 508..565
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 79..93
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 224..247
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 282..315
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 69
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 514
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 551
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 497..509
FT /note="Missing (in isoform E and isoform F)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_043929"
FT VAR_SEQ 585
FT /note="D -> DDDVP (in isoform E and isoform F)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_043930"
FT VAR_SEQ 647..671
FT /note="Missing (in isoform A and isoform F)"
FT /evidence="ECO:0000303|PubMed:10545508, ECO:0000303|Ref.5"
FT /id="VSP_023345"
FT VARIANT 365
FT /note="K -> R (in RNA edited version)"
FT CONFLICT 110
FT /note="S -> F (in Ref. 1; AAF07938)"
FT /evidence="ECO:0000305"
FT CONFLICT 852
FT /note="K -> R (in Ref. 1; AAF07938 and 5; ACV53068)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 881 AA; 95129 MW; 8DA41EF395BD7653 CRC64;
MLQPTTCSKQ QQQRQQPAIA TAAVGGAAVQ ELLRKAAAKD HGALAAATAT ATAASTTATT
ATAATASRNS SNTWCHSDDM LSAGRSRSSS TTIDFNSRRR RGRLRGHAPS DLAMNKQHQA
TILDECVNIF KRLVLLTLKT ISATTITKAK TRSRTAAQLP ATSAASATSS RGASAEQLLH
PSSRSRCRSR RCLRLPIYSI LLLCLTTQGL GLGDAAKPRP AKQHFGGSNS NSPNQNQNHN
DVLGGVGAPS QTSGEDEAEI MYPFQSGEQM FGLEEDQEQD PELNSNAVPG SDEDNAGNQR
GINDTHNDNS TTTKTPLFPK DLFTKEQLEN GAVILHIIGV IYMFVALAIV CDEFFVPSLD
VIIEKLGITD DVAGATFMAA GGSAPELFTS VIGVFVSFDD VGIGTIVGSA VFNILFVIGM
CALFSKTVLS LTWWPLFRDC SFYSISLLVL IYFFRDNRIF WWEALILFTI YIGYVAFMKW
NVQVETCVKK MITKNKVTRV RSTDQLMPAG NAANSSETSM ATQPGGSVTS RAASETRSGP
PGSSNAGATG NSSGGGGTSG STQTGAKFRH GLLQLMIHTI DPLHDGKVDE KATQLHAIAS
LKVLLDATKP QRGGATTSAA NHVKINLKET TLADRPNGNI DTTLDSPSLS GRRPSWIEQR
VKIQTRKFSI KAPEIEDEPE PLSMAWPDTA RKRLTYVLVA PLLVPMWLTL PDTRTPRGKR
FFPVTFIGSI VWIAAFSYLM VWWANVAGDT ARIPPEVMGL TFLAAGTSIP DLITSVIVAR
KGFGDMAVSS SVGSNIFDVT VGLPIPWLLY GIIYGAPVEV NSVGMVCSIT ILFMMLVFVV
MSIACFRWRM NKGLGFTMFL LYFAFVAVSL MFEYDVITCP F
