ID   NCLN_CHICK              Reviewed;         562 AA.
AC   Q5ZJH2;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Nicalin;
DE   AltName: Full=Nicastrin-like protein;
DE   Flags: Precursor;
GN   Name=NCLN; ORFNames=RCJMB04_18d18;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=CB; TISSUE=Bursa of Fabricius;
RX   PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA   Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA   Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA   Hayashizaki Y., Buerstedde J.-M.;
RT   "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT   function analysis.";
RL   Genome Biol. 6:R6.1-R6.9(2005).
CC   -!- FUNCTION: Component of a ribosome-associated translocon complex
CC       involved in multi-pass membrane protein transport into the endoplasmic
CC       reticulum (ER) membrane and biogenesis (By similarity). May antagonize
CC       Nodal signaling and subsequent organization of axial structures during
CC       mesodermal patterning, via its interaction with NOMO (By similarity).
CC       {ECO:0000250|UniProtKB:Q6NZ07, ECO:0000250|UniProtKB:Q969V3}.
CC   -!- SUBUNIT: Forms a complex with NOMO and TMEM147, resulting in a
CC       stabilization of the 3 proteins, which are otherwise quickly degraded
CC       by the proteasome. The ribosome-associated ER translocon complex
CC       includes SEC61A1, SEC61B, SEC61G, TMCO1, CCDC47, NCLN/Nicalin, NOMO and
CC       TMEM147; in the absence of ribosomes, only the complex forms with
CC       NCLN/Nicalin, NOMO and TMEM147 remains intact. Participates in a large
CC       protein complex, which is not related to the gamma-secretase complex.
CC       {ECO:0000250|UniProtKB:Q969V3}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:Q969V3}; Single-pass membrane protein
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the nicastrin family. {ECO:0000305}.
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DR   EMBL; AJ720462; CAG32121.1; -; mRNA.
DR   AlphaFoldDB; Q5ZJH2; -.
DR   SMR; Q5ZJH2; -.
DR   STRING; 9031.ENSGALP00000021774; -.
DR   MEROPS; M28.978; -.
DR   VEuPathDB; HostDB:geneid_426048; -.
DR   eggNOG; KOG2526; Eukaryota.
DR   InParanoid; Q5ZJH2; -.
DR   PhylomeDB; Q5ZJH2; -.
DR   Proteomes; UP000000539; Unplaced.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0043022; F:ribosome binding; ISS:UniProtKB.
DR   GO; GO:0009966; P:regulation of signal transduction; IBA:GO_Central.
DR   InterPro; IPR016574; Nicalin.
DR   InterPro; IPR007484; Peptidase_M28.
DR   PANTHER; PTHR31826; PTHR31826; 2.
DR   Pfam; PF04389; Peptidase_M28; 1.
DR   PIRSF; PIRSF011018; Nicalin; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Glycoprotein; Membrane; Reference proteome; Signal;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..41
FT                   /evidence="ECO:0000255"
FT   CHAIN           42..562
FT                   /note="Nicalin"
FT                   /id="PRO_0000019690"
FT   TOPO_DOM        42..521
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        522..542
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        543..562
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          420..447
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        240
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   562 AA;  62423 MW;  F1EF2737679FADAE CRC64;
     MLEEAGEVLE SVLKASCLPL SFLLFVPAVL LLLGPPPAAE AAHESTVYRM QQYELGGQPY
     GTRSAVLNTE ARTVEADVLS RRCVMMRLVD FSYEQYQKAL RQSAGAVVII LPRSISSVPQ
     DVVKQFMEIE PEMLAMETIV PVYFAVEDDE LLSIYEQTRA ASASQGSASA AEVLLHTATA
     NGFQMVTSGA QSKAIHDWLI PSVEGRLTGL GGEDLPTVVI VAHYDSFGVA PWLSHGADSN
     GSGVSVLLEL ARLFSRLYTY RRTHAGYNLL FFASGGGKFN YQGTKRWLED NLDHTDSSLL
     QDNVAFVLCL DTLGRGNSLH LHVSKPPKEG TLQHAFLREL EMVVASQFPE VKFSMVHKKI
     NLAEDILAWE HERFAIRRLP AFTISHLESH RDSLRNSIMD RRSQVDTKAL TQEYQDHCGG
     FDEGHLQPNR EGSTCRSADL HGSDADPGGA ARISDGLAEQ SAQAAQLIDK DSTFLSTLEY
     YMGRYLKDVK QHHVKADKRD PEFVFYDQLK QVMNAYRVKP AIFDLLLAVC IAAYLGVAYV
     AVQNFGLLYR MIQRLSLKTK QQ