NCLN_DANRE
ID NCLN_DANRE Reviewed; 572 AA.
AC Q6NZ07; Q6PHK1;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Nicalin-1;
DE AltName: Full=Nicastrin-like protein 1;
DE Flags: Precursor;
GN Name=ncl1;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=15257293; DOI=10.1038/sj.emboj.7600307;
RA Haffner C., Frauli M., Topp S., Irmler M., Hofmann K., Regula J.T.,
RA Bally-Cuif L., Haass C.;
RT "Nicalin and its binding partner Nomo are novel Nodal signaling
RT antagonists.";
RL EMBO J. 23:3041-3050(2004).
CC -!- FUNCTION: Necessary for the biogenesis and transport of multi-pass
CC membrane proteins into the endoplasmic reticulum membrane (By
CC similarity). Antagonizes Nodal signaling and subsequent organization of
CC axial structures during mesodermal patterning. Ectopic expression
CC results in cyclopia, due to a defect in mesendoderm patterning
CC (PubMed:15257293). {ECO:0000250|UniProtKB:Q969V3,
CC ECO:0000269|PubMed:15257293}.
CC -!- SUBUNIT: Component of the ribosome-associated endoplasmic reticulum
CC translocon complex. {ECO:0000250|UniProtKB:Q969V3}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q969V3};
CC Single-pass type I membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6NZ07-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6NZ07-2; Sequence=VSP_013852;
CC -!- DEVELOPMENTAL STAGE: Strongly and ubiquitously expressed at early stage
CC of somatogenesis. Later, expression is prominent in the developing
CC retina, optic tectum and somites. {ECO:0000269|PubMed:15257293}.
CC -!- SIMILARITY: Belongs to the nicastrin family. {ECO:0000305}.
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DR EMBL; BC056523; AAH56523.2; -; mRNA.
DR EMBL; BC066393; AAH66393.1; -; mRNA.
DR RefSeq; NP_996940.1; NM_207057.1. [Q6NZ07-1]
DR RefSeq; XP_005161646.1; XM_005161589.3. [Q6NZ07-2]
DR AlphaFoldDB; Q6NZ07; -.
DR SMR; Q6NZ07; -.
DR STRING; 7955.ENSDARP00000076240; -.
DR PaxDb; Q6NZ07; -.
DR Ensembl; ENSDART00000081801; ENSDARP00000076240; ENSDARG00000058861. [Q6NZ07-1]
DR Ensembl; ENSDART00000132942; ENSDARP00000122266; ENSDARG00000058861. [Q6NZ07-2]
DR GeneID; 336844; -.
DR KEGG; dre:336844; -.
DR CTD; 56926; -.
DR ZFIN; ZDB-GENE-030131-8788; ncln.
DR eggNOG; KOG2526; Eukaryota.
DR GeneTree; ENSGT00500000044945; -.
DR HOGENOM; CLU_034102_2_0_1; -.
DR InParanoid; Q6NZ07; -.
DR OMA; RMHQYDL; -.
DR OrthoDB; 424149at2759; -.
DR PhylomeDB; Q6NZ07; -.
DR TreeFam; TF105849; -.
DR PRO; PR:Q6NZ07; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 22.
DR Bgee; ENSDARG00000058861; Expressed in testis and 27 other tissues.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:BHF-UCL.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043022; F:ribosome binding; ISS:UniProtKB.
DR GO; GO:0048484; P:enteric nervous system development; IMP:ZFIN.
DR GO; GO:1900176; P:negative regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry; IMP:BHF-UCL.
DR GO; GO:0009966; P:regulation of signal transduction; IDA:ZFIN.
DR InterPro; IPR016574; Nicalin.
DR InterPro; IPR007484; Peptidase_M28.
DR PANTHER; PTHR31826; PTHR31826; 1.
DR Pfam; PF04389; Peptidase_M28; 1.
DR PIRSF; PIRSF011018; Nicalin; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Developmental protein; Glycoprotein;
KW Membrane; Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..35
FT /evidence="ECO:0000255"
FT CHAIN 36..572
FT /note="Nicalin-1"
FT /id="PRO_0000019691"
FT TOPO_DOM 36..530
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 531..551
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 552..572
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 108
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 234
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 436
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 454
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_013852"
SQ SEQUENCE 572 AA; 63988 MW; 9D79E852161E83C3 CRC64;
MFEEAGEVLE NMLKVSFPLS LVLFLVLVCP LRAEAAHEFS VYRMQQYDLQ GQTYGSRNAI
LNTEARTVEA EVLSRRCVMM RLADFSYEKY QKALRQSAGA VVIILPHNMS TLPQDIVQQF
MELEPELLAT ETIVPVYFAL EDEELLSIYT QTQISSSSQG SSSAAEVLLH TATANGFQMV
TSGAQSKAVS DWAITSLEGR LTGSGGEDLP TIVLVAHYDS FGVAPWLSYG ADSNGSGVAI
LLELARLFSR LYSYKRTHAG YNLLFFLSGG GKFNYQGTKR WLEDNLDHTD ASLLQDNVAF
VLCLDTLGNS DNLHLHVSKP PKEGSPQYTL LKELETVVAH QHPDLKFAMV HKKINLADDT
LAWEHERFGI RRLPAFTLSH LESHRSPARH SIMDMRSVSP SLEGAGEATT GPHVDLGKLS
RNTKVIAETL ARVIYNLTEK GVTGDLEIFT EQMQVQEDQL ASLVDWLTAQ PRAAQLLDKD
SSIINTLEHQ LSRYLKDVKR HLVRADKRDP EFVFYDQLKQ TMNAYRVKPA IFDLLLAVCI
ASYLGVLYLA IQNFGLLYGF LRRVTAPRVK QH
