ID   NCMP_SACSY              Reviewed;         280 AA.
AC   A0A1X9WEP1; A0A5Q0HFI8;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2017, sequence version 1.
DT   03-AUG-2022, entry version 14.
DE   RecName: Full=Nocamycin O-methyltransferase {ECO:0000305};
DE            EC=2.1.1.351 {ECO:0000269|PubMed:28818448};
DE   AltName: Full=Carboxylate O-methyltransferase {ECO:0000303|PubMed:28599654};
GN   Name=ncmP {ECO:0000303|PubMed:28599654};
GN   ORFNames=EKG83_19125 {ECO:0000312|EMBL:QFZ24352.1};
OS   Saccharothrix syringae (Nocardiopsis syringae).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Saccharothrix.
OX   NCBI_TaxID=103733;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 51364 / DSM 43886 / JCM 6844 / KCTC 9398 / NBRC 14523 / NRRL
RC   B-16468 / INA 2240;
RX   PubMed=28599654; DOI=10.1186/s12934-017-0718-5;
RA   Mo X., Shi C., Gui C., Zhang Y., Ju J., Wang Q.;
RT   "Identification of nocamycin biosynthetic gene cluster from Saccharothrix
RT   syringae NRRL B-16468 and generation of new nocamycin derivatives by
RT   manipulating gene cluster.";
RL   Microb. Cell Fact. 16:100-100(2017).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51364 / DSM 43886 / JCM 6844 / KCTC 9398 / NBRC 14523 / NRRL
RC   B-16468 / INA 2240;
RX   PubMed=33136202; DOI=10.1007/s00284-020-02272-0;
RA   Mo X., Yang S.;
RT   "Complete genome of nocamycin-producing strain Saccharothrix syringae NRRL
RT   B-16468 reveals the biosynthetic potential for secondary metabolites.";
RL   Curr. Microbiol. 78:107-113(2021).
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, PATHWAY, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 51364 / DSM 43886 / JCM 6844 / KCTC 9398 / NBRC 14523 / NRRL
RC   B-16468 / INA 2240;
RX   PubMed=28818448; DOI=10.1016/j.bmcl.2017.08.010;
RA   Mo X., Gui C., Wang Q.;
RT   "Elucidation of a carboxylate O-methyltransferase NcmP in nocamycin
RT   biosynthetic pathway.";
RL   Bioorg. Med. Chem. Lett. 27:4431-4435(2017).
CC   -!- FUNCTION: Involved in the biosynthesis of nocamycin I and nocamycin II
CC       (PubMed:28818448). Catalyzes the methylation of nocamycin E to yield
CC       nocamycin I (PubMed:28818448). {ECO:0000269|PubMed:28818448}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=nocamycin E + S-adenosyl-L-methionine = nocamycin I + S-
CC         adenosyl-L-homocysteine; Xref=Rhea:RHEA:56844, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:141048, ChEBI:CHEBI:141049;
CC         EC=2.1.1.351; Evidence={ECO:0000269|PubMed:28818448};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:56845;
CC         Evidence={ECO:0000269|PubMed:28818448};
CC   -!- PATHWAY: Antibiotic biosynthesis. {ECO:0000269|PubMed:28818448}.
CC   -!- DISRUPTION PHENOTYPE: Mutant loses the capability to produce nocamycin
CC       I and nocamycin II. Mutant produces a new nocamycin intermediate, 21-
CC       demethyl-nocamycin I, which was designated as nocamycin E.
CC       {ECO:0000269|PubMed:28818448}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=QFZ24352.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; KY287782; ARS01468.1; -; Genomic_DNA.
DR   EMBL; CP034550; QFZ24352.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; A0A1X9WEP1; -.
DR   SMR; A0A1X9WEP1; -.
DR   KEGG; ag:ARS01468; -.
DR   KEGG; ssyi:EKG83_19125; -.
DR   BioCyc; MetaCyc:MON-20309; -.
DR   BRENDA; 2.1.1.351; 16293.
DR   Proteomes; UP000325787; Chromosome.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR041698; Methyltransf_25.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF13649; Methyltransf_25; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   1: Evidence at protein level;
KW   Antibiotic biosynthesis; Methyltransferase; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..280
FT                   /note="Nocamycin O-methyltransferase"
FT                   /id="PRO_0000452109"
SQ   SEQUENCE   280 AA;  29006 MW;  581A96A947709D59 CRC64;
     MVFDRLAGIY DATGVEFFRP VARRLLDLVD PRPGVDLLDV GCGRGAVLFP AAERVGPGGT
     VVGIDIAEPM VRATAAEAAE RGLGTVSVRL GDGADPAFPA GSFDVVTASM SAALFPDLPA
     VAARYARLLR PDGRIGLTGP VPPPSLREWA LGPLRVGAVV DAIAPEAVAA THPRIAALLG
     AHPFGAPGAV ADALRAAGFV EVRELHEDLE LRAPSAEALV GWTWSNGLRV YWELVEPDRR
     AAVAAELVRD LTAHAAGGPI TATYPVAYVT GRLRPAGGTP