NCOA2_XENLA
ID NCOA2_XENLA Reviewed; 1516 AA.
AC Q9W705;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Nuclear receptor coactivator 2;
DE Short=NCoA-2;
DE AltName: Full=Transcriptional intermediary factor 2;
DE Short=xTIF2;
GN Name=ncoa2; Synonyms=tif2;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=10704837; DOI=10.1016/s0925-4773(99)00280-4;
RA de la Calle-Mustienes E., Gomez-Skarmeta J.L.;
RT "XTIF2, a Xenopus homologue of the human transcription intermediary factor,
RT is required for a nuclear receptor pathway that also interacts with CBP to
RT suppress Brachyury and XMyoD.";
RL Mech. Dev. 91:119-129(2000).
CC -!- FUNCTION: Transcriptional coactivator for steroid receptors and nuclear
CC receptors. Coactivator of the steroid binding domain (AF-2) but not of
CC the modulating N-terminal domain (AF-1) (By similarity). Required in a
CC nuclear receptor pathway to suppress expression of dorsal mesoderm
CC genes. May play a role in the positive regulation of the circadian
CC clock (By similarity). {ECO:0000250|UniProtKB:Q15596,
CC ECO:0000250|UniProtKB:Q61026, ECO:0000269|PubMed:10704837}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q15596}.
CC -!- TISSUE SPECIFICITY: Expressed homogeneously during late blastula-early
CC gastrula stages, becoming highly expressed in the notochord in the late
CC gastrula and neurula stages. At hatching, restricted to a few areas
CC including the blood precursor region, prospective pronephros and
CC retina. {ECO:0000269|PubMed:10704837}.
CC -!- DOMAIN: Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL
CC motifs are essential for the association with nuclear receptors and, at
CC least in part, functionally redundant (By similarity).
CC {ECO:0000250|UniProtKB:Q15596}.
CC -!- DOMAIN: The LLXXLXXXL motif is involved in transcriptional coactivation
CC and CREBBP/CBP binding. {ECO:0000250|UniProtKB:Q15596}.
CC -!- DOMAIN: Contains 2 C-terminal transcription activation domains (AD1 and
CC AD2) that can function independently. {ECO:0000250|UniProtKB:Q15596}.
CC -!- SIMILARITY: Belongs to the SRC/p160 nuclear receptor coactivator
CC family. {ECO:0000305}.
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DR EMBL; AJ243119; CAB45389.1; -; mRNA.
DR RefSeq; NP_001081139.1; NM_001087670.1.
DR AlphaFoldDB; Q9W705; -.
DR SMR; Q9W705; -.
DR BioGRID; 99007; 1.
DR IntAct; Q9W705; 1.
DR PRIDE; Q9W705; -.
DR GeneID; 394405; -.
DR KEGG; xla:394405; -.
DR CTD; 394405; -.
DR Xenbase; XB-GENE-864856; ncoa2.S.
DR OrthoDB; 59971at2759; -.
DR Proteomes; UP000186698; Chromosome 6S.
DR Bgee; 394405; Expressed in spleen and 19 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0030374; F:nuclear receptor coactivator activity; ISS:UniProtKB.
DR GO; GO:0046966; F:nuclear thyroid hormone receptor binding; ISS:UniProtKB.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:UniProtKB.
DR GO; GO:0035556; P:intracellular signal transduction; IMP:UniProtKB.
DR GO; GO:0019216; P:regulation of lipid metabolic process; ISS:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0048511; P:rhythmic process; IEA:UniProtKB-KW.
DR CDD; cd00130; PAS; 1.
DR Gene3D; 4.10.280.10; -; 1.
DR Gene3D; 6.10.140.20; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR010011; NCO_DUF1518.
DR InterPro; IPR028822; NCOA2.
DR InterPro; IPR032565; NCOA2/3_DUF4927.
DR InterPro; IPR009110; Nuc_rcpt_coact.
DR InterPro; IPR014920; Nuc_rcpt_coact_Ncoa-typ.
DR InterPro; IPR037077; Nuc_rcpt_coact_Ncoa_int_sf.
DR InterPro; IPR017426; Nuclear_rcpt_coactivator.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR013767; PAS_fold.
DR InterPro; IPR014935; SRC/p160_LXXLL.
DR PANTHER; PTHR10684; PTHR10684; 2.
DR PANTHER; PTHR10684:SF2; PTHR10684:SF2; 2.
DR Pfam; PF07469; DUF1518; 2.
DR Pfam; PF16279; DUF4927; 1.
DR Pfam; PF08815; Nuc_rec_co-act; 1.
DR Pfam; PF00989; PAS; 1.
DR Pfam; PF08832; SRC-1; 1.
DR PIRSF; PIRSF038181; Nuclear_receptor_coactivator; 1.
DR SMART; SM01151; DUF1518; 2.
DR SMART; SM00353; HLH; 1.
DR SMART; SM00091; PAS; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR SUPFAM; SSF55785; SSF55785; 2.
DR SUPFAM; SSF69125; SSF69125; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS50112; PAS; 1.
PE 2: Evidence at transcript level;
KW Activator; Biological rhythms; Developmental protein; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..1516
FT /note="Nuclear receptor coactivator 2"
FT /id="PRO_0000094405"
FT DOMAIN 23..80
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 116..180
FT /note="PAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 386..406
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 457..518
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 532..601
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 700..727
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 775..795
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 955..979
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1284..1311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1324..1350
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 628..632
FT /note="LXXLL motif 1"
FT MOTIF 680..684
FT /note="LXXLL motif 2"
FT MOTIF 735..739
FT /note="LXXLL motif 3"
FT MOTIF 1064..1072
FT /note="LLXXLXXXL motif"
FT COMPBIAS 8..37
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 457..478
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 501..518
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 547..561
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 584..601
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 702..719
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 955..975
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1324..1339
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1516 AA; 166156 MW; 09851C00AB439A4A CRC64;
MGENLSDPSR AEPRKRKESL DQLGPSPKRS TEKRNREQEN KYIEELAELI FANFNDIDNL
NFKPDKCAIL KETVKQIRQI KEHEKTAAAN EDEVQKADVS STGQSVIDKD ALGPMMLEAL
DGFFFVVNRE GNVVFVSENV TQYLRYNQEE LMNTSVYSIL HVGDHSEFIK NLLPKSLVNG
VPRRNSHTFN CRMLVKPMME CEEERHDGQE THQKYESMQC FAVSQPKSIK EEGEDFQSCL
ICVARRVPVK ERPVPPPSES FTTRQDFQGK ITSLDTTSMR ALMRPGWEDM VRRCIQRFHS
QHDGEISYSR RHHQEVLRQG HATSPFYRFS LSDGTTVVAQ TKSRLMRSQT NNEPPLVLSL
HVLQREQNVC GLNQDLAGQA MGKTLNPVQS SSPAHQAMYG GNPGQDTTIS SNMNYAISGP
KEQMGLATGR FVGSGGMNHI SSLQATTPQG NNYALKMNSP SHGSPGMGQG QPNSMLSPRH
RVSPGVAGSP RIAPSPFSPA GSLHSPVSVC SSTGNSHSYT NSSLNALQAL SEGQGPLAPP
LSSPDLKGGN LQHSPGNMNP PQLRKMGSID SKESFGLYEE QPESATGQGE SGCHSNEQKD
CGENLSSVVD KTEGQSRLLD GKGQQKLLKL LTTKSDQMEP STLPSNTLGD MNKDSLSNFA
SNSMSASAHG TSLKEKHKIL HRLLQDSSSP VDLAKLTAEA TGKELSQESN STGPGSEVTI
KQEPVSPKKK EHALLRYLLD KDDTTDNVAD ITPKLERADN KVDPSSCPKL SAVKAEKEEP
NFGHTDQPGS DFDNLDEILD DLQNSQLSQL FSDTRHDGNS ADKQAIMNDL MQLAGENSTG
LPAWAQKQRM LRMQQNNGFN SQLAAQLGRL PNQNLPLDIH FQSQASAGSF AQMRSSGPYT
TVPQSGVINN QAMMGSQGNV RNSSPGIVGV NGPRPPLKPG DWGSQASAVR PACPTTSTAM
NRHDMSRSPT ASIPMRPGSQ VCPRQVLQSA VMNMGSSELD MNISGPQYTQ QQAPPNQTAP
WPNRILTIEQ PSFNNQNRQP FGSPADDLIC QPIVSESPAD DGNLLDQLYM ALRNFDGLEE
IDRALGIPEM VSQGQAVEQE SFGSPESNLM MEQKPPVYNH AYANQGQMAQ NSYQPMQDPG
FNPMGQRPSY GILRMQNRPG LRPTGMVQNQ PNQLRLQLQH RLQAQNRQQL MNPINNVSNM
NLAMRPGVPG QLREQGPINA QMLAQRQREI LSQHLRQKQL QQQQQQQQQQ QQQQQQQQQQ
QQQQQQQQQQ QQQHRAMMMR SQGLAMPPNM VGSGGIPASI NSPRIPQGST QQFPFPPNYG
TGIPSPPPFT SPFSPVPPSP GSQSLSHSSL HGSQMNLANQ GIMGSMGGQY GPVMNPQMQH
NAFQFANSGM SQQSDPGFTG ATTPQSPIMS PRMGHIQSSM MQQSQANPAY QSELNGWAQG
NPAGNSMFSQ QSPPHFGQQS GTSMYNSNNM NISVSMAANG NGMNNMNQMT GQINMTSVTS
VPTSGLSSMG PEQKYC
