NCOR1_CAEEL
ID NCOR1_CAEEL Reviewed; 1778 AA.
AC P34333; B5MBV9; P34332; Q8MYR1;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 19-FEB-2014, sequence version 4.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Nuclear receptor corepressor 1;
DE Short=N-CoR;
DE Short=N-CoR1;
DE AltName: Full=Gex-3-interacting protein 8;
DE AltName: Full=Prion-like-(Q/N-rich) domain-bearing protein 12;
GN Name=gei-8 {ECO:0000312|WormBase:C14B9.6a};
GN Synonyms=pqn-12 {ECO:0000312|WormBase:C14B9.6a};
GN ORFNames=C14B9.6 {ECO:0000312|WormBase:C14B9.6a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND C), FUNCTION, INTERACTION WITH
RP NHR-60, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=Bristol N2;
RX PubMed=23484030; DOI=10.1371/journal.pone.0058462;
RA Mikolas P., Kollarova J., Sebkova K., Saudek V., Yilma P., Kostrouchova M.,
RA Krause M.W., Kostrouch Z., Kostrouchova M.;
RT "GEI-8, a homologue of vertebrate nuclear receptor corepressor NCoR/SMRT,
RT regulates gonad development and neuronal functions in Caenorhabditis
RT elegans.";
RL PLoS ONE 8:E58462-E58462(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP INTERACTION WITH GEX-3.
RX PubMed=11922622; DOI=10.1006/bbrc.2002.6717;
RA Tsuboi D., Qadota H., Kasuya K., Amano M., Kaibuchi K.;
RT "Isolation of the interacting molecules with GEX-3 by a novel functional
RT screening.";
RL Biochem. Biophys. Res. Commun. 292:697-701(2002).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22078881; DOI=10.1016/j.cell.2011.10.017;
RA Yamamoto H., Williams E.G., Mouchiroud L., Canto C., Fan W., Downes M.,
RA Heligon C., Barish G.D., Desvergne B., Evans R.M., Schoonjans K.,
RA Auwerx J.;
RT "NCoR1 is a conserved physiological modulator of muscle mass and oxidative
RT function.";
RL Cell 147:827-839(2011).
CC -!- FUNCTION: Mediates transcriptional repression by certain nuclear
CC receptors. Plays a role in development and neuronal function. May play
CC a role in muscle-specific oxidative mitochondrial metabolism.
CC {ECO:0000269|PubMed:22078881, ECO:0000269|PubMed:23484030}.
CC -!- SUBUNIT: Interacts with gex-3. Interacts (via C-terminus) with nhr-60.
CC {ECO:0000269|PubMed:11922622, ECO:0000269|PubMed:23484030}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=P34333-2; Sequence=Displayed;
CC Name=c;
CC IsoId=P34333-3; Sequence=VSP_043602;
CC -!- TISSUE SPECIFICITY: In larvae, expressed in pharyngeal neurons, ventral
CC and dorsal nerve cords, tail neurons, egg-laying neurons and egg-laying
CC muscles. Detected in the neurons of the pharyngeal nerve ring, head
CC neurons, tail neurons and egg-laying muscles in adults. Detected in
CC male-specific tail ganglia and rays in males.
CC {ECO:0000269|PubMed:23484030}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout the developmental stages with
CC a 2-fold increase in late larval (L4) stage.
CC {ECO:0000269|PubMed:23484030}.
CC -!- DISRUPTION PHENOTYPE: Mutant animals display a progressive defect in
CC locomotion starting at the L2 stage, showing delayed response to
CC prodding, irregular thrashing movements and a low pharyngeal pumping
CC rate. Worms have shorter body length, convoluted intestine and develop
CC gonadogenesis defects including loss of spermathecae, sterility and
CC larval arrest at L4 stage. They also show abnormal cholinergic
CC signaling leading to early onset of paralysis in the presence of
CC aldicarb, an acetylcholinesterase inhibitor. RNAi-mediated knockdown
CC results in enlargement of mitochondria in the body wall muscle. Muscle-
CC specific knockdown shows an increased level of oxygen consumption.
CC {ECO:0000269|PubMed:22078881, ECO:0000269|PubMed:23484030}.
CC -!- SIMILARITY: Belongs to the N-CoR nuclear receptor corepressors family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FO080531; CCD64442.1; -; Genomic_DNA.
DR EMBL; FO080531; CCD64444.2; -; Genomic_DNA.
DR PIR; S44758; S44758.
DR PIR; S44759; S44759.
DR RefSeq; NP_498773.3; NM_066372.4. [P34333-2]
DR RefSeq; NP_741245.3; NM_171207.5. [P34333-3]
DR AlphaFoldDB; P34333; -.
DR SMR; P34333; -.
DR BioGRID; 41351; 3.
DR DIP; DIP-24789N; -.
DR IntAct; P34333; 1.
DR STRING; 6239.C14B9.6a; -.
DR EPD; P34333; -.
DR PaxDb; P34333; -.
DR PeptideAtlas; P34333; -.
DR EnsemblMetazoa; C14B9.6a.1; C14B9.6a.1; WBGene00001565. [P34333-2]
DR EnsemblMetazoa; C14B9.6c.1; C14B9.6c.1; WBGene00001565. [P34333-3]
DR GeneID; 176145; -.
DR KEGG; cel:CELE_C14B9.6; -.
DR UCSC; C14B9.6c; c. elegans. [P34333-2]
DR CTD; 176145; -.
DR WormBase; C14B9.6a; CE42991; WBGene00001565; gei-8. [P34333-2]
DR WormBase; C14B9.6c; CE48463; WBGene00001565; gei-8. [P34333-3]
DR eggNOG; KOG1878; Eukaryota.
DR GeneTree; ENSGT00940000175552; -.
DR InParanoid; P34333; -.
DR OMA; NECKMQY; -.
DR OrthoDB; 544239at2759; -.
DR PRO; PR:P34333; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00001565; Expressed in embryo and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00167; SANT; 1.
DR InterPro; IPR001005; SANT/Myb.
DR InterPro; IPR017884; SANT_dom.
DR SMART; SM00717; SANT; 2.
DR PROSITE; PS50090; MYB_LIKE; 1.
DR PROSITE; PS51293; SANT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..1778
FT /note="Nuclear receptor corepressor 1"
FT /id="PRO_0000197148"
FT DOMAIN 125..176
FT /note="SANT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00624"
FT DOMAIN 356..398
FT /note="Myb-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00133"
FT REGION 409..671
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 992..1024
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1195..1218
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1276..1339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1414..1434
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1646..1718
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 422..437
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 493..517
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 521..548
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 574..593
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 597..612
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 621..636
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 647..671
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1317..1339
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1171..1253
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000303|PubMed:23484030"
FT /id="VSP_043602"
SQ SEQUENCE 1778 AA; 197922 MW; 4042B7F3E6A15999 CRC64;
MESFKELARE YDEKFKAFQD DLQKWEETSE RKEYAEFHRV QAESEFPELK REREDRERWA
RAERIRGEDE KSMLAKEHAD KKIRLGVAKI PRLLTESERK MDEFVERPGS ILKDMKKEHR
QSVLDRLEEW SPEERSLFKS RQADHVKIFH GLTEFFVDKT ASDLVLFYYM NKKTEDYKKD
FKPKKRVTKY KVGAFPSVEE LAYFRMMPPL DFSSFPKNSL MCYFCCRTVN GIDLNGTFMP
KEAYEIFAIC PDEDRVVCSG CREEAAKLYK DNRCFGNRCS NQKKRANRVN RNIPLDFADF
PVRTRAFIMD KLGSTRVAVK FCTPCKNALT RWINDVNNKE ETIMAELLNY EGQVGWTDDE
KTKLVTLINS SPTLDWVSIS EGMNRRPNEC KMQYDAMNGV KTQPMIEEVD EEDGNGQEEG
GDALVNTPTT SSAAARRSGL ARNAKKPVRT PRAPRSAGGR RTGGAVTRAQ AVPKPVEDLG
EEIDEMEIED NDEDASRGSR GKDSKAPSDR DGSPADMEGD SPEGQDQDAD QDQDQDQDVD
EEEEEVIVRD IDSPVKTLLS PKILSGGHKP DFPPVPRIQK PSTSSQPPPP EPMDTKENES
DDGEEDNDIL EIDVDEPPAK RPTPTSSSSH LIGSSSVGGS ERELGGRGLV QQQQQQQPQA
QSAAPPVTVS TAAAATAERL VNATSPSPSV ASQHLVPTET STSVPPVTIV PPAIQQPVVV
ISGAAPQFTQ QQTTHSPALI AQPIPQQLIP QRVSTPAQIL TPTPVRPTAA STPSMDQFLG
LFKQQQQQQQ QQPQQSNLMQ QLGNINPQFL ALLLQQQQQQ QQVQQAQVQA AQTQGSLTSG
TPFQAQQRPD EALQKLFSSP EMLGTLLNAK YQFPQFPQAI QQNPLLMNAQ HQLILQQQQL
AMQHAQAQAA QAAQAQAAQA AQAQAQAQAE AKLKTQAAQA KAQADAQARV QTQQQAHLKA
QAQAQYQMNR PQLIPASVQM PIGINTAYHQ KSLTPSETSA SATPPAHRPR AATTVGSKMP
AGRSNVQEAE LRTLKEELIK RIRLFRDRIA EDAHLKREEE NIVTYTAQIQ ASRVQYNTEI
LGQMKQRYEQ AAARRIEIVK ELDEPAKFIN GVQNKFNDFS VFKLDEIDRQ TLHEILQRYA
AEQKPDQLQQ QQNDFHNLLR QNNIGIRQAQ YPALQASPHQ AAIIQHQQQQ EAFKKLQQQH
QDAQKRKLEA PVSSATPQVK RIALSQSSPV QRFSQHPNGT LPHNLAVVYQ NKMPQDREKL
LQEQQLNQYL FAAQQQHLQQ QQQHHGTQPE QKSKRKSGIE SITSMQGAPH RHIQLAGPSS
SRIQSGRGVS PALSASRSVA PTISGAAGRS ITAGTSGHSS TSDYNKELAE RMNEVFKPTH
PQLLQTKASA MNSNAVSPAN TNNSDEIECV YQGPPKTPAS IKRLQPTEGP RTLSGQRMKS
ILSVPAHQRR SSIPPVKTED EAMECLSMMM YEEAKAPPSD RIVTKLISSS EAAAAANPSA
KFSYLDIFND VVKRDEERCQ RMLQSTSVLQ PSRELDAFLQ QLQQNPQQYA NLSAAEKLAL
QQYQIHSAHQ KSQQQAQLQA AQQLQQQQQA RPDHYEKFHL LRPNAEIVRP IPTTFSHFLN
KATTSTVSSS ASAPQFLQPP AAASIAAPTP ISTPHAMPSP SVGVQTAPPT PQAVHHPVMP
IIPGKPSSVN SNVSDVSSDD DDVRNPEKLP ANRLPPLPGD KTAFRSVIDL RATGHVAVTK
KIPVKYPLVV QAQNGIQPIK DYGPPCKNLV YEDLSDDE
