NCS1_CAEEL
ID NCS1_CAEEL Reviewed; 191 AA.
AC P36608; Q18608;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Neuronal calcium sensor 1;
DE Short=NCS-1;
GN Name=ncs-1; ORFNames=C44C1.3;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Bristol N2;
RX PubMed=7488079; DOI=10.1006/bbrc.1995.2601;
RA de Castro E., Nef S., Fiumelli H., Lenz S.E., Kawamura S., Nef P.;
RT "Regulation of rhodopsin phosphorylation by a family of neuronal calcium
RT sensors.";
RL Biochem. Biophys. Res. Commun. 216:133-140(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION.
RX PubMed=11343658; DOI=10.1016/s0896-6273(01)00276-8;
RA Gomez M., de Castro E., Guarin E., Sasakura H., Kuhara A., Mori I.,
RA Bartfai T., Bargmann C.I., Nef P.;
RT "Calcium-signalling via the neuron calcium sensor-1 regulates associative
RT learning and memory in C.elegans.";
RL Neuron 30:241-248(2001).
CC -!- FUNCTION: Neuronal calcium sensor, regulator of G protein-coupled
CC receptor phosphorylation in a calcium dependent manner. Can substitute
CC for calmodulin and directly activate PDE, NO synthase, and calcineurin.
CC Regulates associative learning and memory in a calcium dependent
CC manner. {ECO:0000269|PubMed:11343658}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:P62166,
CC ECO:0000250|UniProtKB:P62168}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P62166}.
CC -!- MISCELLANEOUS: Binds three calcium ions. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the recoverin family. {ECO:0000305}.
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DR EMBL; L33680; AAA85631.1; -; mRNA.
DR EMBL; FO080474; CCD63971.1; -; Genomic_DNA.
DR PIR; T29566; T29566.
DR RefSeq; NP_508186.1; NM_075785.1.
DR AlphaFoldDB; P36608; -.
DR SMR; P36608; -.
DR STRING; 6239.C44C1.3; -.
DR EPD; P36608; -.
DR PaxDb; P36608; -.
DR PeptideAtlas; P36608; -.
DR EnsemblMetazoa; C44C1.3.1; C44C1.3.1; WBGene00003563.
DR GeneID; 180448; -.
DR KEGG; cel:CELE_C44C1.3; -.
DR UCSC; C44C1.3; c. elegans.
DR CTD; 180448; -.
DR WormBase; C44C1.3; CE24845; WBGene00003563; ncs-1.
DR eggNOG; KOG0044; Eukaryota.
DR GeneTree; ENSGT00940000163010; -.
DR HOGENOM; CLU_072366_1_2_1; -.
DR InParanoid; P36608; -.
DR OMA; EYVFNVF; -.
DR OrthoDB; 1369072at2759; -.
DR PhylomeDB; P36608; -.
DR PRO; PR:P36608; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00003563; Expressed in larva and 3 other tissues.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043025; C:neuronal cell body; IDA:WormBase.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0008048; F:calcium sensitive guanylate cyclase activator activity; IBA:GO_Central.
DR GO; GO:0007635; P:chemosensory behavior; IMP:UniProtKB.
DR GO; GO:0006935; P:chemotaxis; IMP:UniProtKB.
DR GO; GO:0010446; P:response to alkaline pH; IMP:UniProtKB.
DR CDD; cd00051; EFh; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR028846; Recoverin.
DR PANTHER; PTHR23055; PTHR23055; 1.
DR Pfam; PF13202; EF-hand_5; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 2: Evidence at transcript level;
KW Calcium; Lipoprotein; Membrane; Metal-binding; Myristate;
KW Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..191
FT /note="Neuronal calcium sensor 1"
FT /id="PRO_0000073793"
FT DOMAIN 24..59
FT /note="EF-hand 1"
FT /evidence="ECO:0000305"
FT DOMAIN 60..95
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 96..131
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 144..179
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 73
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 75
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 77
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 79
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 84
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 109
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 111
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 113
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 115
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 120
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 157
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 159
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 161
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 163
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 168
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 191 AA; 22022 MW; 66D2A71BAAC2C044 CRC64;
MGKGNSKLKS SQIRDLAEQT YFTEKEIKQW YKGFVRDCPN GMLTEAGFQK IYKQFFPQGD
PSDFASFVFK VFDENKDGAI EFHEFIRALS ITSRGNLDEK LHWAFKLYDL DQDGFITRNE
MLSIVDSIYK MVGSSVQLPE EENTPEKRVD RIFRMMDKNN DAQLTLEEFK EGAKADPSIV
HALSLYEGLS S
