ID   NCTA_ASPFC              Reviewed;         247 AA.
AC   B0XTT5;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=NCT transcriptional regulatory complex subunit A {ECO:0000303|PubMed:31969561};
DE   AltName: Full=Negative cofactor 2 AB {ECO:0000303|PubMed:31969561};
GN   Name=nctA {ECO:0000303|PubMed:31969561}; ORFNames=AFUB_029870;
OS   Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus
OS   fumigatus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=451804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CEA10 / CBS 144.89 / FGSC A1163;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA   Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA   Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA   Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA   Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA   White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA   Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH NCTB AND MOT1, SUBCELLULAR
RP   LOCATION, AND DNA-BINDING.
RX   PubMed=31969561; DOI=10.1038/s41467-019-14191-1;
RA   Furukawa T., van Rhijn N., Fraczek M., Gsaller F., Davies E., Carr P.,
RA   Gago S., Fortune-Grant R., Rahman S., Gilsenan J.M., Houlder E.,
RA   Kowalski C.H., Raj S., Paul S., Cook P., Parker J.E., Kelly S.,
RA   Cramer R.A., Latge J.P., Moye-Rowley S., Bignell E., Bowyer P.,
RA   Bromley M.J.;
RT   "The negative cofactor 2 complex is a key regulator of drug resistance in
RT   Aspergillus fumigatus.";
RL   Nat. Commun. 11:427-427(2020).
CC   -!- FUNCTION: Part of the NCT transcriptional regulatory complex that acts
CC       as a key regulator of ergosterol biosynthesis and the azole exporter
CC       cdr1B (PubMed:31969561). The NCT complex binds the promoters of genes
CC       linked to azole susceptibility, and especially represses the expression
CC       of cdr1B transporter (PubMed:31969561). {ECO:0000269|PubMed:31969561}.
CC   -!- SUBUNIT: Forms the NCT transcriptional regulatory complex with nctB and
CC       mot1. {ECO:0000269|PubMed:31969561}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:31969561}.
CC   -!- DISRUPTION PHENOTYPE: Results in a reduction in growth rate
CC       (PubMed:31969561). Leads to a multidrug-resistance phenotype, including
CC       the azoles (itraconazole, voriconazole and posaconazole), as well as
CC       the salvage therapeutic amphotericin B and terbinafine, an agent used
CC       in the treatment of chronic and allergic disease (PubMed:31969561).
CC       Leads also to transcriptional derepression of cdr1B and its over-
CC       production (PubMed:31969561). Results also in a notable increase in the
CC       immunogenic properties of Aspergillus fumigatus, but does not result in
CC       loss of virulence (PubMed:31969561). {ECO:0000269|PubMed:31969561}.
CC   -!- SIMILARITY: Belongs to the NC2 alpha/DRAP1 family. {ECO:0000305}.
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DR   EMBL; DS499595; EDP54927.1; -; Genomic_DNA.
DR   AlphaFoldDB; B0XTT5; -.
DR   SMR; B0XTT5; -.
DR   EnsemblFungi; EDP54927; EDP54927; AFUB_029870.
DR   VEuPathDB; FungiDB:AFUB_029870; -.
DR   HOGENOM; CLU_045277_6_2_1; -.
DR   Proteomes; UP000001699; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   Gene3D; 1.10.20.10; -; 1.
DR   InterPro; IPR027116; Bur6/Dpb3.
DR   InterPro; IPR003958; CBFA_NFYB_domain.
DR   InterPro; IPR009072; Histone-fold.
DR   PANTHER; PTHR10252:SF5; PTHR10252:SF5; 1.
DR   Pfam; PF00808; CBFD_NFYB_HMF; 1.
DR   SUPFAM; SSF47113; SSF47113; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Nucleus; Transcription; Transcription regulation.
FT   CHAIN           1..247
FT                   /note="NCT transcriptional regulatory complex subunit A"
FT                   /id="PRO_0000449613"
FT   REGION          1..31
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          48..82
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          212..247
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..26
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        52..76
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        216..247
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   247 AA;  27155 MW;  0B46EBF5840767C6 CRC64;
     MTDQDSTYRP RSPDLSTFQS SIPPAVNSPI YPLASPLAHR ASYDASPFFA PQYQQPPAPL
     GRVPQQYIPP FVDPNSPLSP DMARRSSRLA RATEVAPMPE VSYAQPVLSE EPQQLPPPPP
     QPNPVASIEV KTKFPVARIK RIMQADEDVG KVAQVTPIAV SKALELFMIS LVTKAAKEAR
     DRNSKRVTAT HLKQAVVKDE VLDFLADIIA KVPDQPAGGR KHDDDGSDQN EQPKRKRGGR
     RPKDDSD