NCTA_ASPFU
ID NCTA_ASPFU Reviewed; 247 AA.
AC Q4X095;
DT 22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=NCT transcriptional regulatory complex subunit A {ECO:0000303|PubMed:31969561};
DE AltName: Full=Negative cofactor 2 AB {ECO:0000303|PubMed:31969561};
GN Name=nctA {ECO:0000303|PubMed:31969561}; ORFNames=AFUA_2G14250;
OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS A1100) (Aspergillus fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=330879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=16372009; DOI=10.1038/nature04332;
RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA Barrell B.G., Denning D.W.;
RT "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT Aspergillus fumigatus.";
RL Nature 438:1151-1156(2005).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH NCTB AND MOT1, SUBCELLULAR
RP LOCATION, AND DNA-BINDING.
RX PubMed=31969561; DOI=10.1038/s41467-019-14191-1;
RA Furukawa T., van Rhijn N., Fraczek M., Gsaller F., Davies E., Carr P.,
RA Gago S., Fortune-Grant R., Rahman S., Gilsenan J.M., Houlder E.,
RA Kowalski C.H., Raj S., Paul S., Cook P., Parker J.E., Kelly S.,
RA Cramer R.A., Latge J.P., Moye-Rowley S., Bignell E., Bowyer P.,
RA Bromley M.J.;
RT "The negative cofactor 2 complex is a key regulator of drug resistance in
RT Aspergillus fumigatus.";
RL Nat. Commun. 11:427-427(2020).
CC -!- FUNCTION: Part of the NCT transcriptional regulatory complex that acts
CC as a key regulator of ergosterol biosynthesis and the azole exporter
CC cdr1B (PubMed:31969561). The NCT complex binds the promoters of genes
CC linked to azole susceptibility, and especially represses the expression
CC of cdr1B transporter (PubMed:31969561). {ECO:0000269|PubMed:31969561}.
CC -!- SUBUNIT: Forms the NCT transcriptional regulatory complex with nctB and
CC mot1. {ECO:0000269|PubMed:31969561}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:31969561}.
CC -!- DISRUPTION PHENOTYPE: Results in a reduction in growth rate
CC (PubMed:31969561). Leads to a multidrug-resistance phenotype, including
CC the azoles (itraconazole, voriconazole and posaconazole), as well as
CC the salvage therapeutic amphotericin B and terbinafine, an agent used
CC in the treatment of chronic and allergic disease (PubMed:31969561).
CC Leads also to transcriptional derepression of cdr1B and its over-
CC production (PubMed:31969561). Results also in a notable increase in the
CC immunogenic properties of Aspergillus fumigatus, but does not result in
CC loss of virulence (PubMed:31969561). {ECO:0000269|PubMed:31969561}.
CC -!- SIMILARITY: Belongs to the NC2 alpha/DRAP1 family. {ECO:0000305}.
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DR EMBL; AAHF01000001; EAL93720.1; -; Genomic_DNA.
DR RefSeq; XP_755758.1; XM_750665.1.
DR AlphaFoldDB; Q4X095; -.
DR SMR; Q4X095; -.
DR STRING; 746128.CADAFUBP00002926; -.
DR EnsemblFungi; EAL93720; EAL93720; AFUA_2G14250.
DR GeneID; 3512903; -.
DR KEGG; afm:AFUA_2G14250; -.
DR VEuPathDB; FungiDB:Afu2g14250; -.
DR eggNOG; KOG1659; Eukaryota.
DR HOGENOM; CLU_045277_6_2_1; -.
DR InParanoid; Q4X095; -.
DR OMA; MTDQDNS; -.
DR OrthoDB; 1504101at2759; -.
DR Proteomes; UP000002530; Chromosome 2.
DR GO; GO:0017054; C:negative cofactor 2 complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001046; F:core promoter sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IBA:GO_Central.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0003714; F:transcription corepressor activity; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR027116; Bur6/Dpb3.
DR InterPro; IPR003958; CBFA_NFYB_domain.
DR InterPro; IPR009072; Histone-fold.
DR PANTHER; PTHR10252:SF5; PTHR10252:SF5; 1.
DR Pfam; PF00808; CBFD_NFYB_HMF; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..247
FT /note="NCT transcriptional regulatory complex subunit A"
FT /id="PRO_0000449612"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 48..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 212..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..26
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..76
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 216..247
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 247 AA; 27155 MW; 0B46EBF5840767C6 CRC64;
MTDQDSTYRP RSPDLSTFQS SIPPAVNSPI YPLASPLAHR ASYDASPFFA PQYQQPPAPL
GRVPQQYIPP FVDPNSPLSP DMARRSSRLA RATEVAPMPE VSYAQPVLSE EPQQLPPPPP
QPNPVASIEV KTKFPVARIK RIMQADEDVG KVAQVTPIAV SKALELFMIS LVTKAAKEAR
DRNSKRVTAT HLKQAVVKDE VLDFLADIIA KVPDQPAGGR KHDDDGSDQN EQPKRKRGGR
RPKDDSD
